scholarly journals Nuclear and Cytoplasmic Maturation of Bovine Oocytes Cultured with dbc AMP, FSH AND hCG

1986 ◽  
Vol 27 (4) ◽  
pp. 566-574
Author(s):  
Pavel Liehman ◽  
Torben Greve ◽  
Rang Pu Xu
Keyword(s):  
Cytoplasmic Maturation ◽  
Bovine Oocytes

Effects of different kinases and phosphatases on nuclear and cytoplasmic maturation of bovine oocytes

1995 ◽  
Vol 42 (1) ◽  
pp. 114-121 ◽  
Author(s):  
Johanne T. Léavesque ◽  
Marc-André Sirard
Keyword(s):  
Cytoplasmic Maturation ◽  
Bovine Oocytes

Erratum to Cytoplasmic maturation of bovine oocytes: Structural and biochemical modifications and acquisition of developmental competence

2010 ◽  
Vol 73 (8) ◽  
pp. 1164
Author(s):  
E.M. Ferreira ◽  
A.A. Vireque ◽  
P.R. Adona ◽  
F.V. Meirelles ◽  
R.A. Ferriani ◽  
...  
Keyword(s):  
Developmental Competence ◽  
Cytoplasmic Maturation ◽  
Bovine Oocytes

Vitrification of Bovine Oocytes: Effect of the Meiotic Stage on Nuclear and Cytoplasmic Maturation.

2011 ◽  
Vol 85 (Suppl_1) ◽  
pp. 721-721
Author(s):  
Jose Spricigo ◽  
Rodolfo Rumpf ◽  
Margot A.N. Dode
Keyword(s):  
Meiotic Stage ◽  
Cytoplasmic Maturation ◽  
Bovine Oocytes

Role of roscovitine and IBMX on kinetics of nuclear and cytoplasmic maturation of bovine oocytes in vitro

2007 ◽  
Vol 99 (1-2) ◽  
pp. 202-207 ◽  
Author(s):  
L.S.S. Barretto ◽  
V.S.D. Caiado Castro ◽  
J.M. Garcia ◽  
G.Z. Mingoti
Keyword(s):  
Cytoplasmic Maturation ◽  
Bovine Oocytes ◽  
Kinetics Of

scholarly journals Oxygen tension and oocyte density during in vitro maturation affect the in vitro fertilization of bovine oocytes

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4277 ◽  
Author(s):  
Angelo Bertani Giotto ◽  
Daniela Dos Santos Brum ◽  
Francielli Weber Santos ◽  
Antonio Carlos Galarça Guimarães ◽  
Cibele Garcia Moreira Gonçalves ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Oxygen Tension ◽  
Polar Body ◽  
Ros Production ◽  
Low Oxygen ◽  
Nuclear Maturation ◽  
First Polar Body ◽  
Cytoplasmic Maturation ◽  
Bovine Oocytes

<p>Oocyte maturation is the key factor affecting the fertilization and embryonic development. Factors such as oocyte density and oxygen tension can directly influence the IMV. Thus, the objective of this study was to evaluate the effect of the association of oxygen tensions (5% or 20%) with different oocyte densities (1:10?l or 1:20?l) in the <em>in vitro </em>maturation (IVM) of bovine oocytes on maturation and fertilization rates, ROS production and antioxidant activity. Three experiments were performed with bovine oocytes that were obtained from slaughterhouse ovaries. After selection, the oocytes were randomly distributed in four treatments: 1:10/5%; 1:10/20%; 1:20/5%and 1:20/20% for each experiment. In experiment I, nuclear maturation status and cytoplasmic maturation were evaluated through detection of the first polar body by immunofluorescence and the mitochondrial reorganization assay. In experiment II, ROS production and antioxidant activity were analyzed in oocytes and IVM medium after 24 h of maturation through detection of ROS, reduced glutathione (GSH) and Superoxide dismutase activity by spectrofluorimetric methods. In experiment III, fertilization was evaluated through pronucleus formation, sperm penetration with or without decondensation and polyspermy rates by immunofluorescence. In experiment I, the nuclear maturation and cytoplasmic maturation were similar among treatments (P&gt;0.05). In experiment II, reactive oxygen species in oocytes were elevated in treatments with low oxygen tension which was independent of oocyte density (P&lt;0.05). Additionally, ROS levels in IVM medium were higher in treatments with high oocyte density by volume of medium, which was independent of oxygen tension (P&lt;0.05). In Experiment III, the fertilization and penetration rates were higher in the treatment with 20% oxygen tension and high oocyte density (P&lt;0.05). Furthermore, a high incidence of polyspermy was observed in groups with high oxygen tension and low oocyte density (P&lt;0.05). In conclusion, the results of this study indicate an interaction between oxygen tension and oocyte density, which increases ROS production in certain associations and subsequently influences the rates of <em>in vitro </em>fertilization of bovine oocytes. The improved rates of IVF were obtained when IVM was conducted using 20% oxygen tension and high oocyte density (1:20 ul).</p>

