scholarly journals A high throughput assay of lichenase activity with Congo red dye in plants

Plant Methods ◽  
2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Alexander A. Tyurin ◽  
Aleksandra V. Suhorukova ◽  
Igor V. Deineko ◽  
Olga S. Pavlenko ◽  
Viktoriia A. Fridman ◽  
...  
Keyword(s):  
High Throughput ◽  
Congo Red ◽  
Specific Interaction ◽  
High Sensitivity ◽  
Plant Sample ◽  
Regulatory Sequences ◽  
Reporter Protein ◽  
Cost Efficient ◽  
Living Organisms ◽  
Red Dye

Abstract Background Since the beginning of the use of reporter proteins for expression analysis, a variety of approaches have been developed and proposed; both qualitative and quantitative. The lack of simple methods for direct observation of gene expression in living organisms makes it necessary to continue to propose new methods. In this work, we consider a method for the quantitative analysis of the expression of thermostable lichenase from Clostridium thermocellum used as a sensitive reporter protein. Results In this study, we report the design a high throughput fluorometric method for quantification of thermostable lichenase C. thermocellum using Congo red and further experimental verification of its relevance and efficiency in assessment of the functional role of regulatory sequences in the plant cell. Conclusions The specific interaction between the dye Congo red and $$\beta$$ β -d-glucans formed the background for designing a high-throughput fluorometric assay for quantification of C. thermocellum thermostable lichenase as a reporter protein for plants. This assay (i) makes it possible to precisely measure the amount of reporter protein in a plant sample; (ii) has shown a high sensitivity for quantification of thermostable lichenase; (iii) is more time- and cost-efficient as compared with the Somogyi–Nelson assay; and (iv) is to the least degree dependent on the presence of the tested buffer components as compared with the Somogyi–Nelson assay.

A High Throughput Assay of Lichenase Activity with Congo Red Dye in Plants

2021 ◽  
Author(s):  
Alexander Tyurin ◽  
Aleksandra V. Suhorukova ◽  
Igor V. Deineko ◽  
Olga S. Pavlenko ◽  
Viktoriia A. Fridman ◽  
...  
Keyword(s):  
Plant Cell ◽  
High Throughput ◽  
Experimental Verification ◽  
Congo Red ◽  
Functional Role ◽  
Specific Interaction ◽  
Regulatory Sequences ◽  
Reporter Protein ◽  
Verification Methods

Abstract Background : To elucidate the functional role of regulatory sequences and contexts identified and predicted during omics studies in the complex mechanisms of gene expression regulation, experimental verification methods in a plant cell are required. For this, the method of transient expression of reporter genes fused with the tested sequences is widely used. Several reporter systems are available that have shown good performance in the studies including the thermostable lichenase Clostridium thermocellum . However, each reporter system has its own limitations with respect to the quantification of the protein product of a reporter gene and, in particular, for the use of high-throughput approaches. Results: In this study, we report the design a high throughput fluorometric method for quantification of thermostable lichenase C. thermocellum using Congo red and further experimental verification of its relevance and efficiency in assessment of the functional role of regulatory sequences in the plant cell. Conclusion: The specific interaction between the dye Congo red and β -D-glucans formed the background for designing a high-throughput fluorometric assay for quantification of C. thermocellum thermostable lichenase as a reporter protein for plants. This assay (i) makes it possible to precisely measure the amount of reporter protein in a plant sample; (ii) has shown a high sensitivity for quantification of thermostable lichenase; (iii) is more time- and cost-efficient as compared with the Somogyi–Nelson assay; and (iv) is to the least degree dependent on the presence of the tested buffer components as compared with the Somogyi–Nelson assay.

A high throughput colorimetric assay of β-1,3-d-glucans by Congo red dye

2015 ◽  
Vol 109 ◽  
pp. 140-148 ◽  
Author(s):  
Magda C. Semedo ◽  
Amin Karmali ◽  
Luís Fonseca
Keyword(s):  
High Throughput ◽  
Congo Red ◽  
Colorimetric Assay ◽  
Congo Red Dye ◽  
Red Dye

An effective method for determining the retention and distribution of cellulose nanofibrils in paper handsheets by dye labeling

TAPPI Journal ◽  
2018 ◽  
Vol 17 (03) ◽  
pp. 157-164 ◽  
Author(s):  
Shengdan Wang ◽  
Wenhua Gao ◽  
Kefu Chen ◽  
Jinsong Zeng ◽  
Jun Xu ◽  
...  
Keyword(s):  
Correlation Coefficient ◽  
Congo Red ◽  
Kraft Pulp ◽  
Cellulose Nanofibrils ◽  
Standard Curve ◽  
Congo Red Dye ◽  
Mechanical Disintegration ◽  
Softwood Kraft Pulp ◽  
White Water ◽  
Red Dye

Cellulose nanofibrils (CNF) were prepared by cellulase in conjunction with mechanical disintegration from the bleached softwood kraft pulp and labelled by Congo red dye. The labelled CNF were used to investigate the retention and distribution of CNF in paper handsheets. The retention of the labelled CNF was obtained by measuring the absorbance of white water using an ultraviolet-visible spectrophotometer. The results showed that this method for measuring the retention was rapid, feasible, and sensitive, owing to the high correlation coefficient R2 (0.9993) of the standard curve. The labelled CNF showed even distribution in paper handsheets. The colorimetric values of paper handsheets were explored with a residual ink analyzer.

