scholarly journals Immunocytochemical localization of cytoskeletal proteins and histone 2B in isolated membrane-depleted nuclei, metaphase chromatin, and whole Chinese hamster ovary cells.

1983 ◽  
Vol 31 (12) ◽  
pp. 1385-1393 ◽  
Author(s):  
B L Armbruster ◽  
H Wunderli ◽  
B M Turner ◽  
I Raska ◽  
E Kellenberger
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Protein A ◽  
Chinese Hamster ◽  
Cytoskeletal Proteins ◽  
Metaphase Chromosomes ◽  
Thin Sections ◽  
Ovary Cells ◽  
Whole Cells ◽  
Immunocytochemical Techniques

Immunocytochemical techniques employing protein A-gold labeling were used to locate actin, tubulin, and histone 2B in thin sections of embedded, isolated membrane-depleted nuclei, metaphase chromosomes, and whole Chinese hamster ovary (CHO) cells. Actin and tubulin were detected in significant amounts in the cytoplasm and the nucleus of whole cells. The isolated membrane-depleted nuclei and metaphase chromosomes showed levels of actin and tubulin labeling either comparable to or sometimes lower than the levels of labeling in whole interphase cells. The results suggest that actin and tubulin are normal components of nuclei throughout the cell cycle. A mouse monoclonal antibody to histone 2B gave relatively weak labeling of nuclei and chromosomes in whole cells but intense labeling of isolated nucleoids and chromosomes. An increased concentration of antibody at the periphery of interphase nucleoids was noted.

scholarly journals Accessibility of metaphase chromosomes from chinese hamster ovary cells to DNase II

FEBS Letters ◽  
1981 ◽  
Vol 132 (2) ◽  
pp. 341-343 ◽  
Author(s):  
F. Fittler ◽  
K. Ibel ◽  
W. Hörz
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Metaphase Chromosomes ◽  
Dnase Ii ◽  
Ovary Cells

Regulation of Na+/Ca2+exchange activity by cytosolic Ca2+ in transfected Chinese hamster ovary cells

1998 ◽  
Vol 275 (1) ◽  
pp. C50-C55 ◽  
Author(s):  
Yu Fang ◽  
Madalina Condrescu ◽  
John P. Reeves
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Hill Coefficient ◽  
Acetoxymethyl Ester ◽  
Ovary Cells ◽  
Whole Cells ◽  
Order Of Magnitude ◽  
The Difference ◽  
Exchange Activity

Transfected Chinese hamster ovary cells stably expressing the bovine cardiac Na+/Ca2+exchanger (CK1.4 cells) were used to determine the range of cytosolic Ca2+ concentrations ([Ca2+]i) that activate Na+/Ca2+exchange activity. Ba2+ influx was measured in fura 2-loaded, ionomycin-treated cells under conditions in which the intracellular Na+concentration was clamped with gramicidin at ∼20 mM. [Ca2+]iwas varied by preincubating ionomycin-treated cells with either the acetoxymethyl ester of EGTA or medium containing 0–1 mM added CaCl2. The rate of Ba2+ influx increased in a saturable manner with [Ca2+]i, with the half-maximal activation value of 44 nM and a Hill coefficient of 1.6. When identical experiments were carried out with cells expressing a Ca2+-insensitive mutant of the exchanger, Ba2+influx did not vary with [Ca2+]i. The concentration for activation of exchange activity was similar to that reported for whole cardiac myocytes but approximately an order of magnitude lower than that reported for excised, giant patches. The reason for the difference in Ca2+regulation between whole cells and membrane patches is unknown.

scholarly journals Cell cycle-dependent phosphorylation and microtubule binding of tau protein stably transfected into Chinese hamster ovary cells.

1995 ◽  
Vol 6 (10) ◽  
pp. 1397-1410 ◽  
Author(s):  
U Preuss ◽  
F Döring ◽  
S Illenberger ◽  
E M Mandelkow
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Tau Protein ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Protein A ◽  
Chinese Hamster ◽  
Paired Helical Filaments ◽  
Ovary Cells

Tau protein, a neuronal microtubule-associated protein, is phosphorylated in situ and hyperphosphorylated when aggregated into the paired helical filaments of Alzheimer's disease. To study the phosphorylation of tau protein in vivo, we have stably transfected htau40, the largest human tau isoform, into Chinese hamster ovary cells. The distribution and phosphorylation of tau was monitored by gel shift, autoradiography, immunofluorescence, and immunoblotting, using the antibodies Tau-1, AT8, AT180, and PHF-1, which are sensitive to the phosphorylation of Ser202, Thr205, Thr231, Ser235, Ser396, and Ser404 and are used in the diagnosis of Alzheimer tau. In interphase cells, tau becomes phosphorylated to some extent, partly at these sites; most of the tau is associated with microtubules. In mitosis, the above Ser/Thr-Pro sites become almost completely phosphorylated, causing a pronounced shift in M(r) and an antibody reactivity similar to that of Alzheimer tau. Moreover, a substantial fraction of tau is found in the cytoplasm detached from microtubules. Autoradiographs of metabolically labeled Chinese hamster ovary cells in interphase and mitosis confirmed that tau protein is more highly phosphorylated during mitosis. The understanding of tau phosphorylation under physiological conditions might help elucidate possible mechanisms for the hyperphosphorylation in Alzheimer's disease.

