scholarly journals Improved visualization of luteinizing hormone releasing hormone pathways by immunocytochemical staining of thick vibratome sections.

1980 ◽  
Vol 28 (4) ◽  
pp. 361-363 ◽  
Author(s):  
B J Burchanowski ◽  
L A Sternberger
Keyword(s):  
Luteinizing Hormone ◽  
Median Eminence ◽  
Anterior Commissure ◽  
Third Ventricle ◽  
Immunocytochemical Staining ◽  
Luteinizing Hormone Releasing Hormone ◽  
Bed Nucleus ◽  
Releasing Hormone ◽  
The Third ◽  
Diagonal Band

Using 100-micron thick Vibratome sections and a modification of the peroxidase--antiperoxidase method of immunocytochemical staining we achieve a Golgi-like image of luteinizing hormone releasing hormone (LHRH) cells and fibers in mouse brain. Five LHRH pathways are described: 1) A dense projection of fibers from LHRH cells in the medial preoptic and septal areas to the wall of the third ventricle; 2) a projection of fibers from neurons in the bed nucleus of the stria terminalis and the nucleus of the anterior commissure to the subfornical organ; 3) projections of fibers from neurons in the medial septal nucleus and the diagonal band of Broca to the olfactory bulb; 4) fibers which travel within or just lateral to the wall of the third ventricle from the organum vasculosum laminae terminalis to the median eminence; 5) cells and fibers located just dorsal to the optic tracts which project rostrally to the preoptic area and caudally to the level of the median eminence where they course medially to converge and enter the median eminence.

scholarly journals Luteinizing Hormone-Releasing Hormone Distribution in the Anterior Hypothalamus of the Female Rats

ISRN Anatomy ◽  
2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Leandro Castañeyra-Ruiz ◽  
Ibrahim González-Marrero ◽  
Agustín Castañeyra-Ruiz ◽  
Juan M. González-Toledo ◽  
María Castañeyra-Ruiz ◽  
...  
Keyword(s):  
Luteinizing Hormone ◽  
Preoptic Area ◽  
Third Ventricle ◽  
Anterior Hypothalamus ◽  
Lamina Terminalis ◽  
Luteinizing Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
Immunoreactive Material ◽  
The Third ◽  
Organum Vasculosum

Luteinizing hormone-releasing hormone (LHRH) neurons and fibers are located in the anteroventral hypothalamus, specifically in the preoptic medial area and the organum vasculosum of the lamina terminalis. Most luteinizing hormone-releasing hormone neurons project to the median eminence where they are secreted in the pituitary portal system in order to control the release of gonadotropin. The aim of this study is to provide, using immunohistochemistry and female brain rats, a new description of the luteinizing hormone-releasing hormone fibers and neuron localization in the anterior hypothalamus. The greatest amount of the LHRH immunoreactive material was found in the organum vasculosum of the lamina terminalis that is located around the anterior region of the third ventricle. The intensity of the reaction of LHRH immunoreactive material decreases from cephalic to caudal localization; therefore, the greatest immunoreaction is in the organum vasculosum of the lamina terminalis, followed by the dorsomedial preoptic area, the ventromedial preoptic area, and finally the ventrolateral medial preoptic area, and in fibers surrounding the suprachiasmatic nucleus and subependymal layer on the floor of the third ventricle where the least amount immunoreactive material is found.

scholarly journals Receptors for luteinizing hormone-releasing hormone.

1978 ◽  
Vol 26 (7) ◽  
pp. 542-544 ◽  
Author(s):  
L A Sternberger
Keyword(s):  
Luteinizing Hormone ◽  
Secretory Granules ◽  
Immunocytochemical Staining ◽  
Luteinizing Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
High Affinity ◽  
Strong Staining ◽  
Lh Rh

Antisera to luteinizing hormone-releasing hormone (LH-RH) confer on Araldite sections of occasional rat pituitaries moderate immunocytochemical staining to the large secretory granules of gonadotrophs. Treatment of the sections with LH-RH before anti-LH-RH yields strong staining in all animals, irrespective of presence or absence of staining without pretreatment. This enhancement of staining is specific for LH-RH and is a high affinity, saturable reaction. Staining with or without LH-RH pretreatment is absent when anti-LH-RH absorbed with insolubilized LH-RH is used. Staining is inhibited by carboxyterminally-deficient LH-RH, unaffected by aminoterminally deficient LH-RH.

scholarly journals Immunocytochemical analysis of the rat pineal gland using antisera generated against analogs of luteinizing hormone-releasing hormone (LHRH).

1982 ◽  
Vol 30 (2) ◽  
pp. 106-110 ◽  
Author(s):  
D T Piekut ◽  
K M Knigge
Keyword(s):  
Luteinizing Hormone ◽  
Pineal Gland ◽  
Median Eminence ◽  
Luteinizing Hormone Releasing Hormone ◽  
Immunocytochemical Analysis ◽  
Releasing Hormone ◽  
Immunoreactive Material ◽  
Lhrh Agonists ◽  
C Terminus ◽  
Rat Pineal Gland

Antisera generated against synthetic luteinizing hormone-releasing hormone (LHRH) or analogs of LHRH, agonists and antagonist, were used to stain the rat pineal gland and the median eminence of the hypothalamus. Of the LHRH analogs used, two revealed immunoreactive material in median eminence and not pineal, two stained pineal only, and three stained median eminence and pineal. Our observations suggest that the immunoreactive material in the pineal gland may be an LHRH-like substance whose affinity characteristics are different from that of hypothalamic LHRH. The affinity properties of hypothalamic LHRH for antibody appear to reside in the C-terminus of the molecule, whereas binding of the antigen in the pineal to antibody appear to be influenced by an N-terminal modification of the LHRH molecule.

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