scholarly journals A myeloid-related sequence that localizes to human chromosome 8q21.1-22

Blood ◽  
1988 ◽  
Vol 71 (6) ◽  
pp. 1713-1719 ◽  
Author(s):  
WM Mars ◽  
P van Tuinen ◽  
HA Drabkin ◽  
JW White ◽  
GF Saunders
Keyword(s):  
Amino Acid ◽  
Human Chromosome ◽  
Somatic Cell ◽  
Southern Blot ◽  
Cell Hybrid ◽  
Somatic Cell Hybrid ◽  
Chromosome 8 ◽  
Nucleotide Sequence Analysis ◽  
Related Sequence ◽  
Hybrid Dnas

Abstract A myeloid-related sequence (mrs) has previously been identified that is highly expressed in selected subpopulations of myeloid leukocytes. Nucleotide sequence analysis indicates that mrs encodes what is apparently a unique 93-amino acid protein that includes an 18-amino acid leader sequence. Hybridization of an mrs cDNA probe to a Southern blot made from somatic cell hybrid DNAs shows 100% concordance with human chromosome 8, thus indicating that mrs localizes to this chromosome. In situ hybridization to metaphase chromosomes further sublocalizes mrs to bands 8q21.1–23 as 58% of the grains displayed on chromosome 8 were clustered in this region. This area encompasses the translocation breakpoint 8q22, which is rearranged in an estimated 18% of patients diagnosed with the M2 subclassification of acute nonlymphocytic leukemia (M2-ANLL). When Southern blot hybridization was performed by using somatic cell hybrid DNAs harboring either a single 8q- or a single 21q+ chromosome from two different patients with M2- ANLL, a signal was only detected in the hybrid containing the 8q- chromosome.

scholarly journals A myeloid-related sequence that localizes to human chromosome 8q21.1-22

Blood ◽  
1988 ◽  
Vol 71 (6) ◽  
pp. 1713-1719
Author(s):  
WM Mars ◽  
P van Tuinen ◽  
HA Drabkin ◽  
JW White ◽  
GF Saunders
Keyword(s):  
Amino Acid ◽  
Human Chromosome ◽  
Somatic Cell ◽  
Southern Blot ◽  
Cell Hybrid ◽  
Somatic Cell Hybrid ◽  
Chromosome 8 ◽  
Nucleotide Sequence Analysis ◽  
Related Sequence ◽  
Hybrid Dnas

A myeloid-related sequence (mrs) has previously been identified that is highly expressed in selected subpopulations of myeloid leukocytes. Nucleotide sequence analysis indicates that mrs encodes what is apparently a unique 93-amino acid protein that includes an 18-amino acid leader sequence. Hybridization of an mrs cDNA probe to a Southern blot made from somatic cell hybrid DNAs shows 100% concordance with human chromosome 8, thus indicating that mrs localizes to this chromosome. In situ hybridization to metaphase chromosomes further sublocalizes mrs to bands 8q21.1–23 as 58% of the grains displayed on chromosome 8 were clustered in this region. This area encompasses the translocation breakpoint 8q22, which is rearranged in an estimated 18% of patients diagnosed with the M2 subclassification of acute nonlymphocytic leukemia (M2-ANLL). When Southern blot hybridization was performed by using somatic cell hybrid DNAs harboring either a single 8q- or a single 21q+ chromosome from two different patients with M2- ANLL, a signal was only detected in the hybrid containing the 8q- chromosome.

