Misdiagnoses of Tuberculosis Resulting from Laboratory Cross-Contamination of Mycobacterium Tuberculosis Cultures—New Jersey, 1998

2000 ◽  
Vol 5 (1) ◽  
pp. 30-32
Keyword(s):  
Mycobacterium Tuberculosis ◽  
New Jersey ◽  
Cross Contamination

Use of Spoligotype Analysis to Detect Laboratory Cross-Contamination

2000 ◽  
Vol 21 (8) ◽  
pp. 525-527 ◽  
Author(s):  
Beth Nivin ◽  
Jeffrey Driscoll ◽  
Tom Glaser ◽  
Pablo Bifani ◽  
Sonal Munsiff
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Dna Fingerprinting ◽  
False Positive ◽  
Cross Contamination

Spoligotype analysis identified false-positive isolates ofMycobacterium tuberculosiscaused by laboratory cross-contamination. Spoligotyping is faster, is less expensive than DNA fingerprinting, and can be used with a variety of media. Patients were reevaluated and had medications discontinued as a result of this investigation. Months of unnecessary patient follow-up and treatment were avoided.

scholarly journals Clonal Complexity in Mycobacterium tuberculosis Can Hamper Diagnostic Procedures

2017 ◽  
Vol 55 (5) ◽  
pp. 1388-1395 ◽  
Author(s):  
Laura Pérez-Lago ◽  
Marta Herranz ◽  
Yurena Navarro ◽  
María Jesús Ruiz Serrano ◽  
Pilar Miralles ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
High Risk ◽  
Mixed Infection ◽  
Diagnostic Procedures ◽  
Hiv Positive ◽  
Mixed Infections ◽  
Cross Contamination ◽  
Content Type ◽  
High Burden ◽  
Susceptibility Patterns

ABSTRACT Clonal complexity is increasingly accepted in Mycobacterium tuberculosis infection, including mixed infections by ≥2 strains, which usually occur in settings with a high burden of tuberculosis and/or a high risk of overexposure to infected patients. Mixed infections can hamper diagnostic procedures; obtaining an accurate antibiogram is difficult when the susceptibility patterns of the strains differ. Here, we show how mixed infections can also prove challenging for other diagnostic procedures, even outside settings where mixed infections are traditionally expected. We show how an unnoticed mixed infection in an HIV-positive patient diagnosed in Madrid, Spain, with differences in the representativeness of the coinfecting strains in different sputum samples, markedly complicated the resolution of a laboratory cross-contamination false positivity alert.

Cross-Contamination with Mycobacterium tuberculosis: An Epidemiological and Laboratory Investigation

1998 ◽  
Vol 19 (7) ◽  
pp. 500-503 ◽  
Author(s):  
Beth Nivin ◽  
Paula I. Fujiwara ◽  
John Hannifin ◽  
Barry N. Kreiswirth
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Laboratory Investigation ◽  
Cross Contamination

scholarly journals Laboratory Cross-Contamination of Mycobacterium tuberculosis: A Systematic Review and Meta-analysis

Lung ◽  
2019 ◽  
Vol 197 (5) ◽  
pp. 651-661 ◽  
Author(s):  
Aleksandra Barac ◽  
Hannah Karimzadeh-Esfahani ◽  
Mahya Pourostadi ◽  
Mohammad Taghi Rahimi ◽  
Ehsan Ahmadpour ◽  
...  
Keyword(s):  
Systematic Review ◽  
Mycobacterium Tuberculosis ◽  
Meta Analysis ◽  
Cross Contamination

The Effect of Changes in Laboratory Practices on the Rate of False-Positive Cultures for Mycobacterium tuberculosis

2001 ◽  
Vol 125 (9) ◽  
pp. 1213-1216
Author(s):  
Peter E. Breese ◽  
William J. Burman ◽  
Mary Hildred ◽  
Barbara Stone ◽  
Michael L. Wilson ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
False Positive ◽  
Positive Culture ◽  
Control Program ◽  
Dna Fingerprint ◽  
Cross Contamination ◽  
Convenience Sample ◽  
Hospital System ◽  
Policies And Procedures ◽  
Public Hospital System

Abstract Context.—False-positive cultures for Mycobacterium tuberculosis have been found in nearly all DNA fingerprinting studies, but the effectiveness of interventions to reduce cross-contamination has not been evaluated. Objective.—To evaluate whether changes in laboratory policies and procedures reduced the rate of false-positive cultures. Design.—Retrospective study of isolates with matching DNA fingerprints. Setting.—A mycobacteriology laboratory serving an urban tuberculosis control program and public hospital system. Patients.—All M tuberculosis isolates processed from July 1994 to December 1999. Methods.—Isolates were fingerprinted using IS6110; pTBN12 was used to fingerprint isolates having fewer than 6 copies of IS6110. We further evaluated all patients having only one positive culture whose DNA fingerprint matched that of another isolate processed in the laboratory within 42 days. Interventions.—We changed laboratory policy to reduce the number of smear-positive specimens processed and changed laboratory procedures to minimize the risk of cross-contamination during batch processing. Main Outcome Measure.—The rate of false-positive cultures. Results.—Of 13 940 specimens processed during the study period, 630 (4.5%) from 184 patients and 48 laboratory proficiency specimens grew M tuberculosis. There were no cases (0/184) of probable or definite cross-contamination, compared with the 4% rate (8/199) identified in our previous study (P = .008). We also fingerprinted a convenience sample of isolates from other laboratories in Denver; 13.6% (3/22) of these were false-positive, a rate similar to the 11.9% rate (5/42) identified for other laboratories in our previous study (P = .84). Conclusions.—Laboratory cross-contamination decreased significantly after relatively simple, inexpensive changes in laboratory policies and practices. Cross-contamination continued to occur in other laboratories in Denver.

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