In-vitro Analysis for Microbial Barrier Properties of 2-Octyl Cyanoacrylate-Derived Wound Treatment Films

2003 ◽  
Vol 7 (1) ◽  
pp. 13-19 ◽  
Author(s):  
Upvan Narang ◽  
Lawrence Mainwaring ◽  
Gina Spath ◽  
Joe Barefoot
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Drug Administration ◽  
Wound Care ◽  
Barrier Properties ◽  
Wound Treatment ◽  
The Media ◽  
Vitro Analysis ◽  
In Vitro Analysis

Background: In recent years, 2-octyl cyanoacrylate monomer has been formulated for various wound care products that have been approved by the Food and Drug Administration (FDA). Purpose: To evaluate the in vitro effectiveness of 2-octyl cyanoacrylate formulation-based films as barriers to various pathogens, including bacteria, fungi, and yeast. Methods: The barrier properties of the cyanoacrylate films prepared by the following two methods were tested: (1) prepolymerized film and (2) in situ polymerized film. The upper surface of films was inoculated such that the microorganisms would have to penetrate the film to colonize the media beneath. Nine different organisms were used. Plates were observed for growth at two, four, and seven days after inoculation. Results: No growth was observed in any test, with the exception of prepolymerized film challenged with Pseudomonas aeruginosa. The pattern of growth observed suggests that the bacteria colonized the medium by traveling around and not through the film. Conclusions: 2-Octyl cyanoacrylate-based films are excellent microbial barriers.

In vitro analysis of green fabricated silver nanoparticles (AgNPs) against Pseudomonas aeruginosa PA14 biofilm formation, their application on urinary catheter

2021 ◽  
Vol 151 ◽  
pp. 106058
Author(s):  
Felix LewisOscar ◽  
Chari Nithya ◽  
Sasikumar Vismaya ◽  
Manivel Arunkumar ◽  
Arivalagan Pugazhendhi ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Biofilm Formation ◽  
Urinary Catheter ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals In Vitro Analysis of an Optimal Procedure of Anterograde In Situ Laser Fenestration of Aortic Endografts

2019 ◽  
Vol 58 (6) ◽  
pp. e148
Author(s):  
Dorian Verscheure ◽  
Carlos Garcia Alonso ◽  
Philippe Brenot ◽  
Claude Angel ◽  
Stephan Haulon ◽  
...  
Keyword(s):  
Optimal Procedure ◽  
Vitro Analysis ◽  
In Vitro Analysis

The Nutritional Relationship of Anemonia Sulcata (Pennant) and its Dinoflagellate Symbiont

1969 ◽  
Vol 4 (3) ◽  
pp. 751-762
Author(s):  
D. L. TAYLOR
Keyword(s):  
Algal Symbiont ◽  
Carbon Translocation ◽  
Anemonia Sulcata ◽  
High Resolution Autoradiography ◽  
Electron Microscopes ◽  
Vitro Analysis ◽  
Relationship Of ◽  
In Vitro Analysis

High resolution autoradiography in the optical and electron microscopes has been used to define the nature of the nutritional relationship between Anemonia sulcata and its algal symbiont. Grain counts from these autoradiographs have been examined quantitatively, and the results compared with those obtained from an in vitro analysis of the excreted products of the symbionts. These investigations clearly indicate that system of carbon translocation between the alga and the host does exist in situ, and that over 60% of the carbon fixed in photosynthesis in transferred to the host under the conditions of these experiments.

In situ formation, structural, mechanical and in vitro analysis of ZrO2/ZnFe2O4 composite with assorted composition ratios

2020 ◽  
Vol 108 ◽  
pp. 110504 ◽  
Author(s):  
Subina Raveendran ◽  
M. Mushtaq Alam ◽  
Mohd. Imran K. Khan ◽  
Arunkumar Dhayalan ◽  
S. Kannan
Keyword(s):  
In Situ Formation ◽  
Composition Ratios ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals Folate Cross-Feeding Supports Symbiotic Homoacetogenic Spirochetes

2005 ◽  
Vol 71 (4) ◽  
pp. 1883-1889 ◽  
Author(s):  
Joseph R. Graber ◽  
John A. Breznak
Keyword(s):  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Community Members ◽  
Serratia Grimesii ◽  
Zootermopsis Angusticollis ◽  
Vitro Analysis ◽  
In Vitro Analysis ◽  
Exogenous Source

ABSTRACT Treponema primitia, an H2-consuming CO2-reducing homoacetogenic spirochete in termite hindguts, requires an exogenous source of folate for growth. Tetrahydrofolate (THF) acts as a C1 carrier in CO2-reductive acetogenesis, a microbially mediated process important to the carbon and energy requirements of termites. To examine the hypothesis that other termite gut microbes probably supply some form of folate to T. primitia in situ, we used a bioassay to screen for and isolate folate-secreting bacteria from hindguts of Zootermopsis angusticollis, which is the host of T. primitia. Based on morphology, physiology, and 16S rRNA gene sequences, the major folate secretors were identified as strains of Lactococcus lactis and Serratia grimesii. During growth, these isolates secreted 5-formyl-THF at levels up to 146 ng/ml, and their cell-free culture fluids satisfied the folate requirement of T. primitia strains in vitro. Analysis of Z. angusticollis hindgut fluid revealed that 5-formyl-THF was the only detectable folate compound and occurred at an in situ concentration (1.3 μg/ml) which was more than sufficient to support the growth of T. primitia. These results imply that cross-feeding of 5-formyl-THF by other community members is important for growth of symbiotic hindgut spirochetes and thus termite nutrition and survival.

Osteoclastogenesis in fibrous dysplasia of bone: in situ and in vitro analysis of IL-6 expression

Bone ◽  
2003 ◽  
Vol 33 (3) ◽  
pp. 434-442 ◽  
Author(s):  
M Riminucci ◽  
S.A Kuznetsov ◽  
N Cherman ◽  
A Corsi ◽  
P Bianco ◽  
...  
Keyword(s):  
Fibrous Dysplasia ◽  
Fibrous Dysplasia Of Bone ◽  
Vitro Analysis ◽  
In Vitro Analysis
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