Somatic Cell Hybrid Panel and NotI Linking Clones for Physical Mapping of Human Chromosome 3

Genomics ◽  
1994 ◽  
Vol 20 (1) ◽  
pp. 105-113 ◽  
Author(s):  
Ji-Yi Wang ◽  
Eugene R. Zabarovsky ◽  
Cathy Talmadge ◽  
Peter Berglund ◽  
Kate W.K. Chan ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Somatic Cell ◽  
Physical Mapping ◽  
Cell Hybrid ◽  
Somatic Cell Hybrid ◽  
Chromosome 3 ◽  
Somatic Cell Hybrid Panel ◽  
Human Chromosome 3

scholarly journals A somatic cell hybrid panel and DNA probes for physical mapping of human chromosome 7p

Genomics ◽  
1991 ◽  
Vol 11 (3) ◽  
pp. 737-743 ◽  
Author(s):  
A. Vortkamp ◽  
U. Thias ◽  
M. Gessler ◽  
W. Rosenkranz ◽  
P.M. Kroisel ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Somatic Cell ◽  
Physical Mapping ◽  
Cell Hybrid ◽  
Somatic Cell Hybrid ◽  
Dna Probes ◽  
Somatic Cell Hybrid Panel

Construction and Screening of a Cosmid Library Generated from a Somatic Cell Hybrid Bearing Human Chromosome 15

Genomics ◽  
1997 ◽  
Vol 40 (1) ◽  
pp. 63-72
Author(s):  
Lisa D. McDaniel ◽  
Bingqing Zhang ◽  
Ewa Kubiczek ◽  
Marisa Ritter ◽  
Jing Huang ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Somatic Cell ◽  
Cell Hybrid ◽  
Somatic Cell Hybrid ◽  
Cosmid Library ◽  
Chromosome 15 ◽  
Hybrid Bearing

scholarly journals Localization of the casein gene family to a single mouse chromosome.

1982 ◽  
Vol 93 (1) ◽  
pp. 199-204 ◽  
Author(s):  
P Gupta ◽  
J M Rosen ◽  
P D'Eustachio ◽  
F H Ruddle
Keyword(s):  
Gene Family ◽  
Somatic Cell ◽  
Mouse Chromosome ◽  
Cell Hybrid ◽  
Somatic Cell Hybrid ◽  
Mammary Tissue ◽  
Cdna Clones ◽  
Chromosome 5 ◽  
Casein Gene ◽  
Hybrid Dnas

A series of mouse-hamster somatic cell hybrids containing a variable number of mouse chromosomes and a constant set of hamster chromosomes have been used to determine the chromosomal location of a family of hormone-inducible genes, the murine caseins. Recombinant mouse cDNA clones encoding the alpha-, beta-, and gamma-caseins were constructed and used in DNA restriction mapping experiments. All three casein cDNAs hybridized to the same set of somatic cell hybrid DNAs isolated from cells containing mouse chromosome 5, while negative hybridization was observed to ten other hybrid DNAs isolated from cells lacking chromosome 5. A fourth cDNA clone, designated pCM delta 40, which hybridized to an abundant 790 nucleotide poly(A)RNA isolated from 6-d lactating mouse mammary tissue, was also mapped to chromosome 5. The chromosomal assignment of the casein gene family was confirmed using a mouse albumin clone. The albumin gene had been previously localized to mouse chromosome 5 by both breeding studies and analogous molecular hybridization experiments. An additional control experiment demonstrated that another hormone-inducible gene, specifying a 620 nucleotide abundant mammary gland mRNA, hybridized to DNA isolated from a different somatic cell hybrid line. These studies represent the first localization of a peptide and steroid hormone-responsive gene family to a single mouse chromosome.

Regional Localization of over 300 Loci on Human Chromosome 22 Using a Somatic Cell Hybrid Mapping Panel

Genomics ◽  
1996 ◽  
Vol 35 (2) ◽  
pp. 275-288 ◽  
Author(s):  
Marcia L. Budarf ◽  
Barbara Eckman ◽  
Dominique Michaud ◽  
Terrence McDonald ◽  
Stacey Gavigan ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Somatic Cell ◽  
Cell Hybrid ◽  
Somatic Cell Hybrid ◽  
Chromosome 22 ◽  
Hybrid Mapping ◽  
Regional Localization ◽  
Mapping Panel
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