scholarly journals Implanted subcutaneous versus intraperitoneal bioscaffold seeded with hepatocyte-like cells: functional evaluation

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Amal Elham Fares ◽  
Hala Gabr ◽  
Asmaa Mohammed ShamsEldeen ◽  
Haithem A. M. Farghali ◽  
Mazen Mohamed Salem Mohamed Rizk ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hemophilia A ◽  
Subcutaneous Tissue ◽  
Human Hepatocytes ◽  
Cytokeratin 19 ◽  
Functional Evaluation ◽  
Human Stem Cells ◽  
Subcutaneous Implantation ◽  
Line Of Therapy ◽  
Decellularized Liver

Abstract Background and objectives The X-linked bleeding disorder, hemophilia A, is caused by defective production of factor VIII (FVIII). Hemophilic patients require regular FVIII infusions. Recombinant factor replacement poses the safest line of therapy. However, its main drawbacks are high expenses and the higher liability for formation of inhibitors. Recent studies confirmed the ability of bone marrow-derived stem cells to secrete FVIII. This study aims to generate bioscaffold from decellularized liver and subsequently seed it with trans-differentiated human stem cells into hepatic-like cells. This scaffold can then be implanted intraperitoneally or subcutaneously to provide FVIII. Methods After generation of the bioscaffold, seeding of discoid scaffolds with trans-differentiated human hepatocyte-like cells was performed. Then, the generated organoid was implanted into peritoneal cavity or subcutaneous tissue of experimental rats. Results Serum human FVIII was significantly increased in rats subjected to subcutaneous implantation compared intraperitoneal implantation. Immunostaining for detecting Cytokeratin 19 and human anti-globulin confirmed the presence of mature human hepatocytes that were significantly increased in subcutaneous implanted scaffold compared to the intraperitoneal one. Conclusion Implantation of decellularized bioscaffold seeded with trans-differentiated stem cells in rats was successful to establish production of FVIII. Subcutaneous implantation showed higher FVIII levels than intraperitoneal implantation.

Testing Human Stem Cells in Primates

2005 ◽  
Vol 35 (16) ◽  
pp. 14
Author(s):  
GUY McKHANN
Keyword(s):  
Stem Cells ◽  
Human Stem Cells

Human stem cells transplantation to the subretinal space of the retina in the experiment

2020 ◽  
pp. 259-260
Author(s):  
A.A. Mikaelyan ◽  
◽  
N.L. Sheremet ◽  
A.Y. Andreev ◽  
A.A. Plyukhova ◽  
...  
Keyword(s):  
Stem Cells ◽  
Subretinal Space ◽  
Human Stem Cells ◽  
Stem Cells Transplantation

Quantitative Evaluation of Chromosomal Rearrangements in Primary Gene-Edited Human Stem Cells by Preclinical CAST-Seq

2020 ◽  
Author(s):  
Giandomenico Turchiano ◽  
Geoffroy Andrieux ◽  
Georges Blattner ◽  
Valentina Pennucci ◽  
Julia Klermund ◽  
...  
Keyword(s):  
Stem Cells ◽  
Quantitative Evaluation ◽  
Chromosomal Rearrangements ◽  
Human Stem Cells ◽  
Primary Gene

scholarly journals Subcutaneous Implantation Assessment of New Calcium-Silicate Based Sealer for Warm Obturation

Biomedicines ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 24
Author(s):  
João Miguel Santos ◽  
Carolina M. Coelho ◽  
Diana B. Sequeira ◽  
Joana A. Marques ◽  
Joana F. Pereira ◽  
...  
Keyword(s):  
Inflammatory Reaction ◽  
Calcium Silicate ◽  
Subcutaneous Tissue ◽  
Fibrous Capsule ◽  
Subcutaneous Implantation ◽  
Mineralization Potential ◽  
Parametric Data ◽  
Mineralized Tissues ◽  
Ah Plus ◽  
Post Implantation

