scholarly journals Association of mitochondrial respiratory chain enzymes with the risk and mortality of sepsis among Chinese children

2022 ◽  
Vol 22 (1) ◽  
Author(s):  
Danni He ◽  
Ning Li ◽  
Xiuxiu Lu ◽  
Wei Li ◽  
Yuanmei Chen ◽  
...  
Keyword(s):  
Respiratory Chain ◽  
Complex I ◽  
Mitochondrial Respiratory Chain ◽  
Chinese Children ◽  
Healthy Controls ◽  
Respiratory Chain Enzyme ◽  
Pediatric Sepsis ◽  
Fof1 Atpase ◽  
Incident Cases ◽  
Mitochondrial Respiratory

Abstract Background Sepsis is a leading cause of pediatric morbidity and mortality worldwide. The aim of this study was to explore the association of decreased mitochondrial respiratory chain enzyme activities with the risk for pediatric sepsis, and explore their association with mortality among affected children. Methods A total of 50 incident cases with sepsis and 49 healthy controls participated in this study. The level of serum coenzyme Q10 was measured by high-performance liquid chromatography, and selected mitochondrial respiratory chain enzymes in WBC were measured using spectrophotometric. Logistic regression models were used to estimate odds ratio (OR) and 95% confidence interval (CI). Results The levels of CoQ10, complex II, complex I + III and FoF1-ATPase were significantly higher in healthy controls than in children with sepsis (p < 0.001, = 0.004, < 0.001 and < 0.001, respectively). In children with sepsis, levels of CoQ10 and complex I + III were significantly higher in survived cases than in deceased cases (p < 0.001). Per 0.05 μmol/L, 50 nmol/min.mg and 100 nmol/min.mg increment in CoQ10, complex I + III and FoF1-ATPase were associated with significantly lowered risk of having sepsis, even after adjusting for confounding factors (OR = 0.85, 0.68 and 0.04, p = 0.001, < 0.001 and < 0.001, respectively). Per 0.05 μmol/L and 50 nmol/min.mg increment in CoQ10 and complex I + III was associated with significantly lowered risk of dying from sepsis during hospitalization, and significance retained after adjustment (OR = 0.73 and 0.76, 95% CI: 0.59 to 0.90 and 0.64 to 0.89, p = 0.004 and 0.001, respectively) in children with sepsis. Conclusions Our findings indicate the promising predictive contribution of low serum CoQ10 and complex I + III to the risk of pediatric sepsis and its associated mortality during hospitalization among Chinese children. Trial registration The trial was registered with www.chictr.org.cn, number ChiCTR-IOR-15006446 on May 05, 2015. Retrospectively registered.

scholarly journals Association of Mitochondrial Respiratory Chain Enzymes with the Risk and Mortality of Sepsis Among Chinese Children

2020 ◽  
Author(s):  
Danni He ◽  
Ning Li ◽  
Xiuxiu Lu ◽  
Wei Li ◽  
Yuanmei Chen ◽  
...  
Keyword(s):  
Respiratory Chain ◽  
Complex I ◽  
Mitochondrial Respiratory Chain ◽  
Chinese Children ◽  
Respiratory Chain Enzyme ◽  
Logistic Regression Models ◽  
Pediatric Sepsis ◽  
Fof1 Atpase ◽  
Incident Cases ◽  
Mitochondrial Respiratory

Abstract Background: Sepsis is a leading cause of pediatric morbidity and mortality worldwide. The aim of this study was to explore the association of mitochondrial respiratory chain enzyme activities with the risk for pediatric sepsis, and interrogate their association with hospitalized mortality among affected children.Methods: A total of 50 incident cases with sepsis and 49 healthy controls participated in this study. Logistic regression models were used to estimate odds ratio (OR) and 95% confidence interval (CI).Results: The levels of CoQ10, complex II, complex I+III and FoF1-ATPase were significantly higher in controls than in cases. In children with sepsis, levels of CoQ10 and complex I+III were significantly higher in survived cases than in deceased cases. Per 0.05 μmol/L, 50 nmol/min.mg and 100 nmol/min.mg increment in CoQ10, complex I+III and FoF1-ATPase were associated with significantly lowered risk of having sepsis, even after adjusting for confounding factors (OR=0.85, 0.68 and 0.04, P: 0.001, <0.001 and <0.001, respectively). Per 0.05 μmol/L and 50 nmol/min.mg increment in CoQ10 and complex I+III was associated with significantly lowered risk of dying from sepsis during hospitalization, and significance retained after adjustment (OR=0.73 and 0.76, 95% CI: 0.59 to 0.90 and 0.64 to 0.89, P=0.004 and 0.001, respectively) in sepsis children.Conclusion: Our findings indicate the promising predictive contribution of serum CoQ10 and complex I+III to the risk of pediatric sepsis and its associated mortality during hospitalization among Chinese children.

