scholarly journals Validation and evaluation of a common biomarker in human cancers sera protein detected by a monoclonal antibody UNIVmAb

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
D. Manjunath ◽  
Sunil B. Kumaraswamy ◽  
Shashidhar Aladhi Venkatakrishniah ◽  
Hitesh Nidumanda Appaiah ◽  
Anil Thomas ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Western Blot ◽  
Anion Exchange ◽  
Binding Protein ◽  
Anion Exchange Column ◽  
Over Expression ◽  
Exchange Column ◽  
Human Cancers ◽  
Exclusion Chromatography ◽  
Hyaluronan Binding

Abstract Objective Management and diagnosis of multiple human cancers remains a challenge and search for a common biomarker is still debatable. In this manuscript we have evaluated the use of monoclonal antibody UNIVmAb, to detect the protein (H11) as a common biomarker for all cancers irrespective of the grade and origin. We have shown by both ELISA and Western Blot that the H11 protein, is a unique hyaluronan binding protein that has not been detected earlier. H11 protein was fractionated in an anion exchange column followed by cibacron blue gel exclusion chromatography. Hyaluronan binding H11 protein reacted with Monoclonal antibody UNIVmAb and b-HA inspite of b-Hyaluronan (biotinylated Hyaluronan) interaction and HA-Oligo (Hyaluronan oligosaccharides) competition from various grades of Human cancers sera. Results ELISA, Western blot and b-Hyaluronan interactions clearly showed an over-expression of UNIVmAb reacted H11 protein in all fifty cancer’s sera when compared with seventy normal sera. UNIVmAb reactive H11 protein can be used as a common biomarker. We believe, UNIVmAb detected H11 protein, is a unique hyaluronan binding protein, that can be used as a common biomarker for all cancers.

scholarly journals Validation and evaluation of a common biomarker in human cancers sera protein detected by a monoclonal antibody UNIVMab

2019 ◽  
Author(s):  
Manjunath Dandi ◽  
Sunil B Kumaraswamy ◽  
Shashidhara A V ◽  
Hitesh N Appaiah ◽  
Anil Thomas ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Hyaluronic Acid ◽  
Western Blot ◽  
Anion Exchange ◽  
Binding Protein ◽  
Over Expression ◽  
Acid Binding Protein ◽  
Human Cancers ◽  
Hyaluronan Binding ◽  
Hyaluronic Acid Binding

Abstract Abstract Objective: Management and diagnosis of multiple human cancers remain a challenge and search for a common biomarker are still debatable. We described a method and evaluated the use of monoclonal antibody UNIVMab, to detect the protein (H11) as a common biomarker for all cancer irrespective of grade and origin . H11 protein identified as a unique Hyaluronan binding protein not detected earlier. We applied this test both with ELISA, Western blot, fractionated in anion exchange, cibacron gel exclusion, b-Hyaluronan interaction and HA-Oligo competition from various grades of Human cancers sera and processed for the detection of hyaluronan binding protein H11, reacted with Monoclonal antibody UNIVMab and with b-HA. Results: Studies from ELISA, Western blot and b-Hyaluronan interactions showed a definitive positive over-expression of UNIVMab reacted H11 antigen in all fortyfive cancer sera compared to normal sera and can be used as a common biomarker. We believe, UNIVMab detected H11 antigen, a unique hyaluronan binding protein, is a common biomarker for all cancer sera. Keywords: ELISA, Western blot, Hyaluronic acid binding protein, H11 (sera antigen), UNIVMab. Common Biomarker, Cancers Sera

scholarly journals Validation and evaluation of common biomarker in human cancers sera protein detected by a monoclonal antibody UNIVMab

2019 ◽  
Author(s):  
Manjunath Dandi ◽  
Sunil B Kumaraswamy ◽  
Shashidhara A V ◽  
Hitesh N Appaiah ◽  
Anil Thomas ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Hyaluronic Acid ◽  
Western Blot ◽  
Anion Exchange ◽  
Binding Protein ◽  
Over Expression ◽  
Acid Binding Protein ◽  
Human Cancers ◽  
Hyaluronan Binding ◽  
Hyaluronic Acid Binding

Abstract Objective: Management and diagnosis of multiple human cancers remain a challenge and search for a common biomarker are still debatable. We described a method and evaluated the use of monoclonal antibody UNIVMab, to detect the protein (H11) as a common biomarker for all cancer irrespective of grade and origin . H11 protein identified as a unique Hyaluronan binding protein not detected earlier. We applied this test both with ELISA, Western blot, fractionated in anion exchange, cibacron gel exclusion, b-Hyaluronan interaction and HA-Oligo competition from various grades of Human cancers sera and processed for the detection of hyaluronan binding protein H11, reacted with Monoclonal antibody UNIVMab and with b-HA. Results: Studies from ELISA, Western blot and b-Hyaluronan interactions showed a definitive positive over-expression of UNIVMab reacted H11 antigen in all fortyfive cancer sera compared to normal sera and can be used as a common biomarker. We believe, UNIVMab detected H11 antigen, a unique hyaluronan binding protein, is a common biomarker for all cancer sera. Keywords: ELISA, Western blot, Hyaluronic acid binding protein, H11 (sera antigen), UNIVMab. Common Biomarker, Cancers Sera

scholarly journals Validation and evaluation of a common biomarker in human cancers sera protein detected by a monoclonal antibody UNIVMab

2019 ◽  
Author(s):  
Manjunath Dandi ◽  
Sunil B Kumaraswamy ◽  
Shashidhara A V ◽  
Hitesh N Appaiah ◽  
Anil Thomas ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Hyaluronic Acid ◽  
Western Blot ◽  
Anion Exchange ◽  
Binding Protein ◽  
Over Expression ◽  
Acid Binding Protein ◽  
Human Cancers ◽  
Hyaluronan Binding ◽  
Hyaluronic Acid Binding

Abstract Abstract Objective: Management and diagnosis of multiple human cancers remain a challenge and search for a common biomarker are still debatable. We described a method and evaluated the use of monoclonal antibody UNIVMab, to detect the protein (H11) as a common biomarker for all cancer irrespective of grade and origin . H11 protein identified as a unique Hyaluronan binding protein not detected earlier. We applied this test both with ELISA, Western blot, fractionated in anion exchange, cibacron gel exclusion, b-Hyaluronan interaction and HA-Oligo competition from various grades of Human cancers sera and processed for the detection of hyaluronan binding protein H11, reacted with Monoclonal antibody UNIVMab and with b-HA. Results: Studies from ELISA, Western blot and b-Hyaluronan interactions showed a definitive positive over-expression of UNIVMab reacted H11 antigen in all fortyfive cancer sera compared to normal sera and can be used as a common biomarker. We believe, UNIVMab detected H11 antigen, a unique hyaluronan binding protein, is a common biomarker for all cancer sera. Keywords: ELISA, Western blot, Hyaluronic acid binding protein, H11 (sera antigen), UNIVMab. Common Biomarker, Cancers Sera

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