Miniaturization of Whole Live Cell-Based GPCR Assays Using Microdispensing and Detection Systems
2004 ◽
Vol 9
(3)
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pp. 186-195
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Keyword(s):
Cell-based β-lactamase reporter gene assays designed to measure the functional responses of G-protein-coupled receptors (GPCRs) were miniaturized to less than 2 μL total assay volume in a 3456-well microplate. Studies were done to evaluate both receptor agonists and antagonists. The pharmacology of agonists and antagonists for target GPCRs originally developed in a 96-well format was recapitulated in a 3456-well microplate format without compromising data quality or EC50/IC50 precision. These assays were employed in high-throughput screening campaigns, allowing the testing of more than 150,000 compounds in 8 h. The instrumentation used and practical aspects of the assay development are discussed.
2005 ◽
Vol 1
(1)
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pp. 97-111
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Keyword(s):
2005 ◽
pp. 159-189
2003 ◽
Vol 6
(4)
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pp. 389-397
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Keyword(s):
2008 ◽
Vol 13
(8)
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pp. 737-747
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2010 ◽
Vol 31
(12)
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pp. 1618-1624
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Keyword(s):
2012 ◽
pp. 97-111
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Keyword(s):
2000 ◽
Vol 5
(5)
◽
pp. 329-334
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2009 ◽
Vol 24
(7)
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pp. 2298-2304
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