scholarly journals Immunolabeling of grapevine flavescence dorée MLO in salivary glands of Euscelidius variegatus: a light and electron microscopy study.

1990 ◽  
Vol 38 (1) ◽  
pp. 79-85 ◽  
Author(s):  
J Lherminier ◽  
G Prensier ◽  
E Boudon-Padieu ◽  
A Caudwell
Keyword(s):  
Light Microscopy ◽  
Salivary Glands ◽  
Light And Electron Microscopy ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Good Tool ◽  
Tissue Samples ◽  
Thin Sections ◽  
Flavescence Dorée ◽  
Euscelidius Variegatus

Flavescence dorée (FD), a grapevine yellows disease, is caused by a mycoplasma-like organism (MLO). A colloidal gold indirect immunolabeling technique identified MLO in salivary glands of a vector leafhopper, Euscelidius variegatus. After aldehyde fixation, tissue samples were prepared by cryoultramicrotomy or embedding in acrylic resins. Double fixation with aldehydes and osmium retroxide, followed by embedding in epon, was also performed. Thin or semi-thin serial sections were treated with polyclonal anti-FD-MLO rabbit antibodies, then with gold-conjugated anti-rabbit IgG. Labeling was revealed using the silver enhancement technique for light microscopy. MLO in frozen thin sections of glands were efficiently labeled. Optimal results were obtained with 4% paraformaldehyde-0.1% glutaraldehyde fixation and low-temperature embedding in LR White resin. Both scattered MLO and unusual dense forms of MLO were easily detected with the electron-dense gold probe. This method distinguished MLO from other membrane-limited bodies and provided a good tool for studying infection in large regions of FD-infected tissues by light microscopy.

Unusual Morphologic Features of a Primary Liver Neoplasm

1979 ◽  
Vol 65 (5) ◽  
pp. 649-655 ◽  
Author(s):  
Corrado Schmid ◽  
Luciano Lombardi ◽  
Ferdinando Giordano ◽  
Secondo Delgrossi
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Needle Biopsy ◽  
Light And Electron Microscopy ◽  
Liver Neoplasm ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Multinucleated Cells

Light and electron microscopy study of an unusual primary liver neoplasm observed in a 36-year-old woman. By light microscopy the tumor, initially detected by a needle biopsy and later surgically removed, was mainly composed of spindle-shaped and multinucleated cells which were similar to rhabdomyoblasts. These cells, however, showed ultrastructural features of hepatocytes.

Unusual Inclusions in Neutrophils of two Human Renal Allografts

1976 ◽  
Vol 34 ◽  
pp. 242-243
Author(s):  
R. Valenzuela ◽  
S.D. Deodhar ◽  
D.G. Osborne ◽  
W.E. Braun ◽  
L.H.W. Banowsky
Keyword(s):  
Electron Microscopy ◽  
Osmium Tetroxide ◽  
Renal Allograft ◽  
Light And Electron Microscopy ◽  
Microscopy Study ◽  
Uranyl Acetate ◽  
Electron Microscopy Study ◽  
Thin Sections ◽  
Blood Disorder ◽  
Cadaver Donor

During a retrospective light and electron microscopy study of 158 human renal allograft biopsies, we observed, in two cases, unusual inclusions in the neutrophils trapped in glomeruli and paratubular capillaries. For electron microscopy, the specimens were fixed in glutaraldehyde, post-fixed in osmium tetroxide and embedded in Epon. Thin sections were stained with uranyl acetate and lead citrate. Eighty-six of the electron microscopically examined biopsies did not contain any neutrophils for evaluation, and only 10 biopsies showed four or more neutrophils.The two patients, A age 35 and B age 42, had received a cadaver donor renal allograft because of chronic renal failure secondary to hereditary nephritis and nephrosclerosis respectively. Neither patient was affected by any known primary blood disorder.

scholarly journals Light and electron microscopy study of late embryonic development in the land snail Limicolaria flammea (Müller) (Pulmonata, Achatinidae)

