scholarly journals Implementation of the agmatine-controlled expression system for inducible gene expression in Lactococcus lactis

2015 ◽  
Vol 14 (1) ◽  
Author(s):  
Daniel M. Linares ◽  
Patricia Alvarez-Sieiro ◽  
Beatriz del Rio ◽  
Victor Ladero ◽  
Begoña Redruello ◽  
...  
Keyword(s):  
Gene Expression ◽  
Lactococcus Lactis ◽  
Expression System ◽  
Inducible Gene Expression ◽  
Inducible Gene

scholarly journals Cumate-Inducible Gene Expression System for Sphingomonads and Other Alphaproteobacteria

2013 ◽  
Vol 79 (21) ◽  
pp. 6795-6802 ◽  
Author(s):  
Andreas Kaczmarczyk ◽  
Julia A. Vorholt ◽  
Anne Francez-Charlot
Keyword(s):  
Gene Expression ◽  
Caulobacter Crescentus ◽  
Paracoccus Denitrificans ◽  
Expression System ◽  
Model Organisms ◽  
Inducible Gene Expression ◽  
Gene Expression System ◽  
Content Type ◽  
Wide Range ◽  
Inducible Gene

ABSTRACTTunable promoters represent a pivotal genetic tool for a wide range of applications. Here we present such a system for sphingomonads, a phylogenetically diverse group of bacteria that have gained much interest for their potential in bioremediation and their use in industry and for which no dedicated inducible gene expression system has been described so far. A strong, constitutive synthetic promoter was first identified through a genetic screen and subsequently combined with the repressor and the operator sites of thePseudomonas putidaF1cym/cmtsystem. The resulting promoter, termed PQ5, responds rapidly to the inducer cumate and shows a maximal induction ratio of 2 to 3 orders of magnitude in the different sphingomonads tested. Moreover, it was also functional in otherAlphaproteobacteria, such as the model organismsCaulobacter crescentus,Paracoccus denitrificans, andMethylobacterium extorquens. In the noninduced state, expression from PQ5is low enough to allow gene depletion analysis, as demonstrated with the essential genephyPofSphingomonassp. strain Fr1. A set of PQ5-based plasmids has been constructed allowing fusions to affinity tags or fluorescent proteins.

Heat-Inducible Gene Expression System by Applying Alternating Magnetic Field to Magnetic Nanoparticles

2013 ◽  
Vol 3 (5) ◽  
pp. 273-279 ◽  
Author(s):  
Masaki Yamaguchi ◽  
Akira Ito ◽  
Akihiko Ono ◽  
Yoshinori Kawabe ◽  
Masamichi Kamihira
Keyword(s):  
Gene Expression ◽  
Magnetic Field ◽  
Magnetic Nanoparticles ◽  
Expression System ◽  
Alternating Magnetic Field ◽  
Inducible Gene Expression ◽  
Gene Expression System ◽  
Inducible Gene

scholarly journals A tunable anthranilate-inducible gene expression system for Pseudomonas species

2020 ◽  
Vol 105 (1) ◽  
pp. 247-258
Author(s):  
Lena Hoffmann ◽  
Michael-Frederick Sugue ◽  
Thomas Brüser
Keyword(s):  
Gene Expression ◽  
Expression System ◽  
Ant System ◽  
Plant Host ◽  
Inducible Gene Expression ◽  
Gene Expression System ◽  
Expression Levels ◽  
Key Points ◽  
Wide Range ◽  
Inducible Gene

Abstract Pseudomonads are among the most common bacteria in soils, limnic ecosystems, and human, animal, or plant host environments, including intensively studied species such as Pseudomonas aeruginosa, P. putida, or P. fluorescens. Various gene expression systems are established for some species, but there is still a need for a simple system that is suitable for a wide range of pseudomonads and that can be used for physiological applications, i.e., with a tuning capacity at lower expression levels. Here, we report the establishment of the anthranilate-dependent PantA promoter for tunable gene expression in pseudomonads. During studies on P. fluorescens, we constructed an anthranilate-inducible AntR/PantA-based expression system, named pUCP20-ANT, and used GFP as reporter to analyze gene expression. This system was compared with the rhamnose-inducible RhaSR/PrhaB-based expression system in an otherwise identical vector background. While the rhamnose-inducible system did not respond to lower inducer concentrations and always reached high levels over time when induced, expression levels of the pUCP20-ANT system could be adjusted to a range of distinct lower or higher levels by variation of anthranilate concentrations in the medium. Importantly, the anthranilate-inducible expression system worked also in strains of P. aeruginosa and P. putida and therefore will be most likely useful for physiological and biotechnological purposes in a wide range of pseudomonads. Key points • We established an anthranilate-inducible gene expression system for pseudomonads. • This system permits tuning of gene expression in a wide range of pseudomonads. • It will be very useful for physiological and biotechnological applications.

scholarly journals Inducible gene expression mediated by a repressor-operator system isolated from Lactococcus lactis bacteriophage r1t

1996 ◽  
Vol 19 (6) ◽  
pp. 1331-1341 ◽  
Author(s):  
Arjen Nauta ◽  
Ouwe Sinderen ◽  
Harma Karsens ◽  
Egbert Smit ◽  
Gerard Venema ◽  
...  
Keyword(s):  
Gene Expression ◽  
Lactococcus Lactis ◽  
Inducible Gene Expression ◽  
Operator System ◽  
Inducible Gene

Development of an inflammation-inducible gene expression system using helper-dependent adenoviral vectors

2010 ◽  
Vol 12 (10) ◽  
pp. 832-839 ◽  
Author(s):  
Tianyao Yang ◽  
Rongqi Duan ◽  
Huibi Cao ◽  
Benjamin H. Lee ◽  
Chun Xia ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression System ◽  
Adenoviral Vectors ◽  
Inducible Gene Expression ◽  
Gene Expression System ◽  
Inducible Gene

Hypoxia-inducible gene expression system using the erythropoietin enhancer and 3′-untranslated region for the VEGF gene therapy

2006 ◽  
Vol 115 (1) ◽  
pp. 113-119 ◽  
Author(s):  
Minhyung Lee ◽  
Donghoon Choi ◽  
Min Ji Choi ◽  
Ji Hoon Jeong ◽  
Won Jong Kim ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Therapy ◽  
Untranslated Region ◽  
Expression System ◽  
Vegf Gene ◽  
Inducible Gene Expression ◽  
Gene Expression System ◽  
Inducible Gene
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