Development, characterization, and cross-amplification of polymorphic microsatellite markers for North American Trachymyrmex and Mycetomoellerius ants

Abstract Objective The objective of this study is to develop and identify polymorphic microsatellite markers for fungus-gardening (attine) ants in the genus Trachymyrmex sensu lato . These ants are important ecosystem engineers and have been a model group for understanding complex symbiotic systems, but very little is understood about the intraspecific genetic patterns across most North American attine species. These microsatellite markers will help to better study intraspecific population genetic structure, gene flow, mating habits, and phylogeographic patterns in these species and potentially other congeners. Results Using next-generation sequencing techniques, we identified 17 and 12 polymorphic microsatellite markers from T. septentrionalis and Mycetomoellerius (formerly Trachymyrmex ) turrifex , respectively, and assessed the genetic diversity of each marker. We also analyzed the cross-amplification success of the T. septentrionalis markers in two other closely related Trachymyrmex species, and identified 10 and 12 polymorphic markers for T. arizonensis and T. pomonae , respectively.

Download Full-text

Development, characterization, and cross-amplification of polymorphic microsatellite markers for North American Trachymyrmex and Mycetomoellerius ants

10.21203/rs.2.21905/v1 ◽

2020 ◽

Author(s):

Alix E. Matthews ◽

Chase Rowan ◽

Colby Stone ◽

Katrin Kellner ◽

Jon N. Seal

Keyword(s):

Microsatellite Markers ◽

North American ◽

Polymorphic Markers ◽

Model Group ◽

Cross Amplification ◽

Attine Ants ◽

Amplification Success ◽

Genetic Patterns ◽

Polymorphic Microsatellite ◽

Generation Sequencing

Abstract Objective The objective of this study is to develop and identify polymorphic microsatellite markers for fungus-gardening (attine) ants in the genus Trachymyrmex sensu lato . These ants are important ecosystem engineers and have been a model group for understanding complex symbiotic systems, but very little is understood about the intraspecific genetic patterns across most North American attine species. These microsatellite markers will help to better study intraspecific population genetic structure, gene flow, mating habits, and phylogeographic patterns in these species and potentially other congeners. Results Using next-generation sequencing techniques, we identified 17 and 12 polymorphic microsatellite loci from T. septentrionalis and Mycetomoellerius (formerly Trachymyrmex ) turrifex , respectively, and assessed the genetic diversity of each locus. We also analyzed the cross-amplification success of the T. septentrionalis markers in two other closely related Trachymyrmex species, and identified 10 and 12 polymorphic markers for T. arizonensis and T. pomonae , respectively.

Download Full-text

Development, characterization, and cross-amplification of polymorphic microsatellite markers for North American Trachymyrmex and Mycetomoellerius ants

BMC Research Notes ◽

10.1186/s13104-020-05015-3 ◽

2020 ◽

Vol 13 (1) ◽

Author(s):

Alix E. Matthews ◽

Chase Rowan ◽

Colby Stone ◽

Katrin Kellner ◽

Jon N. Seal

Keyword(s):

Microsatellite Markers ◽

North American ◽

Cross Amplification ◽

Polymorphic Microsatellite

Download Full-text

Microsatellite development via next-generation sequencing in Acacia stenophylla (Fabaceae) and Duma florulenta (Polygonaceae): two ecologically important plant species of Australian dryland floodplains

10.7287/peerj.preprints.27027v1 ◽

2018 ◽

Author(s):

Bruce F Murray ◽

Michael A Reid ◽

Shu-Biao Wu

Keyword(s):

Gene Flow ◽

Next Generation Sequencing ◽

Microsatellite Markers ◽

Information Content ◽

Polymorphic Information Content ◽

Extreme Variability ◽

Polymorphic Microsatellite ◽

Generation Sequencing ◽

Semi Arid

Duma florulenta and Acacia stenophylla are two ecologically important but understudied species that naturally occur on the floodplains and riverbanks of Australia’s arid and semi-arid river systems. This paper describes the discovery and characterization of 12 and 13 polymorphic microsatellite markers for D. florulenta and A. stenophylla respectively. The number of alleles per locus for D. florulenta ranged from 2-12 with an average of 6.1. Across all samples, observed and expected heterozygosities ranged from 0.026 to 0.784 and 0.026 to 0.824 respectively and mean polymorphic information content was equal to 0.453. For A. stenophylla, the number of alleles per locus ranged between 2 and 8 with an overall mean of 4.8. Across all samples, observed and expected heterozygosities ranged from 0.029 to 0.650 and 0.029 to 0.761 respectively and mean polymorphic information content was 0.388. The developed suites of 12 and 13 microsatellite markers for D. florulenta and A. stenophylla respectively provide opportunity for novel research into mechanisms of gene flow, dispersal and breeding system and how they operate under the extreme variability these species are exposed to in the environments in which they live.

Download Full-text