Effect of follicle size and quality on the ability of follicular fluid to support cytoplasmic maturation of bovine oocytes

1995 ◽  
Vol 43 (1) ◽  
pp. 182
Author(s):  
C. Carolan ◽  
P. Lonergan ◽  
P. Monget ◽  
D. Monniaux ◽  
P. Mermillod
Keyword(s):  
Follicular Fluid ◽  
Follicle Size ◽  
Cytoplasmic Maturation ◽  
Bovine Oocytes

scholarly journals Improving the cytoplasmic maturation of bovine oocytes matured in vitro with intracellular and/or extracellular antioxidants is not associated with increased rates of embryo development

2016 ◽  
Vol 86 (8) ◽  
pp. 1897-1905 ◽  
Author(s):  
Nathália A.S. Rocha-Frigoni ◽  
Beatriz C.S. Leão ◽  
Priscila Chediek Dall'Acqua ◽  
Gisele Z. Mingoti
Keyword(s):  
Embryo Development ◽  
Cytoplasmic Maturation ◽  
Bovine Oocytes

Cytoplasmic maturation of bovine oocytes: Structural and biochemical modifications and acquisition of developmental competence

2009 ◽  
Vol 71 (5) ◽  
pp. 836-848 ◽  
Author(s):  
E.M. Ferreira ◽  
A.A. Vireque ◽  
P.R. Adona ◽  
F.V. Meirelles ◽  
R.A. Ferriani ◽  
...  
Keyword(s):  
Developmental Competence ◽  
Cytoplasmic Maturation ◽  
Bovine Oocytes

Growth hormone promotes cytoplasmic maturation of in vitro matured bovine oocytes

1998 ◽  
Vol 49 (1) ◽  
pp. 312 ◽  
Author(s):  
F. Izadyar ◽  
W.J. Hage ◽  
B. Colenbrander ◽  
M.M. Bevers
Keyword(s):  
Growth Hormone ◽  
Cytoplasmic Maturation ◽  
Bovine Oocytes

scholarly journals Oxygen tension and oocyte density during in vitro maturation affect the in vitro fertilization of bovine oocytes

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4277
Author(s):  
Angelo Bertani Giotto ◽  
Daniela Dos Santos Brum ◽  
Francielli Weber Santos ◽  
Antonio Carlos Galarça Guimarães ◽  
Cibele Garcia Moreira Gonçalves ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
In Vitro Fertilization ◽  
Oxygen Tension ◽  
In Vitro Maturation ◽  
Ros Production ◽  
Vitro Fertilization ◽  
Nuclear Maturation ◽  
Cytoplasmic Maturation ◽  
Bovine Oocytes

Oocyte maturation is the key factor affecting the fertilization and embryonic development. Factors such as oocyte density and oxygen tension can directly influence the IMV. Thus, the objective of this study was to evaluate the effect of the association of oxygen tensions (5% or 20%) with different oocyte densities (1:10?l or 1:20?l) in the in vitro maturation (IVM) of bovine oocytes on maturation and fertilization rates, ROS production and antioxidant activity. Three experiments were performed with bovine oocytes that were obtained from slaughterhouse ovaries. After selection, the oocytes were randomly distributed in four treatments: 1:10/5%; 1:10/20%; 1:20/5%and 1:20/20% for each experiment. In experiment I, nuclear maturation status and cytoplasmic maturation were evaluated through detection of the first polar body by immunofluorescence and the mitochondrial reorganization assay. In experiment II, ROS production and antioxidant activity were analyzed in oocytes and IVM medium after 24 h of maturation through detection of ROS, reduced glutathione (GSH) and Superoxide dismutase activity by spectrofluorimetric methods. In experiment III, fertilization was evaluated through pronucleus formation, sperm penetration with or without decondensation and polyspermy rates by immunofluorescence. In experiment I, the nuclear maturation and cytoplasmic maturation were similar among treatments (P>0.05). In experiment II, reactive oxygen species in oocytes were elevated in treatments with low oxygen tension which was independent of oocyte density (P<0.05). Additionally, ROS levels in IVM medium were higher in treatments with high oocyte density by volume of medium, which was independent of oxygen tension (P<0.05). In Experiment III, the fertilization and penetration rates were higher in the treatment with 20% oxygen tension and high oocyte density (P<0.05). Furthermore, a high incidence of polyspermy was observed in groups with high oxygen tension and low oocyte density (P<0.05). In conclusion, the results of this study indicate an interaction between oxygen tension and oocyte density, which increases ROS production in certain associations and subsequently influences the rates of in vitro fertilization of bovine oocytes. The improved rates of IVF were obtained when IVM was conducted using 20% oxygen tension and high oocyte density (1:20 ul).

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