scholarly journals Effect of different storage solutions on oxidative stress in human saphenous vein grafts

2022 ◽  
Vol 17 (1) ◽  
Author(s):  
Ilker Tekin ◽  
Meltem Demir ◽  
Sebahat Özdem

Abstract Background Ischemia–reperfusion injury of saphenous vein grafts (SVG) during coronary artery bypass grafting surgery negatively impacts endothelial integrity and functionality and is associated with vein graft failure. The aim of this study was to evaluate the level of oxidative stress in human SVG segments following ischemic storage in three intraoperative graft storage solutions: saline (S), autologous heparinized blood (HB) and DuraGraft (DG). Methods 3 mm tissue rings derived from surplus SVG segments from 50 patients were stored at room temperature for 30 min in DG, S or HB. Total oxidative status (TOS) and total antioxidant status (TAS) levels were determined from which the oxidative stress index (OSI: TOS/TAS ratio) was calculated. A p-value < 0.017 was considered significant implementing a Bonferroni correction. Results TOS values were significantly lower for DG stored samples in comparison to both S and HB; there was no difference between S and HB (DG: 32.6 ± 1.8, S: 39.6 ± 2.8 and HB: 40.6 ± 2.4 µmol H2O2 eqv.; DG vs. S and DG vs. HB p < 0.0001, S vs. HB p = 0.047). TAS was higher for both DG and HB in comparison to S (DG: 8.9 ± 0.9, S: 6.9 ± 1.0 and HB: 8.6 ± 0.9 mmol Trolox eqv.; DG vs S p < 0.0001, DG vs. HB p = 0.263, S vs. HB p < 0.0001). OSI differed between all groups with the lowest value for DG (DG: 3.7 ± 0.2, S: 5.8 ± 0.4 and HB: 4.7 ± 0.2 µmol H2O2 eqv./mmol Trolox eqv.; all p < 0.0001). Conclusions Saphenous veins grafts stored in DuraGraft had a lower oxidative level, higher antioxidant level and a lower oxidative stress index in comparison to saphenous vein grafts stored in saline or heparinized blood. ClinicalTrials.gov Identifier NCT02922088.

2021 ◽  
Author(s):  
İLKER TEKİN ◽  
MELTEM DEMİR ◽  
SEBAHAT ÖZDEM

Abstract Background: Ischemic injury of saphenous vein grafts (SVG) during coronary artery bypass grafting surgery negatively impacts endothelial integrity and functionality and is associated with vein graft disease and failure. The aim of this study was to evaluate the level of oxidative stress in human SVG segments following ischemic storage in three intraoperative graft storage solutions: physiological saline (PS), autologous heparinized blood (HB) and DuraGraft.Methods: 3 mm tissue rings derived from surplus SVG segments from 50 patients were stored at room temperature for 30 min in DuraGraft, PS and HB. Total oxidative status (TOS) and total antioxidant status (TAS) levels were determined and the oxidative stress index (OSI: TOS/TAS ratio) calculated. A p-value <0.017 was considered significant implementing a Bonferroni correction.Results: TOS values were significantly lower for DuraGraft stored samples in comparison to both PS and HB; there was no difference between PS and HB (DuraGraft: 32.6±1.8, PS: 39.6±2.8 and HB: 40.6±2.4 µmol H2O2 eqv.; DuraGraft vs. PS and DuraGraft vs. HB p<0.0001, PS vs. HB p=0.047). TAS was higher for both DuraGraft and HB in comparison to PS (DuraGraft: 8.9±0.9, PS: 6.9±1.0 and HB: 8.6±0.9 mmol Trolox eqv.; DuraGraft vs PS p<0.0001, DuraGraft vs. HB p=0.263, PS vs. HB p<0.0001). OSI differed between all groups with the lowest value for DuraGraft (DuraGraft: 3.7±0.2, PS: 5.8±0.4 and HB: 4.7±0.2 µmol H2O2 eqv./mmol Trolox eqv.; all p<0.0001).Conclusions: saphenous veins grafts stored in DuraGraft had a lower oxidative stress level, higher antioxidant level and lower oxidative stress index in comparison to saphenous vein grafts stored in physiological saline or heparinized blood.ClinicalTrials.gov Identifier: NCT02922088


2020 ◽  
Vol 50 (6) ◽  
pp. 1513-1522
Author(s):  
Şenol KALYONCU ◽  
Bülent YILMAZ ◽  
Mustafa DEMİR ◽  
Meltem TUNCER ◽  
Zehra BOZDAĞ ◽  
...  

Background/aim: To evaluate the protective effect of melatonin on ovarian ischemia reperfusion injury in a rat model. Materials and methods: Forty-eight rats were separated equally into 6 groups. Group 1: sham; Group 2: surgical control with 3-h bilateral ovarian torsion and detorsion; Group 3: intraperitoneal 5% ethanol (1 mL) just after detorsion (as melatonin was dissolved in ethanol); Group 4: 10 mg/kg intraperitoneal melatonin 30 min before 3-h torsion; Group 5:10 mg/kg intraperitoneal melatonin just after detorsion; Group 6:10 mg/kg intraperitoneal melatonin 30 min before torsion and just after detorsion. Both ovaries and blood samples were obtained 7 days after detorsion for histopathological and biochemical analysis.Results: In Group 1, serum levels of total oxidant status (TOS) (μmol H2O2 equivalent/g wet tissue)were significantly lower than in Group2 (P = 0.0023), while tissue TOS levels were lower than in Group 3 (P = 0.0030). Similarly, serum and tissue levels of peroxynitrite in Group 6were significantly lower than those ofGroup 2 (P = 0.0023 and P = 0.040, respectively). Moreover, serum oxidative stress index (OSI) (arbitrary unit) levels were significantly increased in Group 2 when compared to groups 1 and 6 (P = 0.0023 and P= 0.0016, respectively) and in Group 3 with respect to groups 1, 4, 5, and 6 (P = 0.0023, P = 0.0026, P = 0.0008, and P = 0.0011, respectively). Furthermore, there was a significant decrease in histopathological scores including follicular degeneration, vascular congestion, hemorrhage, and inflammation in the melatonin and sham groups in comparison with control groups. Additionally, primordial follicle count was significantly higher in Group 6 than in Group 2 (P = 0.0002).Conclusion: Melatonin attenuates ischemia reperfusion damage in a rat torsion/detorsion model by improving histopathological and biochemical findings including OSI and peroxynitrite.


2017 ◽  
Vol 104 (2) ◽  
pp. 560-567 ◽  
Author(s):  
Hiroshi Kubota ◽  
Hidehito Endo ◽  
Hikaru Ishii ◽  
Hiroshi Tsuchiya ◽  
Yu Takahashi ◽  
...  

2020 ◽  
Vol 315 ◽  
pp. e249
Author(s):  
T. Kalkan ◽  
C. Bintepe ◽  
I. Yurekli ◽  
N. Ersoy ◽  
H.A. Bagriyanik ◽  
...  

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