scholarly journals Evaluation of isolation method in remaining of differentiation potential of perivascular human umbilical cord mesenchymal stem cells toward male germ cell-like

2021 ◽  
Vol 23 (2) ◽  
pp. 81-86
Author(s):  
Ali Shojaeian ◽  
Ameneh Mehri-Ghahfarrokhi ◽  
Shima Rahmati-Dehkordi ◽  
Mehdi Banitalebi-Dehkordi
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Germ Cell ◽  
Umbilical Cord ◽  
Adult Stem Cells ◽  
Male Germ Cell ◽  
Human Umbilical Cord ◽  
Differentiation Potential ◽  
Protein Levels ◽  
Old Mice

Background and aims: Infertility is one of the most common problems among couples. Generation of male germ cells from adult stem cells is a current promising priority of researchers. This study aimed to investigate the potential of human umbilical cord mesenchymal stem cells (hUMSCs) on the expression of male germ cell markers after isolating by this method. Methods: The hUMSCs was incubated with retinoic acid, testosterone, and conditioned medium (prepared from testicular cell cultures of 7-day-old mice) during 3 days. The bands were visualized and densitometry was accomplished using LI-COR Biosciences software. Results: The high expression levels of C-KIT, DAZL, PIWIL2, and DDX4 in mRNA and protein levels were observed in treated hUMSCs. Conclusion: Results of reverse transcription polymerase chain reaction (RT-PCR) and western blotting showed that method of isolation had no adverse effects on differentiation potential of hUMSCs.

EMERGING LEADER IN STEM CELL THERAPY: HUMAN UMBILICAL CORD MESENCHYMAL STEM CELLS-FUTURE THERAPEUTIC TRENDS

2021 ◽  
pp. 19-21
Author(s):  
Pradeep Kumar Radhakrishnan ◽  
Roshini Ambat ◽  
Sushamma Vikraman ◽  
Geetha Nagasree N ◽  
Hariharan Hariharan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Adult Stem Cells ◽  
Ethical Issues ◽  
Therapeutic Potential ◽  
Embryonic Stem ◽  
Easy Access ◽  
Human Umbilical Cord ◽  
Differentiation Potential

Mesenchymal stem cells (MSCs) are multipotent adult stem cells widely distributed in the bone marrow, umbilical cord, fat, and other tissues and have high proliferation, multi-differentiation, and immunoregulatory abilities. They can inhibit the proliferation of immune cells and the secretion of inammatory factors [26]. Compared with MSCs from other sources, human umbilical cord MSCs (hUCMSCs) have many advantages, such as a wide source, easy access to materials, strong proliferation ability, low immunogenicity, and great differentiation potential. They are most likely to become pluripotent stem cells with clinical application prospects. Wharton's jelly mesenchymal stem cells – WJMSC- provide three classic advantages – ease of collection with no legal or ethical issues, high differentiating potential and low immunogenicity. Shorter doubling time (21) and an extensive ex vivo expansion capacity provides yet another privileged status to these cells compared with embryonic stem cells. Therapeutic potential of these cells lie in their immuno-modulatory properties involving both innate and adaptive immunity. Graft vs Host disease (GvHD), Post transplant scenarios and autoimmune disorders could witness a revolution in treatment approach with greater understanding of the mechanism action of these cells. Regenerative medicine should get an immense benet from proper understanding and utilization of these cells.

scholarly journals Differentiation of Human Umbilical Cord Derived Mesenchymal Stem Cells Into Neural Stem Cells Induced by hPRDX5

2021 ◽  
Author(s):  
Yuanyuan Jin ◽  
Beichen Shi ◽  
Qiang Fan ◽  
Kun Liu ◽  
Shuai Fan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Neural Stem Cells ◽  
Umbilical Cord ◽  
Expression Profiles ◽  
Antigen Expression ◽  
Culture Method ◽  
Human Umbilical Cord ◽  
Differentiation Potential ◽  
Surface Antigen Expression

