scholarly journals Development, characterization, and cross-amplification of polymorphic microsatellite markers for North American Trachymyrmex and Mycetomoellerius ants

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Alix E. Matthews ◽  
Chase Rowan ◽  
Colby Stone ◽  
Katrin Kellner ◽  
Jon N. Seal
Keyword(s):  
Microsatellite Markers ◽  
North American ◽  
Cross Amplification ◽  
Polymorphic Microsatellite

scholarly journals Development, characterization, and cross-amplification of polymorphic microsatellite markers for North American Trachymyrmex and Mycetomoellerius ants

2020 ◽  
Author(s):  
Alix E. Matthews ◽  
Chase Rowan ◽  
Colby Stone ◽  
Katrin Kellner ◽  
Jon N. Seal
Keyword(s):  
Microsatellite Markers ◽  
North American ◽  
Polymorphic Markers ◽  
Model Group ◽  
Cross Amplification ◽  
Attine Ants ◽  
Amplification Success ◽  
Genetic Patterns ◽  
Polymorphic Microsatellite ◽  
Generation Sequencing

Abstract Objective The objective of this study is to develop and identify polymorphic microsatellite markers for fungus-gardening (attine) ants in the genus Trachymyrmex sensu lato . These ants are important ecosystem engineers and have been a model group for understanding complex symbiotic systems, but very little is understood about the intraspecific genetic patterns across most North American attine species. These microsatellite markers will help to better study intraspecific population genetic structure, gene flow, mating habits, and phylogeographic patterns in these species and potentially other congeners. Results Using next-generation sequencing techniques, we identified 17 and 12 polymorphic microsatellite markers from T. septentrionalis and Mycetomoellerius (formerly Trachymyrmex ) turrifex , respectively, and assessed the genetic diversity of each marker. We also analyzed the cross-amplification success of the T. septentrionalis markers in two other closely related Trachymyrmex species, and identified 10 and 12 polymorphic markers for T. arizonensis and T. pomonae , respectively.

scholarly journals Development, characterization, and cross-amplification of polymorphic microsatellite markers for North American Trachymyrmex and Mycetomoellerius ants

2020 ◽  
Author(s):  
Alix E. Matthews ◽  
Chase Rowan ◽  
Colby Stone ◽  
Katrin Kellner ◽  
Jon N. Seal
Keyword(s):  
Microsatellite Markers ◽  
North American ◽  
Polymorphic Markers ◽  
Model Group ◽  
Cross Amplification ◽  
Attine Ants ◽  
Amplification Success ◽  
Genetic Patterns ◽  
Polymorphic Microsatellite ◽  
Generation Sequencing

Abstract Objective The objective of this study is to develop and identify polymorphic microsatellite markers for fungus-gardening (attine) ants in the genus Trachymyrmex sensu lato . These ants are important ecosystem engineers and have been a model group for understanding complex symbiotic systems, but very little is understood about the intraspecific genetic patterns across most North American attine species. These microsatellite markers will help to better study intraspecific population genetic structure, gene flow, mating habits, and phylogeographic patterns in these species and potentially other congeners. Results Using next-generation sequencing techniques, we identified 17 and 12 polymorphic microsatellite loci from T. septentrionalis and Mycetomoellerius (formerly Trachymyrmex ) turrifex , respectively, and assessed the genetic diversity of each locus. We also analyzed the cross-amplification success of the T. septentrionalis markers in two other closely related Trachymyrmex species, and identified 10 and 12 polymorphic markers for T. arizonensis and T. pomonae , respectively.

scholarly journals Cross-amplification and Characterization of Polymorphic Microsatellite Markers From Acacia (Senegalia) mellifera and Acacia brevispica to Acacia senegal (L.) Willd.

2010 ◽  
Vol 59 (1-6) ◽  
pp. 285-288 ◽  
Author(s):  
Stephen F. Omondi ◽  
O. G. Dangasuk ◽  
D. W. Odee ◽  
S. Cavers ◽  
D. P. Khasa
Keyword(s):  
Microsatellite Markers ◽  
Allelic Diversity ◽  
Breeding Systems ◽  
Acacia Senegal ◽  
Cross Amplification ◽  
Expected Heterozygosity ◽  
Potential Source ◽  
Polymorphic Microsatellite ◽  
Acacia Mellifera

Abstract Seven polymorphic microsatellite markers isolated from Acacia brevispica and Acacia mellifera were successfully cross-amplified in Acacia senegal. The loci were surveyed for polymorphism using 30 samples. Allelic diversity ranged from 4 (Ame02, Ab06 and Ab18) to 13 (Ab26) per locus. The expected heterozygosity (HE) ranged from 0.543 (Ame02) to 0.868 (Ab26) while observed heterozygosity (HO) ranged from 0.516 (Ame05) to 0.800 (Ame03). Cross-amplification of these loci represents a potential source of co-dominant markers and will be useful in the study of genetic diversity, structure, gene flow and breeding systems of this important Acacia species.

scholarly journals Development and characterization of thirteen novel microsatellite markers for use in Greenland sharks (Somniosus microcephalus), with cross-amplification in Pacific sleeper sharks (Somniosus pacificus)

2020 ◽  
Author(s):  
Meaghan A Swintek ◽  
Ryan P Walter
Keyword(s):  
Genetic Variation ◽  
Microsatellite Markers ◽  
Shark Species ◽  
Bonferroni Correction ◽  
Cross Amplification ◽  
Population Analyses ◽  
Polymorphic Microsatellite ◽  
Somniosus Microcephalus ◽  
Successful Amplification

Abstract Objective The objectives of this research are to isolate, develop and characterize polymorphic microsatellite markers for use in Greenland sharks ( Somniosus microcephalus ). Despite utility in population analyses, microsatellite markers have not been previously developed for this species. Development of these markers, and successful amplification in closely related Pacific sleeper sharks ( S. pacificus ), will facilitate research in the genetic variation of contemporary and future populations of sleeper shark species. Results Thirteen microsatellite loci were successfully amplified and yielded multi-locus genotypes for 32 S. microcephalus individuals from Grise Fjord (n = 16) and Svalbard (n = 20). Each locus yielded between 2 to 9 alleles and observed heterozygosity ranged from 0.11 to 0.70 when estimated across both sites. One locus and three loci deviated from HWE following Bonferroni correction, for individuals sampled from Grise Fjord and Svalbard, respectively. Cross-amplification was successful at every locus for five of the ten S. pacificus individuals.

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