Effect of different storage solutions on oxidative stress in human saphenous vein grafts

Background Ischemia–reperfusion injury of saphenous vein grafts (SVG) during coronary artery bypass grafting surgery negatively impacts endothelial integrity and functionality and is associated with vein graft failure. The aim of this study was to evaluate the level of oxidative stress in human SVG segments following ischemic storage in three intraoperative graft storage solutions: saline (S), autologous heparinized blood (HB) and DuraGraft (DG). Methods 3 mm tissue rings derived from surplus SVG segments from 50 patients were stored at room temperature for 30 min in DG, S or HB. Total oxidative status (TOS) and total antioxidant status (TAS) levels were determined from which the oxidative stress index (OSI: TOS/TAS ratio) was calculated. A p-value < 0.017 was considered significant implementing a Bonferroni correction. Results TOS values were significantly lower for DG stored samples in comparison to both S and HB; there was no difference between S and HB (DG: 32.6 ± 1.8, S: 39.6 ± 2.8 and HB: 40.6 ± 2.4 µmol H2O2 eqv.; DG vs. S and DG vs. HB p < 0.0001, S vs. HB p = 0.047). TAS was higher for both DG and HB in comparison to S (DG: 8.9 ± 0.9, S: 6.9 ± 1.0 and HB: 8.6 ± 0.9 mmol Trolox eqv.; DG vs S p < 0.0001, DG vs. HB p = 0.263, S vs. HB p < 0.0001). OSI differed between all groups with the lowest value for DG (DG: 3.7 ± 0.2, S: 5.8 ± 0.4 and HB: 4.7 ± 0.2 µmol H2O2 eqv./mmol Trolox eqv.; all p < 0.0001). Conclusions Saphenous veins grafts stored in DuraGraft had a lower oxidative level, higher antioxidant level and a lower oxidative stress index in comparison to saphenous vein grafts stored in saline or heparinized blood. ClinicalTrials.gov Identifier NCT02922088.

Application of SP AW model parameters - optimum sowing date and water stress to explain wheat yield variability

The Soil-Plant-Atmosphere- Water (SPA W) model has been calibrated and validated using field experiment data from 1991-92 to 1993-94 for wheat crop at Varanasi district. Long-term (1973-74 to 1995-96) daily weather data were combined with general observation of wheat growth and soils to provide daily water budgets for 23 years. The model was calibrated with one year detailed crop growth characteristics and soil water observations and validated with another year soil water observations. The daily-integrated water stress index (WSI) values at the end of crop season correlated quite well with observed grain yield in this region. The water budget analysis shows a distinct optimum sowing period from 5th to 25th November and an optimum sowing date on 15th November with minimal water stress index. These results demonstrate the potential of SPA W model for planning irrigation scheduling and water management for wheat crop in this region.

CROP WATER STRESS INDEX FOR PREDICTING YIELD LOSS IN COMMON BEAN

The crop water stress index (CWSI), an index derived from canopy temperature, has been widely studied as a physiological indicator of plant water status to optimize irrigation in common beans. However, it is not clear how this index could contribute to yield prediction as a decision support tool in irrigation management. This paper aimed to use the CWSI for predicting yield loss in common bean (Phaseolus vulgaris L.) subjected to water stress under drip irrigation. A rain shelter experiment was conducted using a completely randomized design with five replications. The indeterminate growth cultivar TAA Dama was subjected to three irrigation treatments: 100% of the field capacity (FC), 75 and 50% FC from 20 days after sowing (DAS) until the end of the crop cycle. Grain yield was reduced by 42% under 50% FC treatment. Furthermore, stomatal conductance was reduced under this treatment, whereas the CWSI and canopy temperature increased as irrigation levels decreased. The relationship between grain yield and CWSI (R2=0.76, RSME=2.35g) suggests that canopy temperature data could be used to forecast grain yield losses. In conclusion, farmers can have a low-cost, effective technique for making water management decisions in common bean.

