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Published By Springer-Verlag

1869-1889, 1674-7305

Author(s):  
Xin Huang ◽  
Huika Xia ◽  
Qi Zhang ◽  
Colin Blakemore ◽  
Yan Nan ◽  
...  

Author(s):  
Fang He ◽  
Yuan Liu ◽  
Pan Li ◽  
Xiaoyan Wu ◽  
Yaoyao Xia ◽  
...  

Author(s):  
Wei Xiao ◽  
Zhuo-Lei Jiao ◽  
Esra Senol ◽  
Jiwei Yao ◽  
Miao Zhao ◽  
...  

Author(s):  
Jianting Zhou ◽  
Chao Zhang ◽  
Ran Wei ◽  
Mingzhe Han ◽  
Songduo Wang ◽  
...  

AbstractYeast artificial chromosomes (YACs) are important tools for sequencing, gene cloning, and transferring large quantities of genetic information. However, the structure and activity of YAC chromatin, as well as the unintended impacts of introducing foreign DNA sequences on DNA-associated biochemical events, have not been widely explored. Here, we showed that abundant genetic elements like TATA box and transcription factor-binding motifs occurred unintentionally in a previously reported data-carrying chromosome (dChr). In addition, we used state-of-the-art sequencing technologies to comprehensively profile the genetic, epigenetic, transcriptional, and proteomic characteristics of the exogenous dChr. We found that the data-carrying DNA formed active chromatin with high chromatin accessibility and H3K4 tri-methylation levels. The dChr also displayed highly pervasive transcriptional ability and transcribed hundreds of noncoding RNAs. The results demonstrated that exogenous artificial chromosomes formed chromatin structures and did not remain as naked or loose plasmids. A better understanding of the YAC chromatin nature will improve our ability to design better data-storage chromosomes.


Author(s):  
Lina Li ◽  
Canxing Duan ◽  
Jianfeng Weng ◽  
Xiantao Qi ◽  
Changlin Liu ◽  
...  
Keyword(s):  

Author(s):  
Ling-Yan Su ◽  
Yuan Li ◽  
Qianjin Liu ◽  
Lijin Jiao ◽  
Jing Shen ◽  
...  

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