Calcium silicate-based sealers were recently introduced as a new class of endodontic sealers, with potential further benefits due to their bioactivity. The aim of this study was to evaluate the biocompatibility of two new hydraulic calcium silicate-based sealers, TotalFill BC Sealer (FKG, La Chaux-des-Fonds, Switzerland) and TotalFill BC Sealer HiFlow (FKG, La Chaux-des-Fonds, Switzerland) through subcutaneous implantation in connective tissue of rats. Subcutaneous implantation was performed in 16 young Wistar rats. Four polyethylene tubes were implanted in each animal, one empty to serve as a control, and three filled with tested sealers: AH Plus as reference (Dentsply DeTrey, Konstanz, Germany), TotalFill BC Sealer (BC) and TotalFill BC Sealer HiFlow (HiFlow). Eight rats were euthanized at 8 days and the remaining eight at 30 days. Hematoxylin-eosin staining was used to score the inflammatory reaction, macrophage infiltrate and to measure the thickness of the fibrous capsule. von Kossa staining was performed to evaluate the mineralization level. Kruskal–Wallis test followed by Dunn’s post hoc test was used to analyze non-parametric data. To analyze the influence of the implantation time within each material, a Mann–Whitney U test was performed. At eight days post-implantation, AH Plus induced a more intense inflammatory reaction when compared both with the control (p ≤ 0.001) and BC (p ≤ 0.01). HiFlow presented a higher score of macrophage infiltrate than control (p ≤ 0.01) and BC (p ≤ 0.05). The fibrous capsule thickness in this period was significantly higher for the BC group when compared to control (p ≤ 0.01) and AH Plus (p ≤ 0.05). The mineralization potential was higher for the HiFlow group when compared with the control (p ≤ 0.001) and AH Plus (p ≤ 0.001). At 30 days post-implantation, the score for the inflammatory reaction remained higher for the AH Plus group when compared both to control (p ≤ 0.01) and BC (p ≤ 0.001). The macrophage infiltrate of the HiFlow was significantly higher than control (p ≤ 0.001) and AH Plus groups (p ≤ 0.01), additionally, the fibrous capsule of the BC (p ≤ 0.001) and HiFlow (p ≤ 0.01) groups were both thicker than control. Mineralization potential was observed only on BC (p ≤ 0.05) and HiFlow groups (p ≤ 0.001), when compared to control). BC exhibited the best biocompatibility performance of all tested sealers and HiFlow provided the greatest induction of mineralized tissues. Both TotalFill BC Sealer and TotalFill BC Sealer HiFlow are biocompatible and show potential bioactivity when implanted in the subcutaneous tissue. Bioactivity was not found in AH Plus.

Regeneration of pulp-dentin complex using human stem cells of the apical papilla: in vivo interaction with two bioactive materials

2021 ◽  
Author(s):  
Diana B. Sequeira ◽  
Ana Rafaela Oliveira ◽  
Catarina M. Seabra ◽  
Paulo J. Palma ◽  
Carlos Ramos ◽  
...  
Keyword(s):  
Stem Cells ◽  
Human Stem Cells ◽  
Bioactive Materials ◽  
Apical Papilla

scholarly journals Novel hybrid three-dimensional artificial liver using human induced pluripotent stem cells and a rat decellularized liver scaffold

2019 ◽  
Vol 10 ◽  
pp. 127-133 ◽  
Author(s):  
Takahito Minami ◽  
Takamichi Ishii ◽  
Kentaro Yasuchika ◽  
Ken Fukumitsu ◽  
Satoshi Ogiso ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Three Dimensional ◽  
Artificial Liver ◽  
Induced Pluripotent ◽  
Decellularized Liver

scholarly journals Telomere length set point regulation in human pluripotent stem cells critically depends on the shelterin protein TPP1

2020 ◽  
Vol 31 (23) ◽  
pp. 2583-2596
Author(s):  
John M. Boyle ◽  
Kelsey M. Hennick ◽  
Samuel G. Regalado ◽  
Jacob M. Vogan ◽  
Xiaozhu Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Telomere Length ◽  
Pluripotent Stem Cells ◽  
Human Stem Cells ◽  
Set Point ◽  
Hypomorphic Allele ◽  
Point Control ◽  
Set Point Control ◽  
Stem Cell Lines

To better understand telomere length set point control in human stem cells, we generated knockout stem cell lines for TPP1 and contrasted their phenotypes with those of homozygous TPP1 L104A mutant stem cells. This comparison reveals that TPP1 L104A is not a hypomorphic allele but formally establishes TPP1 L104 as a dissociation of function mutant.

scholarly journals Cellular autophagy of human stem cells in the process of differentiation

2015 ◽  
Vol 26 (2) ◽  
pp. S21
Author(s):  
Shing-Hwa Lu ◽  
Hsin-Chen Lee ◽  
Alex T.L. Lin ◽  
Kuang-Kuo Chen ◽  
Luke S. Chang
Keyword(s):  
Stem Cells ◽  
Human Stem Cells ◽  
Cellular Autophagy
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