Abstract Background: The objectives of this study were to investigate the characteristics and capacity of human umbilical cord‑derived mesenchymal stem cells (hUC-MSCs) differentiation into neural stem cells (NSCs) and whether this event enhanced by hPRDX5. Methods and Results: The adherent cells were obtained from umbilical cord of normal full-term newborn by caesarean section under aseptic condition, and cultivated by tissue block culture method. The surface antigen expression profiles of hUC-MSCs were monitored and the multi-directional differentiation potential was identified. Following amplification, the cells of the 4th passage were divided into 5 groups (groups A-E). The morphology was observed under inverted microscope, and the positive expression rate of markers of neural stem cell was detected by immunocytochemical and western blot. Flow cytometry revealed that the hUC-MSCs expressed CD29, CD73, CD90 and CD105, but not CD19, CD34, CD45 or HLA-DR. Treatment with hPRDX5 led to the surface markers of neural stem cells which were positive for Nestin, but negative for NSE and GFAP expression. Conclusions: Thus, the findings of the present study demonstrate that hPRDX5 effectively promotes hUC-MSCs to differentiate into neural stem cells possibly through TLR4 signaling pathway.

scholarly journals The subpopulation of CD105 negative mesenchymal stem cells show strong immunomodulation capacity compared to CD105 positive mesenchymal stem cells

2019 ◽  
Vol 6 (4) ◽  
pp. 3131-3140 ◽  
Author(s):  
Liem Hieu Pham ◽  
Ngoc Bich Vu ◽  
Phuc Van Pham
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Umbilical Cord ◽  
Immune Modulation ◽  
Human Mesenchymal Stem Cells ◽  
Human Adipose Tissue ◽  
Human Umbilical Cord ◽  
Differentiation Potential

Introduction: Human mesenchymal stem cells (MSCs) are the most popular stem cells applied in disease treatment. MSCs can be isolated and in vitro expanded from various sources such as bone marrow, peripheral blood, umbilical cord blood, umbilical cord tissue, and adipose tissue. According to Dominici et al. (2006), MSCs should express CD105, an essential marker used to confirm MSCs. However, some recent studies have show that MSCs contained a subpopulation that is negative for CD105. This study aimed to compare the immune modulation capacity of 2 populations of CD105 positive (CD105+) and negative (CD105-) MSCs derived from 2 sources: human adipose tissue (AT) and human umbilical cord (UC). Methods: MSCs were isolated from human adipose tissues (adipose tissue-derived mesenchymal stem cells – AT-MSCs) and human umbilical cord (umbilical cord-derived mesenchymal stem cells – UC-MSCs) according to previously published protocols. The two populations of CD105- and CD105+ MSCs were sorted based on the expression of CD105 from AT-MSCs and UC-MSCs. Four populations of CD105 (AT-MSCs, CD105+ AT-MSCs, CD105- UC-MSCs, and CD105+ UC-MSCs) were used to compare the phenotype as well as in vitro differentiation potential; then they were used to evaluate the immune modulation capacity by allogeneic T cell suppression and cytokine release. Results: The results showed that CD105- MSCs from AT and UC exhibited an immune modulation capacity that was much stronger than CD105+ MSCs from the same source of AT and UC. The strong immunomodulation of CD105- MSCs may relate to autocrine production of TGF-beta 1 by MSCs. Conclusion: The results suggested that CD105- MSCs are promising MSCs for application in regenerative medicine, especially for the treatment of diseases related to inflammation.  

scholarly journals Intra-articular injection of human umbilical cord mesenchymal stem cells ameliorates monosodium iodoacetate-induced osteoarthritis in rats by inhibiting cartilage degradation and inflammation

2021 ◽  
Vol 10 (3) ◽  
pp. 226-236
Author(s):  
Quan Zhang ◽  
E. Xiang ◽  
Wei Rao ◽  
Ya Qi Zhang ◽  
Cui Hong Xiao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Articular Cartilage ◽  
Umbilical Cord ◽  
Cartilage Degradation ◽  
Human Umbilical Cord ◽  
Differentiation Potential ◽  
Tnf Α ◽  
Monosodium Iodoacetate ◽  
Safranin O

