scholarly journals Detection of Three Avian Respiratory Viruses by Single-Tube Multiplex Reverse Transcription–Polymerase Chain Reaction Assay

2004 ◽  
Vol 16 (3) ◽  
pp. 244-248 ◽  
Author(s):  
Yashpal S. Malik ◽  
Devi P. Patnayak ◽  
Sagar M. Goyal
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Polymerase Chain Reaction Assay ◽  
Respiratory Viruses ◽  
Chain Reaction ◽  
Single Tube ◽  
Polymerase Chain

Multiplex Nested Reverse Transcription-Polymerase Chain Reaction for Respiratory Viruses in Acute Otitis Media

2003 ◽  
Vol 112 (3) ◽  
pp. 252-257 ◽  
Author(s):  
Toshio Ishibashi ◽  
Hiroko Monobe ◽  
Masanobu Shinogami ◽  
Yuka Nomura ◽  
Jun Yano
Keyword(s):  
Polymerase Chain Reaction ◽  
Otitis Media ◽  
Reverse Transcription ◽  
Acute Otitis Media ◽  
Respiratory Viruses ◽  
Molecular Technique ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Polymerase Chain

Because respiratory viruses play an important role in the causation and pathogenesis of acute otitis media (AOM), determining which virus has infected a child is important with respect to vaccines and antiviral drugs. In some instances, this information might be used to prevent the occurrence of AOM. We used a rapid, economical, and sensitive diagnostic system involving a multiplex nested reverse transcription–polymerase chain reaction (RT-PCR) assay to detect various respiratory viruses in clinical specimens of middle ear fluid (MEF) from children with AOM in our hospital. Multiplex RT-PCR was completed on 40 MEF samples from 28 infants and children less than 6 years old with AOM. Viral RNA was detected in 17 MEF samples (43%). Respiratory syncytial virus type A was present in 12 samples, adenovirus in 3, rhinovirus in 2, and influenza A (H3N2) in 1. The multiplex RT-PCR assay is recommended to clinical laboratories that are considering adoption of a molecular technique for viral diagnosis.

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