scholarly journals Comparative evaluation of the effect of cold ceramic and MTA-Angelus on cell viability, attachment and differentiation of dental pulp stem cells and periodontal ligament fibroblasts: an in vitro study

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Sedigheh Khedmat ◽  
Pegah Sarraf ◽  
Ehsan Seyedjafari ◽  
Parisa Sanaei-rad ◽  
Faranak Noori
Keyword(s):  
Cell Viability ◽  
Dental Pulp ◽  
Periodontal Ligament ◽  
In Vitro Study ◽  
Vitro Study ◽  
Control Group ◽  
Periodontal Ligament Fibroblasts ◽  
Pulp Therapy ◽  
Vital Pulp Therapy

Abstract Background Biocompatibility and induction of mineralized tissue formation are the properties expected from a material used in vital pulp therapy and repair of perforations. Cold ceramic (SJM, Iran; CC) is a newly introduced calcium silicate-based cement for above mentioned therapeutic applications. This in-vitro study aimed to compare the effect of CC and White MTA-Angelus (MTA) on cell viability, attachment, odontogenic differentiation, and calcification potential of human dental pulp stem cells (DPSCs) and periodontal ligament fibroblasts (PDLFs). Methods Cell viability of DPSCs and PDLFs was assessed using MTT on days 1, 3, 7, and 14 (n = 9) in contact with freshly mixed and set states of CC and MTA. Field emission scanning electron micrographs (FESEM) were taken to evaluate cell-bioceramic interaction (n = 6). Gene expression levels of osteo/odontogenic markers (Dentin sialophosphoprotein, Dentin matrix protein 1, Collagen type I alpha 1, and Alkaline phosphatase (DSPP, DMP1, COL 1A1, and ALP, respectively) (n = 8) were assessed using qrt-PCR. ALP enzymatic activity was evaluated to assess the mineralization potential. A two-way ANOVA test was applied, and p < 0.05 was considered to be statistically significant. Results The effect of freshly mixed and set MTA and CC on the survival of DPSCs and PDLFs in all study groups was statistically similar and comparable to the positive control group (p > 0.05); the only exception was for the viability of PDLFs in contact with freshly mixed cements on day 1, showing a more significant cytotoxic effect compared to the control and the set state of materials (p < 0.05). PDLFs attached well on CC and MTA. The spread and pseudopodium formation of the cells increased on both samples from day 1 to day 14. Contact of MTA and CC with DPSCs similarly increased expression of all dentinogenesis markers studied on days 7 and 14 compared to the control group (p < 0.001), except for DSPP expression on day 7 (p = 0.46 and p = 0.99 for MTA and CC, respectively). Conclusions Within the limitation of this in-vitro study, cold ceramic and MTA-Angelus showed high biocompatibility and induced increased expression of osteo/dentinogenic markers. Therefore, cold ceramic can be a suitable material for vital pulp therapy and the repair of root perforations.

Coconut milk and probiotic milk as storage media to maintain periodontal ligament cell viability: an in vitro study

2016 ◽  
Vol 33 (3) ◽  
pp. 160-164 ◽  
Author(s):  
Divya Saini ◽  
Prahlad Gadicherla ◽  
Prakash Chandra ◽  
Latha Anandakrishna
Keyword(s):  
Cell Viability ◽  
Periodontal Ligament ◽  
In Vitro Study ◽  
Coconut Milk ◽  
Vitro Study ◽  
Periodontal Ligament Cell ◽  
Storage Media

scholarly journals In Vitro Cytotoxicity Evaluation of Three Root-End Filling Materials in Human Periodontal Ligament Fibroblasts

2016 ◽  
Vol 27 (2) ◽  
pp. 187-191 ◽  
Author(s):  
Hernán Coaguila-Llerena ◽  
Abraham Vaisberg ◽  
Zulema Velásquez-Huamán
Keyword(s):  
Cell Viability ◽  
Periodontal Ligament ◽  
Colorimetric Assay ◽  
Control Group ◽  
Repair Material ◽  
Periodontal Ligament Fibroblasts ◽  
Filling Materials ◽  
Root Repair ◽  
Human Periodontal Ligament Fibroblasts

Abstract The aim of this study was to evaluate in vitro the cytotoxicity on human periodontal ligament fibroblasts of three root-end filling materials: MTA Angelus(r), EndoSequence Root Repair Material Putty(r) and Super EBA(r). A primary culture of human periodontal ligament fibroblasts was previously obtained in order to evaluate the cytotoxicity of the three extracts from the root-end filling materials after 2 and 7 days of setting. Serial dilutions of these extracts (1:1, 1:2, 1:4 and 1:8) were evaluated at 1, 3 and 7 days using the methyl-thiazol-tetrazolium (MTT) colorimetric assay. Cell viability was evaluated as percentage of the negative control group, which represented 100% cell viability. Statistical analyses were done with t-test, ANOVA and Kruskal-Wallis test at a significance level of 5%. It was found that the main difference among root-end filling materials was in the higher dilutions (p<0.05), but there was a similar behavior in lower dilutions (p>0.05). Cell viability of MTA Angelus(r) was superior for 2-day setting (p<0.05), compared with the other two root-end fillings. There were no statistically significant differences between 7-day set MTA Angelus(r) and EndoSequence Root Repair Material Putty(r). Super EBA(r) showed the lowest percentage of cell viability at higher dilutions (p<0.05). Therefore, MTA Angelus(r) and EndoSequence Root Repair Material Putty(r) were less cytotoxic in the highest dilution (1:1) compared with Super EBA(r).

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