2007 ◽  
Vol 24 (2) ◽  
pp. 436-441 ◽  
Author(s):  
Rosemary I. Egonmwan
Keyword(s):  
Electron Microscopy ◽  
Embryonic Development ◽  
Light And Electron Microscopy ◽  
Microscopy Study ◽  
Land Snail ◽  
Electron Microscopy Study ◽  
Advanced Embryo ◽  
Embryonic Shell ◽  
Advanced Stages ◽  
Late Stages

The late stages of embryogenesis in the achatinid land snail Limicolariaflammea (Müller, 1774) were described using light and electron microscopy. Embryos at various stages of development were present in the eggs during the first hour after they were laid, from 4-cell blastulae to morulae and fairly advanced stages. The advanced embryo which was fully developed on the second day bears a long cephalic sac, first to be developed, attached to the embryo and a podocyst which is attached to the foot of the embryo. Both of these structures are reduced in size as embryogenesis progresses until they finally disappear at about the 7th day after the egg was deposited. The embryonic shell was apparent on the second day and spiral coiling was apparent at about day 5. The spiral shell had one whorl when formed and more spirals were added so that at hatching the young snails had three whorls.

The Kupffer cell in experimental extrahepatic cholestasis in the rat—a light microscopy, immunohistochemical and electron microscopy study

1986 ◽  
Vol 150 (3) ◽  
pp. 187-194 ◽  
Author(s):  
D. St. John Collier ◽  
James A. Pain ◽  
Derek G. D. Wight ◽  
Penny Lovat ◽  
Michael E. Bailey
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Kupffer Cell ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Extrahepatic Cholestasis

Meningiomas. A Light and Electron Microscopy Study

1981 ◽  
pp. 122-125 ◽  
Author(s):  
Filomeno Tedeschi ◽  
R. Brizzi ◽  
A. Lechi ◽  
G. Trabattoni ◽  
C. Ferrari ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Microscopy Study ◽  
Electron Microscopy Study

Fine structure of Metchnikovella incurvata Caullery and Mesnil 1914 (microsporidia), a hyperparasite of gregarines Polyrhabdina sp. from the polychaete Pygospio elegans

Parasitology ◽  
2013 ◽  
Vol 140 (7) ◽  
pp. 855-867 ◽  
Author(s):  
Y. Y. SOKOLOVA ◽  
G. G. PASKEROVA ◽  
Y. M. ROTARI ◽  
E. S. NASSONOVA ◽  
A. V. SMIRNOV
Keyword(s):  
Electron Microscopy ◽  
Marine Invertebrates ◽  
Light And Electron Microscopy ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Life Cycles ◽  
Single Family ◽  
Host Cytoplasm ◽  
History Of ◽  
Multinuclear Cells

SUMMARYClass Rudimicrosporea Sprague 1977, with its single family Metchnikovellidae, comprises hyperparasites of gregarines from the guts of marine invertebrates. Metchnikovellids remain poorly studied in spite of their significance to the evolutionary history of microsporidia; their ultrastructure and life cycles require further investigation. Here we present results of the light- and electron-microscopy study of Metchnikovella incurvata Caulleri and Mesnil 1914, isolated from lecudinid gregarines, parasitizing polychaetes Pygospio elegans in the White Sea littoral zone, and yet described only on the light-microscopic level. The life cycle of this microsporidium includes 2 sporogonies: free (FS) and sac-bound (SBS). In FS, sporonts develop into multinuclear cells (sporogonial plasmodia), which generate sporoblasts and free spores residing in direct contact with the host cytoplasm. Electron microscopy revealed their metchnikovellidean structure: a horseshoe-shaped nucleus, short manubrium perpendicular to the long axis of the spore, and a polar cap in a separate membrane container. Merogony was not observed. The earliest stages of SBS were chains of binucleate cells. They underwent a series of nuclear and cell divisions, produced extracellular envelopes, and split into boomerang-shaped spore sacs, containing up to 16 spores each. Ultrastructure and sizes of sac-bounded spores were similar to those of free-living ones. An amended diagnosis of M. incurvata is provided.

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