scholarly journals Mitochondrial Disease as a Cause of Neonatal Hemophagocytic Lymphohistiocytosis

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Kazumasa Fuwa ◽  
Mitsuru Kubota ◽  
Masami Kanno ◽  
Hiroshi Miyabayashi ◽  
Ken Kawabata ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Respiratory Chain ◽  
Hemophagocytic Lymphohistiocytosis ◽  
Complex I ◽  
Clinical Course ◽  
Bone Marrow Aspiration ◽  
Mitochondrial Respiratory Chain ◽  
Respiratory Chain Enzyme ◽  
Mitochondrial Respiratory Chain Disorder ◽  
Mitochondrial Respiratory

Diagnosis of mitochondrial respiratory chain disorder (MRCD) is often difficult. Its pathogenesis is still unclear. We diagnosed MRCD by measuring the activity of the mitochondrial respiratory chain enzyme, and the patient also had hemophagocytic lymphohistiocytosis (HLH). A preterm female infant was born at 34 weeks of gestation. On day 6, HLH was revealed by bone marrow aspiration. She died on day 10 due to uncontrollable HLH. An autopsy was performed, and we measured the activity of the mitochondrial respiratory chain enzyme in the liver, muscle, and heart. The activity of complex I was decreased in all tissues. As we could not prove another origin of the HLH, she was diagnosed as having HLH caused by MRCD. It is useful to measure the activity of the mitochondrial respiratory chain enzyme for diagnosing MRCD. MRCD, which has a severe clinical course, may be related to HLH.

scholarly journals Mitochondrial Respiratory Chain Protein Co-Regulation in the Human Brain

2021 ◽  
Author(s):  
Caroline Trumpff ◽  
Edward Owusu-Ansah ◽  
Hans-Ulrich Klein ◽  
Annie Lee ◽  
Vladislav Petyuk ◽  
...  
Keyword(s):  
Human Brain ◽  
Respiratory Chain ◽  
Complex I ◽  
Molecular Mechanisms ◽  
Nuclear Dna ◽  
Mitochondrial Protein ◽  
Mitochondrial Respiratory Chain ◽  
Mtdna Copy Number ◽  
Mt Dna ◽  
Mitochondrial Respiratory

Mitochondrial respiratory chain (RC) function requires the stochiometric interaction among dozens of proteins but their co-regulation has not been defined in the human brain. Here, using quantitative proteomics across three independent cohorts we systematically characterized the co-regulation patterns of mitochondrial RC proteins in the human dorsolateral prefrontal cortex (DLPFC). Whereas the abundance of RC protein subunits that physically assemble into stable complexes were correlated, indicating their co-regulation, RC assembly factors exhibited modest co-regulation. Within complex I, nuclear DNA-encoded subunits exhibited >2.5-times higher co-regulation than mitochondrial (mt)DNA-encoded subunits. Moreover, mtDNA copy number was unrelated to mtDNA-encoded subunits abundance, suggesting that mtDNA content is not limiting. Alzheimer disease (AD) brains exhibited reduced abundance of complex I RC subunits, an effect largely driven by a 2-4% overall lower mitochondrial protein content. These findings provide foundational knowledge to identify molecular mechanisms contributing to age- and disease-related erosion of mitochondrial function in the human brain.

scholarly journals Mitochondrial respiratory chain super-complex I–III in physiology and pathology

2010 ◽  
Vol 1797 ◽  
pp. 9
Author(s):  
Giorgio Lenaz ◽  
Alessandra Baracca ◽  
Giovanna Barbero ◽  
Christian Bergamini ◽  
Maria Elena Dalmonte ◽  
...  
Keyword(s):  
Respiratory Chain ◽  
Complex I ◽  
Mitochondrial Respiratory Chain ◽  
Mitochondrial Respiratory
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