scholarly journals Functional characterization of an arrestin gene on insecticide resistance of Culex pipiens pallens

2012 ◽  
Vol 5 (1) ◽  
pp. 134 ◽  
Author(s):  
Yan Sun ◽  
Ping Zou ◽  
Xin-You Yu ◽  
Chen Chen ◽  
Jing Yu ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Culex Pipiens ◽  
Functional Characterization ◽  
Culex Pipiens Pallens ◽  
Pipiens Pallens

scholarly journals Cloning and characterization of prophenoloxidase A3 (proPOA3) from Culex pipiens pallens

2012 ◽  
Vol 162 (4) ◽  
pp. 57-65 ◽  
Author(s):  
Dan Zhou ◽  
Shunhua Hao ◽  
Yan Sun ◽  
Lin Chen ◽  
Chunrong Xiong ◽  
...  
Keyword(s):  
Culex Pipiens ◽  
Culex Pipiens Pallens ◽  
Pipiens Pallens

scholarly journals Isolation and characterization of the full coding sequence of a novel densovirus from the mosquito Culex pipiens pallens

2008 ◽  
Vol 89 (1) ◽  
pp. 195-199 ◽  
Author(s):  
You-gang Zhai ◽  
Xin-jun Lv ◽  
Xiao-hong Sun ◽  
Shi-hong Fu ◽  
Zheng-da Gong ◽  
...  
Keyword(s):  
Culex Pipiens ◽  
Phylogenetic Trees ◽  
Open Reading Frames ◽  
Coding Region ◽  
Culex Pipiens Pallens ◽  
Virus Particles ◽  
Isolation And Characterization ◽  
Novel Variant ◽  
Pipiens Pallens

During an investigation of arboviruses in China, a novel densovirus (DNV) was isolated from the adult female Culex pipiens pallens. The virus, designated Culex pipiens pallens densovirus (CppDNV), caused cytopathic effect in C6/36 cells. The virus particles were icosahedral, non-enveloped and had a mean diameter of 24 nm. The complete coding region of CppDNV was found to be 3335 nt and it contained three open reading frames (ORFs). CppDNV shares 82–93 % identical nucleotides with isolates of the Aedes albopictus densovirus [isolates AalDNV-1, AalDNV-2 (C6/36 DNV) and AalDNV-3], Aedes aegypti densovirus (AaeDNV) and Haemagogus equines densovirus (HeDNV). The nucleotide sequence identity among CppDNV isolates exceeds 98 %. Phylogenetic trees based on non-structural (NS1 and NS2) and capsid (VP) genes show that CppDNV clustered with the species AaeDNV and represents a novel variant of this species within the genus Brevidensovirus.

scholarly journals MiR-279-3p regulates deltamethrin resistance through CYP325BB1 in Culex pipiens pallens

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Xixi Li ◽  
Shengli Hu ◽  
Hongbo Zhang ◽  
Haitao Yin ◽  
Huan Wang ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Culex Pipiens ◽  
Oral Feeding ◽  
Luciferase Reporter ◽  
Culex Pipiens Pallens ◽  
Mosquito Mortality ◽  
Deltamethrin Resistance ◽  
Pipiens Pallens

