scholarly journals Immunogold-silver staining method at the transmission electron microscopic level.

1991 ◽  
Vol 39 (3) ◽  
pp. 385-386 ◽  
Author(s):  
G C Manara
Keyword(s):  
Silver Staining ◽  
Staining Method ◽  
Microscopic Level ◽  
Electron Microscopic Level ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Transmission Electron Microscopic ◽  
Silver Staining Method

scholarly journals A New Method to Enhance Contrast of Ultrathin Cryosections for Immunoelectron Microscopy

2003 ◽  
Vol 51 (1) ◽  
pp. 31-39 ◽  
Author(s):  
Toshihiro Takizawa ◽  
Clark L. Anderson ◽  
John M. Robinson
Keyword(s):  
Immunoelectron Microscopy ◽  
Staining Method ◽  
Microscopic Level ◽  
New Method ◽  
Electron Microscopic Level ◽  
Positive Staining ◽  
Immunocytochemical Localization ◽  
Electron Microscopic ◽  
Morphological Detail ◽  
Ultrathin Cryosections

Adequate contrast of ultrathin cryosections is crucial for evaluating morphological detail to assess immunocytochemical localization at the electron microscopic level. We have developed a positive staining method for achieving contrast in ultrathin cryosections, from tissue fixed only in paraformaldehyde, that provides excellent contrast at the electron microscopic level.

scholarly journals Novel staining method for transmission electron microscopic investigations of semicrystalline polyesters

1993 ◽  
Vol 26 (4) ◽  
pp. 864-866 ◽  
Author(s):  
D. M. Huong ◽  
M. Drechsler ◽  
H. J. Cantow ◽  
M. Moeller
Keyword(s):  
Staining Method ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Transmission Electron Microscopic ◽  
Microscopic Investigations

Ultrastructural localization of nucleolar material by a simple silver staining technique devised for plant cells

1985 ◽  
Vol 79 (1) ◽  
pp. 259-269
Author(s):  
S. Sato
Keyword(s):  
Silver Staining ◽  
Secondary Constriction ◽  
Plant Cells ◽  
Staining Technique ◽  
Microscopic Level ◽  
Electron Microscopic Level ◽  
Electron Microscopic ◽  
Late Anaphase ◽  
Nucleolar Material ◽  
Silver Staining Technique

A simple silver staining technique for use at the electron microscopic level, consisting only of treatment with aqueous silver nitrate at high temperature for a prolonged time, was applied to thin sections of root tip meristems of Vicia faba. This technique contrasted the fibrillar component and the granular component in interphase nucleoli as a reflection of the degree of packing. In contrast, silver impregnation was scarcely discerned in chromosomes. A comparison of silver staining and conventional double staining showed that the fibrillar centres did not always respond positively to silver. During the course from metaphase to late anaphase the nucleolus organizing secondary constriction was always seen as a heavily impregnated region and the electron density of the cytoplasm increased, probably due to dispersed nucleolar material. An argyrophilic substance began to accumulate on chromosomes in late anaphase. In the beginning of telophase a uniformly impregnated nucleolus was formed at the secondary constriction. It is concluded from these results that argyrophilic substance is associated with RNA-containing structures rather than DNA-containing structures. The silver staining technique presented here is very convenient and favourable, especially for plant cells, to detect specifically the nucleolus organizing region and to survey the nucleolar material during mitosis at the electron microscopic level.

scholarly journals High resolution neurochemical gold staining method for myelin in peripheral and central nervous system at the light- and electron-microscopic level

2009 ◽  
Vol 337 (2) ◽  
pp. 213-221 ◽  
Author(s):  
Nic E. Savaskan ◽  
Oliver Weinmann ◽  
Bernd Heimrich ◽  
Ilker Y. Eyupoglu
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
High Resolution ◽  
Staining Method ◽  
Microscopic Level ◽  
Electron Microscopic Level ◽  
Electron Microscopic

scholarly journals A specific ultrastructural method to reveal DNA: the NAMA-Ur.

1991 ◽  
Vol 39 (10) ◽  
pp. 1427-1438 ◽  
Author(s):  
P S Testillano ◽  
M A Sanchez-Pina ◽  
A Olmedilla ◽  
M A Ollacarizqueta ◽  
C J Tandler ◽  
...  
Keyword(s):  
Ultrastructural Level ◽  
Microscopic Level ◽  
Electron Microscopic Level ◽  
Electron Microscopic ◽  
En Bloc ◽  
Easy Method ◽  
Transmission Electron ◽  
Pre Treatment ◽  
Alkali Hydrolysis

We have developed a new, simple, and reproducible cytochemical method to specifically stain DNA at the electron microscopic level: the NAMA-Ur. It is based on the extraction of RNA and phosphate groups from phosphoproteins by a weak alkali hydrolysis (NA) which does not affect DNA, followed by blockage of the amino and carboxyl groups by methylation and acetylation (MA). Finally, sections are stained by uranyl (Ur), which can bind only to DNA. The efficiency of the pre-treatment (NA and MA) was demonstrated by X-ray microanalysis at the transmission electron microscopic level. The NAMA-Ur method has been successfully performed en bloc and on Lowicryl sections in mammalian and plant cells. A specific contrast is observed in the DNA-containing structures after this method, whose sensitivity allows visualization of electron-dense chromatin fibers of 10-12 nm composed of 3-nm DNA fibrils. This staining method has been combined with anti-DNA antibodies, providing complementary information to detect DNA in situ. We propose the NAMA-Ur as an easy method to investigate the chromatin organization in situ at the ultrastructural level.

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