scholarly journals Multiplex quantitative PCR for detection of lower respiratory tract infection and meningitis caused by Streptococcus pneumoniae, Haemophilus influenzae and Neisseria meningitidis

2010 ◽  
Vol 10 (1) ◽  
pp. 310 ◽  
Author(s):  
Guma MK Abdeldaim ◽  
Kristoffer Strålin ◽  
Jens Korsgaard ◽  
Jonas Blomberg ◽  
Christina Welinder-Olsson ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Tract Infection ◽  
Respiratory Tract ◽  
Haemophilus Influenzae ◽  
Neisseria Meningitidis ◽  
Respiratory Tract Infection ◽  
Lower Respiratory Tract Infection ◽  
Quantitative Pcr ◽  
Lower Respiratory Tract

scholarly journals Evaluation of a Rapid Immunochromatographic ODK0501 Assay for Detecting Streptococcus pneumoniae Antigen in Sputum Samples from Patients with Lower Respiratory Tract Infection

2009 ◽  
Vol 16 (5) ◽  
pp. 672-678 ◽  
Author(s):  
Koichi Izumikawa ◽  
Suguru Akamatsu ◽  
Akiko Kageyama ◽  
Kiyomi Okada ◽  
Yukumasa Kazuyama ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Tract Infection ◽  
Respiratory Tract ◽  
Respiratory Tract Infection ◽  
Real Time ◽  
Lower Respiratory Tract Infection ◽  
Real Time Pcr ◽  
Lower Respiratory Tract ◽  
Bacterial Culture ◽  
Antigen Test

ABSTRACT A novel, rapid, and noninvasive test (ODK0501) to detect Streptococcus pneumoniae antigen was evaluated in a Japanese multicenter study. ODK0501 uses polyclonal antibodies to detect C polysaccharide of S. pneumoniae from sputum samples by an immunochromatographic assay. The utility of ODK0501 was evaluated for 161 adult patients with lower respiratory tract infection between March 2006 and March 2007. Bacterial culture and identification, real-time PCR, and ODK0501 assays were performed on sputum samples, and the Binax Now Streptococcus pneumoniae antigen test was performed using urine samples obtained from the same patients. The performances of all tests were compared based on the results of bacterial culture and identification. The sensitivity and specificity of ODK0501 were 89.1% (49/55 samples) and 95.3% (101/106 samples), respectively. We then compared the Binax Now Streptococcus pneumoniae antigen test with ODK0501 using samples from 142 patients. The sensitivities of ODK0501 and the Binax Now S. pneumoniae antigen test were 90.0% (45/50 samples) and 62.0% (31/50 samples), respectively (P = 0.002). The relative quantity of S. pneumoniae in expectorated sputum was calculated using real-time PCR and indicated that the possibility of false-positive results for ODK0501 due to indigenous S. pneumoniae was low. The positive and negative concordance rates of ODK0501 and Binax Now were 96.8% (30/31 samples) and 21.1% (4/19 samples), respectively. Binax Now was less capable of detecting S. pneumoniae antigen among patients with underlying chronic obstructive pulmonary disease. In conclusion, ODK0501 is noninvasive, rapid, and an accurate tool for diagnosing respiratory infection caused by S. pneumoniae.

scholarly journals Characteristics and Antibiotic Resistance of Haemophilus influenzae in Children with Lower Respiratory Tract Infection in Chengdu, China

2021 ◽  
Vol 14 (2) ◽  
Author(s):  
Qin Wang ◽  
Cheng-Gui Liu ◽  
Jian Xu ◽  
Qin Zhang ◽  
Chong-Hui Zhao ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Tract Infection ◽  
Respiratory Tract ◽  
Haemophilus Influenzae ◽  
Respiratory Tract Infection ◽  
Lower Respiratory Tract Infection ◽  
Lower Respiratory Tract ◽  
Detection Rate ◽  
Human Respiratory Tract ◽  
Positive Rate

Background: Haemophilus influenzae is an opportunistic pathogen of the human respiratory tract. Haemophilus influenzae can cause not only respiratory tract infection in children but also otitis media, epiglottitis and sinusitis. With the widespread use of antibiotics, the positive rate of β-lactamase in H. influenzae is increasing, and the rate of antimicrobial resistance is also increasing, which increases the difficulty of clinical treatment. Objectives: To study the infection characteristics of patients and the antibiotic resistance of H. influenzae in lower respiratory tract samples of children in Chengdu, so as to provide a reference for its clinical diagnosis and the rational use of antibiotics. Methods: Sputum samples of 15891 children aged 0-14 years with lower respiratory tract infection were collected. Haemophilus influenzae was cultured and identified, its drug susceptibility tested, and the results determined according to the guidelines of CLSI 2020. Results: A total of 15891 clinical isolate strains in sputum were detected for drug sensitivity from December 2018 to January 2020, of which 5488 were H. influenzae, accounting for 34.54% (5488/15891). The sex of children infected with H. influenzae was not skewed (P > 0.05). The detection rate of H. influenzae was the highest in children aged 7 - 11 months, and the lowest was in the age group ≤ 28 d. The detection rate was the highest in spring and the lowest in autumn. The positive rate of β-lactamase was 92.0%, the resistance rate to ampicillin was 92.0%, the sensitivity to amoxicillin/clavulanate was 70.2%, and the sensitivity to cefotaxime, ofloxacin, tetracycline, chloramphenicol, and rifampicin was more than 90.0%. Conclusions: Children aged 7 months to 14 years were generally susceptible to H. influenzae in spring, and the positive rate of β-lactamase was high. Doctors should refer to the infection characteristics and drug resistance of H. influenzae and choose antibiotics correctly to better control the infection.

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