scholarly journals A one-step cloning method for the construction of somatic cell gene targeting vectors: application to production of human knockout cell lines

2012 ◽  
Vol 12 (1) ◽  
pp. 71 ◽  
Author(s):  
Yi Liu ◽  
Shangze Li ◽  
Huihui Zhang ◽  
Zurong Wan ◽  
Xiaodong Zhang ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Gene Targeting ◽  
Cell Lines ◽  
Cloning Method ◽  
One Step ◽  
Cell Gene

Human Somatic Cell Gene Targeting

2003 ◽  
Vol 62 (1) ◽  
Author(s):  
Todd Waldman ◽  
Carolyn Lee ◽  
Tagvor G. Nishanian ◽  
Jung‐Sik Kim
Keyword(s):  
Somatic Cell ◽  
Gene Targeting ◽  
Human Somatic Cell ◽  
Cell Gene

scholarly journals Simple Monitoring of Gene Targeting Efficiency in Human Somatic Cell Lines Using the PIGA Gene

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e47389 ◽  
Author(s):  
Sivasundaram Karnan ◽  
Yuko Konishi ◽  
Akinobu Ota ◽  
Miyuki Takahashi ◽  
Lkhagvasuren Damdindorj ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Gene Targeting ◽  
Cell Lines ◽  
Human Somatic Cell ◽  
Targeting Efficiency

A PCR-based high-throughput screen with multiround sample pooling: application to somatic cell gene targeting

2007 ◽  
Vol 2 (11) ◽  
pp. 2865-2874 ◽  
Author(s):  
Hiroyuki Konishi ◽  
Josh Lauring ◽  
Joseph P Garay ◽  
Bedri Karakas ◽  
Abde M Abukhdeir ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Gene Targeting ◽  
High Throughput ◽  
High Throughput Screen ◽  
Sample Pooling ◽  
Cell Gene

scholarly journals Production of CFTR-null and CFTR-ΔF508 heterozygous pigs by adeno-associated virus–mediated gene targeting and somatic cell nuclear transfer

2008 ◽  
Vol 118 (4) ◽  
pp. 1571-1577 ◽  
Author(s):  
Christopher S. Rogers ◽  
Yanhong Hao ◽  
Tatiana Rokhlina ◽  
Melissa Samuel ◽  
David A. Stoltz ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Gene Targeting ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Adeno Associated Virus

CHROMOSOMES AND DNA OF MICROTUS II. CONFIRMATION OF DELETION OF CONSTITUTIVE HETEROCHROMATIN IN M. AGRESTIS CELLS IN VITRO

1977 ◽  
Vol 19 (3) ◽  
pp. 537-541 ◽  
Author(s):  
J. E. K. Cooper
Keyword(s):  
Somatic Cell ◽  
Cell Line ◽  
Cell Lines ◽  
X Chromosome ◽  
Sex Chromosomes ◽  
Constitutive Heterochromatin ◽  
The Other ◽  
Microtus Agrestis ◽  
C Banding

The distribution of constitutive heterochromatin has been examined by C-banding in two somatic cell lines, grown in vitro, from a female Microtus agrestis. One line retains one intact X chromosome together with the short arm of the other X chromosome, while the other cell line retains only the short arm of one X chromosome. Thus, each cell line has lost substantial amounts of heterochromatin from the sex chromosomes, but this material has been deleted from the cells, and not translocated to other chromosomes. Nonetheless, both cell lines continue to propagate well in vitro.

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