Kaolin Film Increases Gas Exchange Parameters of Coffee Seedlings During Transference From Nursery to Full Sunlight

Increases in water use efficiency (WUE) and the reduction of negative impacts of high temperatures associated with high solar radiation are being achieved with the application of fine particle film of calcined and purified kaolin (KF) on the leaves and fruits of various plant species. KF was applied on young Coffea arabica and Coffea canephora plants before their transition from nursery to full sunlight during autumn and summer. The effects of KF were evaluated through the responses of leaf temperature (Tleaf), net CO2 assimilation rate (A), stomatal conductance (gs), transpiration (E), WUE, crop water stress index (CWSI), index of relative stomatal conductance (Ig), initial fluorescence (F0), and photosynthetic index (PI) in the first 2–3 weeks after the plant transitions to the full sun. All measurements were performed at midday. In Coffea plants, KF decreased the Tleaf up to 6.7°C/5.6°C and reduced the CWSI. The plants that were not protected with KF showed lower A, gs, E, and Ig than those protected with KF. C. canephora plants protected with KF achieved higher WUE compared with those not protected by 11.23% in autumn and 95.58% in summer. In both Coffea sp., KF application reduced F0, indicating reduced physical dissociation of the PSII reaction centers from the light-harvesting system, which was supported with increased PI. The use of KF can be recommended as a management strategy in the transition of Coffea seedlings from the nursery shade to the full sunlight, to protect leaves against the excessive solar radiation and high temperatures, especially in C. canephora during the summer.

Maternal Depression and Early Parenting: A Comparison Between Culturally and Linguistically Diverse and Australian born Mothers

Objective: To examine the risk of perinatal depression, parenting stress and infant sleep practices in Australian culturally and linguistically diverse (CaLD) women. Method: Within the Mercy Pregnancy and Emotional Wellbeing Study, we examined 487 pregnant women of whom 52 were CaLD and 435 non-CaLD. Depression was measured using the Structured Clinical Interview for DSM-IV and the Edinburgh Postnatal Depression Scale. In addition, Parenting Stress Index and infant sleep measures were collected. Results: Fewer CaLD women had a depression diagnosis but there were no differences between CaLD and non-CaLD women for perinatal mental health symptoms. More mothers in the CaLD group were bed sharing with their infant during the night at six months; however, bedsharing was only associated with higher parenting stress for non-CaLD mothers. Conclusions: Findings suggest both differences in infant sleep parenting practices and in parenting stress but not general emotional wellbeing. Future research is required to replicate these findings.

Metabolic Stress Index Including Mitochondrial Biomarker for Noninvasive Diagnosis of Hepatic Steatosis

Background Mitochondrial dysfunction with oxidative stress contributes to nonalcoholic fatty liver disease (NAFLD) progression. We investigated the steatosis predictive efficacy of a novel non-invasive diagnostic panel using metabolic stress biomarkers. Methods Altogether, 343 subjects who underwent magnetic resonance imaging-based liver examinations from a population-based general cohort, and 41 patients enrolled in a biopsy-evaluated NAFLD cohort, participated in the development and validation groups, respectively. Serologic stress biomarkers were quantitated by enzyme-linked immunosorbent assay. Results Multivariate regression showed that waist-to-hip ratio, fibroblast growth factor (FGF) 21, FGF19, adiponectin-to-leptin ratio, insulin, albumin, triglyceride, total-cholesterol, and alanine-aminotransferase were independent predictors of steatosis (rank-ordered by Wald). The area under receiver-operator characteristics curve [AUROC (95%CI)] of the metabolic stress index for steatosis (MSI-S) was 0.886 (0.85‒0.92) and 0.825 (0.69‒0.96) in development and validation groups, respectively. MSI-S had higher diagnostic accuracy (78.1%‒81.1%) than other steatosis indices. MSI-S notably differentiated steatosis severities, while other indices showed less discrimination. Conclusion MSI-S, as a novel non-invasive index, based on mitochondrial stress biomarker FGF21 effectively predicted steatosis. Furthermore, MSI-S may increase the population that could be excluded from further evaluation, reducing unnecessary invasive investigations more effectively than other indices.