scholarly journals Functionalization of Congo red dye as a light harvester on solar cell

2020 ◽  
Vol 18 (1) ◽  
pp. 287-294
Author(s):  
Harsasi Setyawati ◽  
Handoko Darmokoesoemo ◽  
Irmina Kris Murwani ◽  
Ahmadi Jaya Permana ◽  
Faidur Rochman
Keyword(s):  
Solar Cell ◽  
Congo Red ◽  
Large Scale ◽  
High Efficiency ◽  
Solar Cell Device ◽  
Dye Sensitized ◽  
Congo Red Dye ◽  
Elemental Analyses ◽  
Red Dye ◽  
Solar Cell Technology

AbstractThe demands of ecofriendly technologies to produce a reliable supply of renewable energy on a large scale remains a challenge. A solar cell based on DSSC (Dye-Sensitized Solar Cell) technology is environmentally friendly and holds the promise of a high efficiency in converting sunlight into electricity. This manuscript describes the development of a light harvester system as a main part of a DSSC. Congo red dye has been functionalized with metals (Fe, Co, Ni), forming a series of complexes that serve as a novel light harvester on the solar cell. Metal-congo red complexes have been characterized by UV-VIS and FTIR spectroscopy, and elemental analyses. The performance of metal complexes in capturing photons from sunlight has been investigated in a solar cell device. The incorporation of metals to congo red successfully improved of the congo red efficiency as follows: Fe(II)-congo red, Co(II)-congo red and Ni(II)-congo red had efficiencies of 8.17%, 6.13% and 2.65%, respectively. This research also discusses the effect of metal ions on the ability of congo red to capture energy from sunlight.

scholarly journals Spent Coffee Ground as Low-Cost Adsorbent for Congo Red Dye Removal from Aqueous Solution

2021 ◽  
Vol 765 (1) ◽  
pp. 012089
Author(s):  
R Taufik ◽  
M Mohamad ◽  
R Wannahari ◽  
N F Shoparwe ◽  
WHW Osman ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Congo Red ◽  
Dye Removal ◽  
Low Cost ◽  
Congo Red Dye ◽  
Spent Coffee Ground ◽  
Coffee Ground ◽  
Red Dye

scholarly journals From Multiplex Serology to Serolomics—A Novel Approach to the Antibody Response against the SARS-CoV-2 Proteome

Viruses ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 749
Author(s):  
Julia Butt ◽  
Rajagopal Murugan ◽  
Theresa Hippchen ◽  
Sylvia Olberg ◽  
Monique van Straaten ◽  
...  
Keyword(s):  
Antibody Response ◽  
High Throughput ◽  
Large Population ◽  
High Sensitivity ◽  
Population Based ◽  
Antibody Responses ◽  
Hek293 Cells ◽  
Novel Approach ◽  
Assay Performance ◽  
Multiplex Serology

The emerging SARS-CoV-2 pandemic entails an urgent need for specific and sensitive high-throughput serological assays to assess SARS-CoV-2 epidemiology. We, therefore, aimed at developing a fluorescent-bead based SARS-CoV-2 multiplex serology assay for detection of antibody responses to the SARS-CoV-2 proteome. Proteins of the SARS-CoV-2 proteome and protein N of SARS-CoV-1 and common cold Coronaviruses (ccCoVs) were recombinantly expressed in E. coli or HEK293 cells. Assay performance was assessed in a COVID-19 case cohort (n = 48 hospitalized patients from Heidelberg) as well as n = 85 age- and sex-matched pre-pandemic controls from the ESTHER study. Assay validation included comparison with home-made immunofluorescence and commercial enzyme-linked immunosorbent (ELISA) assays. A sensitivity of 100% (95% CI: 86–100%) was achieved in COVID-19 patients 14 days post symptom onset with dual sero-positivity to SARS-CoV-2 N and the receptor-binding domain of the spike protein. The specificity obtained with this algorithm was 100% (95% CI: 96–100%). Antibody responses to ccCoVs N were abundantly high and did not correlate with those to SARS-CoV-2 N. Inclusion of additional SARS-CoV-2 proteins as well as separate assessment of immunoglobulin (Ig) classes M, A, and G allowed for explorative analyses regarding disease progression and course of antibody response. This newly developed SARS-CoV-2 multiplex serology assay achieved high sensitivity and specificity to determine SARS-CoV-2 sero-positivity. Its high throughput ability allows epidemiologic SARS-CoV-2 research in large population-based studies. Inclusion of additional pathogens into the panel as well as separate assessment of Ig isotypes will furthermore allow addressing research questions beyond SARS-CoV-2 sero-prevalence.

Sodium alginate and polypyrrole composites with algal dead biomass for the adsorption of Congo Red dye: Kinetics, thermodynamics and desorption studies

2021 ◽  
pp. 101183
Author(s):  
Munazza Maqbool ◽  
Sana Sadaf ◽  
Haq N. Bhatti ◽  
Sehrish Rehmat ◽  
Abida Kausar ◽  
...  
Keyword(s):  
Sodium Alginate ◽  
Congo Red ◽  
Dead Biomass ◽  
Congo Red Dye ◽  
Red Dye
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