Development of One-Step Real-Time Reverse Transcription Polymerase Chain Reaction Assays for Rapid Detection of Porcine Group C Rotaviruses

2010 ◽  
Vol 22 (1) ◽  
pp. 74-77 ◽  
Author(s):  
Young-Hyun Chun ◽  
Young-Ju Jeong ◽  
Sang-Ik Park ◽  
Myra Hosmillo ◽  
Dong-Jun Shin ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Reverse Transcription ◽  
Rapid Detection ◽  
Chain Reaction ◽  
One Step ◽  
Polymerase Chain

Analysis of the persistence time of the SARS-CoV-2 virus in the cadaver and the risk of passing infection to autopsy staff

2021 ◽  
pp. 002581722098060
Author(s):  
Isabella Aquila ◽  
Pietrantonio Ricci ◽  
Carlo Filippo Bonetta ◽  
Matteo Antonio Sacco ◽  
Federico Longhini ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Reverse Transcription ◽  
Post Mortem ◽  
Forensic Pathologist ◽  
Chain Reaction ◽  
Sample Group ◽  
Persistence Time ◽  
One Step ◽  
Polymerase Chain

The activity of the SARS-CoV-2 virus has not yet been studied in a post-mortem setting. The absence of these data has led to the prohibition of exposure of infected corpses during burial procedures. Our aim was to assess the virus’s persistence and the possibility of transmission in the post-mortem phase including autopsy staff. The sample group included 29 patients who were admitted to our Covid-19 Centre who died during hospitalisation and the autopsy staff. All the swabs were subjected to a one-step real-time reverse transcription polymerase chain reaction with cycle threshold (Ct) values. Swab collection was performed at 2 h, 4 h, 6 h, 12 h, over 24 since death. The following were the analysis of patients’ swabs: 10 cases were positive 2 h after death; 10 cases positive 4 h after death; 9 cases were found positive 6 h after death; 7 cases positive 12 h after death; 9 cases remained positive 24 h after death. The swabs performed on all the forensic pathologist staff on duty who performed the autopsies were negative. The choice to avoid rituals and the display of corpses before and at the burial procedures given appears cautiously valid due to the persistence of the SARS-CoV-2 virus in the post-mortem period. Although the caution in choosing whether or not to perform an autopsy on infected corpses is acceptable, not to perform autopsies is not biologically supported.

scholarly journals Evaluation of immunochromatographic tests for the rapid detection of the emerging GII.17 norovirus in stool samples, January 2016

2016 ◽  
Vol 21 (4) ◽  
Author(s):  
Lucie Théry ◽  
Maxime Bidalot ◽  
Pierre Pothier ◽  
Katia Ambert-Balay
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Reverse Transcription ◽  
Rapid Detection ◽  
Virus Titre ◽  
Chain Reaction ◽  
Diagnostic Assays ◽  
Stool Samples ◽  
Polymerase Chain

A novel GII.17 norovirus emerged in Asia in the winter of 2014/15. A worldwide spread is conceivable and norovirus diagnostic assays need to be evaluated to investigate if they adequately detect this emerging genotype. Seven immunochromatographic kits commercially available in Europe were evaluated on ten stool samples where GII.17 virus had been quantified by real-time reverse transcription-polymerase chain reaction. All the kits detected GII.17 with various sensitivities, partly depending on the virus titre.

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