Chronic Morphine Up-Regulates Gα12 and Cytoskeletal Proteins in Chinese Hamster Ovary Cells Expressing the Cloned μ Opioid Receptor

2005 ◽  
Vol 315 (1) ◽  
pp. 248-255 ◽  
Author(s):  
Heng Xu ◽  
Xiaoying Wang ◽  
Darin Zimmerman ◽  
Emily S. Boja ◽  
Jiabei Wang ◽  
...  
Keyword(s):  
Opioid Receptor ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Cytoskeletal Proteins ◽  
Chronic Morphine ◽  
Ovary Cells ◽  
Μ Opioid Receptor

Expression ofStreptococcus mutansWall-Associated Protein A Gene in Chinese Hamster Ovary Cells: Prospect for a Dental Caries DNA Vaccine

2001 ◽  
Vol 20 (9) ◽  
pp. 595-601 ◽  
Author(s):  
Thomas K. Han ◽  
Sean Yoder ◽  
Chuanhai Cao ◽  
Kenneth E. Ugen ◽  
My Lien Dao
Keyword(s):  
Dental Caries ◽  
Dna Vaccine ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Protein A ◽  
Chinese Hamster ◽  
Ovary Cells

Expression and characterization of rat surfactant protein A synthesized in Chinese hamster ovary cells

1990 ◽  
Vol 1087 (2) ◽  
pp. 190-198 ◽  
Author(s):  
Francis X. McCormack ◽  
James H. Fisher ◽  
Akira Suwabe ◽  
David L. Smith ◽  
John M. Shannon ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Protein A ◽  
Chinese Hamster ◽  
Surfactant Protein ◽  
Surfactant Protein A ◽  
Ovary Cells

scholarly journals Direct biochemical measurements of microtubule assembly and disassembly in Chinese hamster ovary cells. The effect of intercellular contact, cold, D2O, and N6,O2'-dibutyryl cyclic adenosine monophosphate.

1975 ◽  
Vol 64 (1) ◽  
pp. 42-53 ◽  
Author(s):  
R W Rubin ◽  
G D Weiss
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Binding Activity ◽  
Microtubule Assembly ◽  
Thin Sections ◽  
Ovary Cells ◽  
Significant Difference

A study was undertaken to develop a means of quantitating the amount of tubulin present as a soluble pool and as intact microtubules in cultured Chinese hamster ovary cells. A procedure was developed in which these cells grown on monolayer culture in Petri dishes were placed in a "microtubule stabilizing medium" (MTM) consisting of 50% glycerol, 10% dimethylsulfoxide and sodium phosphate magnesium buffer, as described previously by Filner and Behnke. These cells then were homogenized and the homogenate was spun in the ultracentrifuge. Colchicine binding activity was then determined in the supernates and the pellets. The values, when compared with total colchicine binding activity present in replicate homogenates, were used to determine the percentage of tubulin present as intact microtubules. A statistical analysis of thin sections of cells treated with MTM revealed no statistically significant difference between MTM-treated cells and untreated controls. It was further discovered that the relative amount of colchicine binding activity recovered in the high speed pellet varied dramatically, depending upon the cell number of the culture being studied. Preconfluent cultures showed very low colchicine binding activity averaging less than 5%, while confluent and postconfluent cultures often possessed as high as 25% of their total colchicine binding activity in pelletable material. Although cold and D2O treatment had little or no effect on these values, N6,O2'-dibutyryl cyclic adenosine monophosphate increased them. It is hoped that this study will serve as the basis for a reliable quantitative procedure for measuring microtubule polymerization and depolymerization in vivo.

Cytotoxicity of Vanadate on Chinese Hamster Ovary Cells

1987 ◽  
Vol 14 (3) ◽  
pp. 152-155
Author(s):  
J. Jenssen ◽  
T. Syversen
Keyword(s):  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Chinese Hamster Ovary Cells ◽  
Protein A ◽  
Chinese Hamster ◽  
Neutral Red ◽  
Cellular Protein ◽  
Similar Reduction ◽  
Ovary Cells

The cytotoxicity of vanadate was tested in CHO-cells using uptake of neutral red (NR) and the MIT test as indicators. Cells were exposed to 1–500μM monovanadate for 2–72 hours. Cells exposed to 100μM monovanadate caused a 50% reduction in MIT activity measured after 30 hours, while a similar reduction in NR uptake was observed after 16 hours exposure to the same vanadate concentration. Monovanadate also caused a reduction in amount of cellular protein; a 50% reduction was observed after 24 hours exposure to 100μM monovanadate.

Malignancy-related characteristics of wild type and drug-resistant chinese hamster ovary cells

Pathology ◽  
1993 ◽  
Vol 25 (3) ◽  
pp. 268-276 ◽  
Author(s):  
Wanda B. Mackinnon ◽  
Marlen Dyne ◽  
Rebecca Hancock ◽  
Carolyn E. Mountford ◽  
Adrienne J. Grant ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Drug Resistant ◽  
Wild Type ◽  
Ovary Cells
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