Aims This study aimed to investigate whether human umbilical cord mesenchymal stem cells (UC-MSCs) can prevent articular cartilage degradation and explore the underlying mechanisms in a rat osteoarthritis (OA) model induced by monosodium iodoacetate (MIA). Methods Human UC-MSCs were characterized by their phenotype and multilineage differentiation potential. Two weeks after MIA induction in rats, human UC-MSCs were intra-articularly injected once a week for three weeks. The therapeutic effect of human UC-MSCs was evaluated by haematoxylin and eosin, toluidine blue, Safranin-O/Fast green staining, and Mankin scores. Markers of joint cartilage injury and pro- and anti-inflammatory markers were detected by immunohistochemistry. Results Histopathological analysis showed that intra-articular injection of human UC-MSCs significantly inhibited the progression of OA, as demonstrated by reduced cartilage degradation, increased Safranin-O staining, and lower Mankin scores. Immunohistochemistry showed that human UC-MSC treatment down-regulated the expression of matrix metalloproteinase-13 (MMP13) and a disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS-5), and enhanced the expression of type II collagen and ki67 in the articular cartilage. Furthermore, human UC-MSCs significantly decreased the expression of interleukin (IL)-1β and tumour necrosis factor-α (TNF-α), while increasing TNF-α-induced protein 6 and IL-1 receptor antagonist. Conclusion Our results demonstrated that human UC-MSCs ameliorate MIA-induced OA by preventing cartilage degradation, restoring the proliferation of chondrocytes, and inhibiting the inflammatory response, which implies that human UC-MSCs may be a promising strategy for the treatment of OA. Cite this article: Bone Joint Res 2021;10(3):226–236.

scholarly journals Transdifferentiation of Human Umbilical Cord- Derived Mesenchymal Stem Cells in Dopaminergic Neurons in a Three-Dimensional Culture

2021 ◽  
pp. 1-25
Author(s):  
Ardeshir Moayeri ◽  
◽  
Rafieh Alizadeh ◽  
Hatef Ghasemi Hamidabadi ◽  
Maryam Nazm Bojnordi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Dopaminergic Neurons ◽  
Dopaminergic Neuron ◽  
High Performance ◽  
Three Dimensional ◽  
Human Umbilical Cord ◽  
Neuronal Markers ◽  
Protein Levels

Introduction: The induction of from human umbilical cord-derived mesenchymal stem cells (HUC-MSCs) toward dopaminergic neurons is a major challenge in tissue engineering and experimental and clinical treatments of various neurodegenerative diseases including Parkinson’s disease. This study aimed to differentiate HUC-MSCs into dopaminergic neuron-like cells. Methods: After HUC-MSCs isolation and characterizations, they transferred onto matrigel- coated plates and incubated with a cocktail of dopaminergic neuronal differentiation factors. The capacity of differentiation into dopaminergic neuron-like cells in 2D culture and on matrigel was assessed by real-time PCR, immunocytochemistry and high-performance liquid chromatography (HPLC). Results: Our results showed that the transcript and protein levels of dopaminergic neuronal markers was significantly increased on Matrigel differentiated cells as compared with 2D culture plates. Conclusions: Overall, the results of this study suggest that HUC-MSCs can successfully differentiate toward dopaminergic neuron-like cells on Matrigel, having great potentials for the treatment of dopaminergic neuron-related diseases.

scholarly journals ID: 1032 Differentiation Potential and Characteristics of Mesenchymal Stem Cells Isolated from Human Umbilical Cord membrane to Hepatocyte-like Cells

2017 ◽  
Vol 4 (S) ◽  
pp. 107
Author(s):  
Trung Kien Do ◽  
Van Hanh Nguyen ◽  
Huu Duc Nguyen ◽  
Chu Hoang Ha
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Umbilical Cord ◽  
Gene Transfection ◽  
Human Umbilical Cord ◽  
Specific Marker ◽  
Differentiation Potential

Recent studies indicated that Mesenchymal stem cell has become a potential objective for therapy. In this study, umbilical cord cells were isolated and analyzed the expression of mesenchymal stem cells specific markers then they were differentiated into hepatocyte-like cells by DMSO and Gene transfection. Umbilical cord mesenchymal stem cell (MSC) was isolated by explant culture in media DMEM/F12, complementing with growth factors EGF, FGF and IST. After that, they were exposured to DMSO with three concentrations: 0.01%, 0.1%, 1% and another group was transfection with HNF4α by Lipofectamin LX plus. The cells were analyzed at 1, 2, 3, and 4 weeks after treatment. The cells isolation was shown the positive with markers CD73, CD34, CD86, CD90, CD105, eras, Oct 1, GATA, and negative with markers HNF4α, Alb and G6P. In group 0,1% DMSO treatment, after 3 weeks the cells were positive with markers HNF4α but it was also negative with markers Alb and G6P. In the transfection group, the cell expresses HNF4α at three weeks after treatment. Although our results exposure that the umbilical cord mesenchymal stem cells expressed hepatic specific marker after DMSO induced and DNA treatment. So it will be necessary to optimize research conditional and investigate the hepatic functions of these cells in a longer in vitro culture.

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