scholarly journals Determination of Antiplasmodial Activity and Binding Affinity of Selected Natural Products towards PfTrxR and PfGR

2013 ◽  
Vol 8 (8) ◽  
pp. 1934578X1300800
Author(s):  
Ranjith Munigunti ◽  
Katja Becker ◽  
Reto Brun ◽  
Angela I. Calderón
Keyword(s):  
Mass Spectrometry ◽  
Natural Products ◽  
Ligand Binding ◽  
Binding Assay ◽  
Antimalarial Activity ◽  
Antiplasmodial Activity ◽  
Binding Affinities ◽  
Ligand Binding Assay

In our study, the binding affinities of selected natural products towards PfTrxR, PfGR, human TrxR and human GR were determined using a mass spectrometry based ligand binding assay. The in vitro antimalarial activity and cytotoxicity of these ligands were also determined. Catharanthine, 11-(OH)-coronaridine, hernagine, vobasine and hispolone displayed antiplasmodial activity against PfK1 (IC50 = 0.996–3.63 μg/mL).

Development of a nano-SiO 2 based enzyme-linked ligand binding assay for the determination of ibuprofen in human urine

Talanta ◽  
2017 ◽  
Vol 167 ◽  
pp. 617-622 ◽  
Author(s):  
Qian-Long Wang ◽  
Jing Xie ◽  
Xing-De Li ◽  
Li-Sheng Ding ◽  
Jian Liang ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Human Urine ◽  
Binding Assay ◽  
Ligand Binding Assay

scholarly journals Aktivitas Antimalaria Ekstrak Kasar Etanol dan Fraksi n-Heksana Rumput Bambu (Lophatherum gracile B.) secara in Vitro

ALCHEMY ◽  
2018 ◽  
Vol 6 (1) ◽  
pp. 18
Author(s):  
Ella Wulandari ◽  
Dewi Yuliani ◽  
Elok Kamilah Hayati ◽  
Roihatul Muti'ah
Keyword(s):  
Mass Spectrometry ◽  
Plasmodium Falciparum ◽  
Liquid Chromatography ◽  
Antimalarial Activity ◽  
Ethanol Extract ◽  
Hexane Fraction ◽  
Liquid Chromatography Mass Spectrometry ◽  
Mosquito Bite

<p>Malaria is a disease caused by infectious parasite <em>Plasmodium falciparum</em> and can be transmitted through mosquito bite. The aim of this research was to study antimalarial activity <em>in vitro</em> on crude ethanol extract and n-hexane fraction of bamboo grass (<em>Lophatherum gracile</em> B.). Extraction was carried out by ethanol 80% solvent and fractionation was conducted by n-hexane. Determination of antimalarial activity was subjected to <em>P. falciparum</em> strain 3D7. According to phytochemical test, crude ethanol extract contained tannin and terpenoid, whilst n-hexane fraction contained tannin and steroid. The capability of crude ethanol extract and n-hexane fraction to inhibit <em>P. falciparum</em> was represented by IC<sub>50</sub> value. The value of both samples respectively was 12.49 and 61.49 µg/mL. Identification based on LC-MS (liquid chromatography-mass spectrometry), n-hexane fraction shown the presence of tannin and steroid compounds.</p><p>  </p><p>Malaria merupakan penyakit yang disebabkan oleh infeksi parasit <em>Plasmodium falciparum</em> yang dapat ditularkan melalui gigitan nyamuk. Penelitian ini bertujuan untuk mengetahui aktivitas antimalaria secara <em>in vitro</em> pada ekstrak kasar etanol dan fraksi n-heksana rumput bambu (<em>L</em><em>ophat</em><em>h</em><em>erum gracile </em>B.). Proses ekstraksi dilakukan dengan pelarut etanol 80% dan fraksinasi dengan n-heksana. Uji aktivitas antimalaria dilakukan pada parasit <em>P. falciparum </em><em>strain</em> 3D7<em>.</em> Hasil uji fitokimia menunjukkan ekstrak etanol mengandung tanin dan terpenoid, sedangkan fraksi n-heksana mengandung tanin dan steroid. Kemampuan ekstrak etanol dan fraksi n-heksana dalam menghambat parasit <em>P. falciparum</em> menghasilkan nilai IC<sub>50</sub> masing-masing sebesar 12,49 dan 61,49 µg/mL. Identifikasi senyawa dengan KC-SM (kromatografi cair-spektrometri massa) pada fraksi n-heksana menunjukkan adanya senyawa tanin dan steroid.</p>

scholarly journals Single-point versus five-point biochemical method for determination of estrogen and progesterone receptors

2002 ◽  
Vol 21 (1) ◽  
pp. 15-20
Author(s):  
Dragica Nikolic-Vukosavljevic ◽  
Milan Markicevic ◽  
Sladjana Todorovic
Keyword(s):  
Quality Control ◽  
Breast Carcinoma ◽  
Ligand Binding ◽  
Binding Assay ◽  
Single Point ◽  
Progesterone Receptors ◽  
Medical Decision ◽  
Ligand Binding Assay ◽  
Estrogen And Progesterone Receptors

Receptors for estrogen and progesterone are accepted by international consensus as biomarkers of breast carcinoma responsiveness to endocrine therapy. Numerous current studies are aimed at consideration of importance of "the new generation" of estrogen-regulated biomarkers in treatment of breast cancer patients. Simultaneous knowledge of all these biomarkers may help in medical decision making. However, the amount of tumor material available from breast carcinoma can make impossible determination of estrogenregulated biomarkes together with estrogen and progesterone receptors. To assess whether we could replace our current five-point ligand binding assay for measurement of estrogen and progesterone receptors with a single- point ligand binding assay, we compared simultaneous measurements in same samples of breast carcinomas by both methods. A linear regression analysis shows that single-point assay can be confidently used instead of five-point assay. In addition, there were no variations over time in estrogen and progesterone receptors phenotypes, as well as in estrogen and progesterone receptors contents determined by single-point assay. Accordingly, the results clearly demonstrate the validity of intralaboratory quality control and give a possibility for the establishment of interlaboratory quality control of single-point ligand binding assay.

TBG MEASUREMENTS BY COMPETITIVE LIGAND BINDING ASSAY (CLBA)

1975 ◽  
Vol 80 (1_Suppla) ◽  
pp. S15
Author(s):  
K. H. Rudorff ◽  
H. J. Kröll ◽  
J. Herrmann
Keyword(s):  
Ligand Binding ◽  
Binding Assay ◽  
Ligand Binding Assay ◽  
Competitive Ligand
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