Behaviour of relative evapotranspiration with agrometeorological stress indices in wheat

Complex behaviour of stress indices with relative evapotranspiration was observed in early and late sown wheat, however, under normal sown conditions it was linearly decreasing. Predawn leaf water potential and transpiration rate proved to be a stable stress index parameter for characterizing the internal moisture status in the plant as compared to the canopy temperature and stomatal resistance under stress conditions in wheat. Since it is easy to quantify canopy/leaf temperature and within seasonal variations it is widely used for scheduling irrigation and quantigying moisture stress effects on growth and development in wheat.

Occupational Stress: Associated Factors, Related Symptoms, and Coping Strategies Among Secondary School-heads

This study examined occupational stress, its related symptoms, associated factors, and coping strategies among heads of secondary schools in Kohat Division, Pakistan. A sample of 101 secondary school-heads was taken using a multistage sampling technique. Occupational Stress Index (Shrivastava & Singh, 1981) followed by four open-ended questions was used to investigate the study variables. Findings revealed that heads of secondary schools were occupationally stressed in their workplace. The most associated factors causing occupational stress were poor compensation, work overload, lack of effective advancement and promotion policy, poor implementation of education policy, lack of basic facilities, political interference, and under-participation. Several symptoms such as headaches, alterations in blood pressure, and digestive problems, were found to be associated with an increased risk of occupational stress. Furthermore, various related perceived physiological, psychological, and behavioral consequences were found to be caused by stress at work. Findings suggest that a comprehensive strategy promoted by the education department should be recommended for reducing stress among secondary school-heads and improving their wellbeing and other health-related conditions at the workplace.

Analysis of Near-Surface Temperature Lapse Rates in Mountain Ecosystems of Northern Mexico Using Landsat-8 Satellite Images and ECOSTRESS

Mountain ecosystems provide environmental goods, which can be threatened by climate change. Near-Surface Temperature Lapse Rate (NSTLR) is an essential factor used for thermal and hydrological analysis in mountain ecosystems. The aims of the present study were to estimate NSTLR and to identify its relationship with aspect, Local solar zenith angle (LSZA) and Evaporative Stress Index (ESI) for two seasons of the year in a mountain ecosystem at the North of Mexico. Normalized Land Surface Temperature (NLST) was estimated using environmental and topographical variables. LSZA was calculated from slope to consider the effect of solar position. NSTLR was estimated through simple linear models. Observed NSTLR was 9.4 °C km−1 for the winter and 14.3 °C km−1 for the summer. Our results showed variation in NSTLR by season. In addition, aspect, LSZA and ESI also influenced NSTLR regulation. In addition, Northwest and West aspects exhibited the highest NSTLR. LSZA angles closest to 90° were related with a decrease in NSTLR for both seasons. Finally, ESI values associated with less evaporative stress were related to lower NSTLR. These results suggest potential of Landsat-8 LST and ECOSTRESS ESI to capture interactions of temperature, topography, and water stress in complex ecosystems.

Digital Health Services through Patient Empowerment: Classification, Current State and Preliminary Impact Assessment by Health Pod Systems

Health pods are new systems such as small spaces equipped with medical devices where users can measure several biomedical parameters related to their health status and receive other medical services. Their impact on health over a life course could be relevant in defining healthy aging strategies and/or management of chronic diseases and the early detection of possible symptoms related to some common pathologies. The generated data have not only a personal value but even at a community/society level. Health pods also support educational and empowerment actions to enable the 5P medicine approach, and specifically prevention, health promotion, and public health policymaking. This paper aims at defining their taxonomy, conducting a market and typologies survey, and discussing their potential impact in preventive medicine, presenting data of a pilot test carried out placing two health pods in a superstore environment to validate the demand and the participation of people in a prevention campaign. A 57-day period was observed at two sites: the number of free accesses and administered tests was impressive for size and completeness. The test revealed a good picture of the general health status of the population, with satisfying AGE values in the cardiovascular check and stress index through an HRV analysis. The body composition test revealed a small number of overweight subjects, more in males than in females. This pilot confirmed the huge demand for personalized services for improving well-being, health status, and quality of life and the relevance of these solutions for their individual and societal impact in preventive medicine.