In Utero Is Superior to Ex Utero Cord Blood Collection.

Abstract Cord blood (CB) cells are being used increasingly as a source of hematopoietic support in patients lacking human leukocyte antigent (HLA)-matched family or unrelated donors. 1124 CB units were collected, based on obstetrician preference, with the placenta either in utero or ex utero. If logistically feasible, both an in utero collection followed immediately by an additional ex utero collection once the placenta was delivered was performed. We compared the distribution of the collection parameters shown in the following table, using non-parametric tests [data expressed as median (range)]: Athough the volume of CB collected was highest with the sequential in utero plus ex utero method, the number of total nucleated cells (TNCs) and CD34+ cells was similar for both the in utero procedures and higher than with the ex utero alone procedure. The number of CB units that had microbial contamination was similar for all three groups: ex utero-0 CB units, in utero-1 CB unit and in utero plus ex utero-1 CB unit. There were no clinical adverse events associated with any of the collection procedures. Conclusion: In utero collections are safe and result in CB units with significantly higher volumes, TNCs and CD34+ cells than ex utero collections, with no difference in the microbial contamination rate. CB Parameters Ex Utero (N=390) In Utero (N=334) In+Ex Utero (N=400) In+Ex vs Ex (p) In+Ex vs In (p) In vs Ex (p) Volume (ml) 76.5 88.5 94 <0.0001 0.03 <0.001 (35–199) (45–185) (42–207) Tot Nucleated cells (xE7) 94 115 119 <0.001 0.4 <0.001 (20–267) (36–399) (41–393) CD34+cells (xE6) 2.9 3.4 3.1 0.2 0.16 0.01 (0–30) (0–34) (0–55)

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Evaluation of Microbial Contamination Rates in Umbilical Cord Blood Collection and Processing for Biobanking in Vietnam

VNU Journal of Science Natural Sciences and Technology ◽

10.25073/2588-1140/vnunst.5300 ◽

2021 ◽

Vol 37 (3) ◽

Author(s):

Chu Thi Thao ◽

Nguyen Van Tinh ◽

Le Cong Luc ◽

Luong Thi Thanh Ha ◽

Tran Van Phuc ◽

...

Keyword(s):

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Microbial Contamination ◽

Contamination Rate ◽

Blood Collection ◽

Delivery Method ◽

Hematopoietic Stem ◽

Cord Blood Collection ◽

High Level

The umbilical cord blood (CB) has recently been considered an abundant source of hematopoietic stem cells (HSCs) for transplantation compared to bone marrow. However, the collection and processing of CB have a high risk of microbial contamination. Hence, the procedures to collect and process the UCB are carefully considered. This study evaluates the microbial contamination rate to find the frequency and distribution of bacterial organisms among CB sampling and processing in Vietnam. In addition, this study correlates with contamination rates between the delivery method, cesarean section, and vaginal delivery. The results create best practices to avoid a high level of contamination of UCB during collection and processing for biobanking.

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In utero or ex utero cord blood collection: an unresolved question

Transfusion ◽

10.1046/j.1537-2995.2003.00459.x ◽

2003 ◽

Vol 43 (8) ◽

pp. 1174-1176 ◽

Cited By ~ 1

Author(s):

Pilar Solves ◽

Rosa Moraga ◽

Vicente Mirabet ◽

Luis Larrea ◽

Ma Angeles Soler

Keyword(s):

Cord Blood ◽

In Utero ◽

Blood Collection ◽

Unresolved Question ◽

Cord Blood Collection

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Transfuse Neonates with Cord Blood-Derived Red Blood Cells: A Feasibility Study to Assess Allogeneic Cord Blood Unit Fractionation and Validation

Blood ◽

10.1182/blood.v120.21.275.275 ◽

2012 ◽

Vol 120 (21) ◽

pp. 275-275 ◽

Cited By ~ 1

Author(s):

Maria Bianchi ◽

Luciana Teofili ◽

Carmen Giannantonio ◽

Alessandra Landini ◽

Patrizia Papacci ◽

...

Keyword(s):

Blood Cell ◽

Cord Blood ◽

Red Blood Cell ◽

Microbial Contamination ◽

Blood Bank ◽

Blood Collection ◽

Red Cell ◽

Cord Blood Bank ◽

Cord Blood Collection ◽

Promising Source

Abstract Abstract 275 Newborns are currently transfused with RBCs from adults, which mainly contain adult hemoglobin (HbA). HbA has a lower affinity for oxygen than fetal hemoglobin: therefore adult red cell transfusion could be responsible for increased oxygen increasing the risk of the “oxygen radicals disease of the newborn”. Autologous umbilical cord blood has been suggested as the only alternative source of blood for newborn transfusions. Previous studies, however, demonstrated that autologous cord blood transfusions in newborns are not sufficient to entirely cover the early neonatal blood requests. We reported the preliminary results of our study carried out to assess the feasibility of an allogeneic cord blood (ACB) transfusion program for prematures in terms of preparation and yield of valid ACB red blood cell (RBC) units. ACB units collected at the Cord Blood Bank but not suitable for processing and storage for allogeneic transplant cord blood were evaluated. Eligible criteria for cord blood collection were: more than 37 weeks of gestation, absence of mother's infection or fever 24 hours before the delivery, no stain of the amniotic fluid. ACB units eligible for our study contained more than 60 mL of cord blood, with no clots or hemolysis. We prepared buffy coat–depleted ACB RC units by automated separation (Compomat G4®, Fresenius HemoCare, Germany) in a processing set (Compoflex®). Suspension in SAG-mannitol and post-storage filtration was performed to obtain a leukocyte-depleted red cell unit. Resuspended units were stored for fourteen days after manipulation (2–6°C). Cultures for bacterial contamination were performed immediately after manipulation and after 14 days; biochemical determination (LDH, glucose, lactate, potassium, chloride, sodium, pH and pO2) were performed the day of fractionation (=0) and after 7 and 14 days of storage. Biochemical data were also compared to the same parameters obtained from adult red blood cell concentrates. We collected 76 ACB units. Thirty-three were discharged for insufficient volume or clots. The median collection volume of the 43 remaining units was 92.3 (± 18.3) ml. After fractionation, 43 ACB RC units were obtained with a median volume of 31.2 (± 8.2) ml and a median hematocrit of 59 ± 2%. Microbial contamination was absent in all units after manipulation and after 14 days; viral tests carried out on mother's blood at the time of cord blood collection were negative. Biochemical parameters maintained rather well up to 14 days of storage, but less resistant than adult red cells. Our data highlight that ACB is a promising source of RBC for transfusion in preterm infants. Besides the reduction of waste of not validated ACB units collected in the Cord Blood Bank, transfusional utilization of ACB RBC can overcome several problems of autologous cord blood transfusion: insufficient volumes is less frequent in ACB from term newborns and the incidence of clots, which is one of the more frequent cause of ineligibility of cord blood units, is substantially reduced when collection is performed by trained staff, in term neonates and using adequate blood shakers. Microbial contamination is prevented by adopting the strict eligibility criteria and the adequate aseptic collection technique adopted in the Cord Blood Bank. In conclusion, the preparation of transfusionally valid RCs from ACB is possible and convenient. Clinical studies are needed to evaluate the efficacy and safety of this new transfusion practice. Disclosures: No relevant conflicts of interest to declare.

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