Abstract Background The overuse of insecticides to control insect vectors has promoted extensive insecticide resistance in mosquitoes. In this study, the functions of microRNA (miR)-279-3p and its target CYP325BB1 in the regulation of deltamethrin resistance in Culex pipiens pallens was investigated. Methods Quantitative real-time reverse transcription PCR was used to detect the expression levels of miR-279-3p and CYP325BB1. Then, the dual-luciferase reporter assay system, RNA interference, CDC bottle bioassay and Cell Counting Kit-8 (CCK-8) assay were used to explore the roles of these molecules in deltamethrin resistance both in vivo and in vitro. Results The expression patterns of miR-279-3p and CYP325BB1 were compared between deltamethrin-sensitive (DS-strain) and deltamethrin-resistant (DR-strain) mosquitoes. Luciferase activity was downregulated by miR-279-3p, the effect of which was ablated by a mutation of the putative binding site for CYP325BB1. In DR-strain mosquitoes, the expression of miR-279-3p was increased by microinjection and oral feeding of miR-279-3p agomir (mimic). CYP325BB1 mRNA levels were downregulated, which resulted in a higher mortality of the mosquitoes in miR-279-3p mimic-treated groups. In the DS-strain mosquitoes, microinjection of a miR-279-3p inhibitor decreased miR-279-3p expression, whereas the expression of CYP325BB1 was increased; the mortality of these mosquitoes decreased significantly. In addition, overexpression of pIB/V5-His-CYP325BB1 changed the sensitivity of C6/36 cells to deltamethrin in vitro. Also in DR-strain mosquitoes, downregulation of CYP325BB1 expression by microinjection of si-CYP325BB1 increased mosquito mortality in vivo. Conclusions These findings provide empirical evidence of the involvement of miRNAs in the regulation of insecticide resistance and indicate that miR-279-3p suppresses the expression of CYP325BB1, which in turn decreases deltamethrin resistance, resulting in increased mosquito mortality. Taken together, the results provide important information for use in the development of future mosquito control strategies. Graphical abstract

scholarly journals Cloning and characterization of NYD-OP7, a novel deltamethrin resistance associated gene from Culex pipiens pallens

2007 ◽  
Vol 88 (1) ◽  
pp. 82-91 ◽  
Author(s):  
Xiaobang Hu ◽  
Yan Sun ◽  
Weijie Wang ◽  
Mingxia Yang ◽  
Lixin Sun ◽  
...  
Keyword(s):  
Culex Pipiens ◽  
Culex Pipiens Pallens ◽  
Deltamethrin Resistance ◽  
Pipiens Pallens

scholarly journals Comparative proteomics reveals mechanisms that underlie insecticide resistance in Culex pipiens pallens Coquillett

2021 ◽  
Vol 15 (3) ◽  
pp. e0009237
Author(s):  
Chongxing Zhang ◽  
Qiqi Shi ◽  
Tao Li ◽  
Peng Cheng ◽  
Xiuxia Guo ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Insecticide Resistance ◽  
Culex Pipiens ◽  
Mosquito Control ◽  
Expression Profiles ◽  
Susceptible Strain ◽  
Culex Pipiens Pallens ◽  
Metabolic Resistance ◽  
Tandem Mass Spectrometric ◽  
Pipiens Pallens

Mosquito control based on chemical insecticides is considered as an important element of the current global strategies for the control of mosquito-borne diseases. Unfortunately, the development of insecticide resistance of important vector mosquito species jeopardizes the effectiveness of insecticide-based mosquito control. In contrast to target site resistance, other mechanisms are far from being fully understood. Global protein profiles among cypermethrin-resistant, propoxur-resistant, dimethyl-dichloro-vinyl-phosphate-resistant and susceptible strain of Culex pipiens pallens were obtained and proteomic differences were evaluated by using isobaric tags for relative and absolute quantification labeling coupled with liquid chromatography/tandem mass spectrometric analysis. A susceptible strain of Culex pipiens pallens showed elevated resistance levels after 25 generations of insecticide selection, through iTRAQ data analysis detected 2,502 proteins, of which 1,513 were differentially expressed in insecticide-selected strains compared to the susceptible strain. Finally, midgut differential protein expression profiles were analyzed, and 62 proteins were selected for verification of differential expression using iTRAQ and parallel reaction monitoring strategy, respectively. iTRAQ profiles of adaptation selection to three insecticide strains combined with midgut profiles revealed that multiple insecticide resistance mechanisms operate simultaneously in resistant insects of Culex pipiens pallens. Significant molecular resources were developed for Culex pipiens pallens, potential candidates were involved in metabolic resistance and reducing penetration or sequestering insecticide. Future research that is targeted towards RNA interference of the identified metabolic targets, such as cuticular proteins, cytochrome P450s, glutathione S-transferases and ribosomal proteins proteins and biological pathways (drug metabolism—cytochrome P450, metabolism of xenobiotics by cytochrome P450, oxidative phosphorylation, ribosome) could lay the foundation for a better understanding of the genetic basis of insecticide resistance in Culex pipiens pallens.

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