scholarly journals Sertoli cell-mediated differentiation of male germ cell-like cells from human umbilical cord Wharton’s jelly-derived mesenchymal stem cells in an in vitro co-culture system

Author(s):  
Lichun Xie ◽  
Limin Lin ◽  
Qiuliu Tang ◽  
Weizhong Li ◽  
Tianhua Huang ◽  
...  
2019 ◽  
Vol 130 ◽  
pp. 8-18
Author(s):  
M.N. Segunda ◽  
J. Bahamonde ◽  
I. Muñoz ◽  
S. Sepulveda ◽  
J. Cortez ◽  
...  

2009 ◽  
pp. n/a-n/a ◽  
Author(s):  
Peng Huang ◽  
Li Min Lin ◽  
Xiao Ying Wu ◽  
Qiu Ling Tang ◽  
Xue Yong Feng ◽  
...  

2021 ◽  
Vol 23 (2) ◽  
pp. 81-86
Author(s):  
Ali Shojaeian ◽  
Ameneh Mehri-Ghahfarrokhi ◽  
Shima Rahmati-Dehkordi ◽  
Mehdi Banitalebi-Dehkordi

Background and aims: Infertility is one of the most common problems among couples. Generation of male germ cells from adult stem cells is a current promising priority of researchers. This study aimed to investigate the potential of human umbilical cord mesenchymal stem cells (hUMSCs) on the expression of male germ cell markers after isolating by this method. Methods: The hUMSCs was incubated with retinoic acid, testosterone, and conditioned medium (prepared from testicular cell cultures of 7-day-old mice) during 3 days. The bands were visualized and densitometry was accomplished using LI-COR Biosciences software. Results: The high expression levels of C-KIT, DAZL, PIWIL2, and DDX4 in mRNA and protein levels were observed in treated hUMSCs. Conclusion: Results of reverse transcription polymerase chain reaction (RT-PCR) and western blotting showed that method of isolation had no adverse effects on differentiation potential of hUMSCs.


2021 ◽  
Vol 11 (7) ◽  
Author(s):  
Zhao Ting ◽  
Yan Zhi‐xin ◽  
Tan You‐wen ◽  
Yang Fu‐ji ◽  
Sun Hui ◽  
...  

2012 ◽  
Vol 21 (18) ◽  
pp. 3289-3297 ◽  
Author(s):  
Hong-Chao Zhang ◽  
Xin-Bin Liu ◽  
Shu Huang ◽  
Xiao-Yun Bi ◽  
Heng-Xiang Wang ◽  
...  

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