Safety and long-lasting immunity of the combined administration of a modified-live virus vaccine against porcine reproductive and respiratory syndrome virus 1 and an inactivated vaccine against porcine parvovirus and Erysipelothrix rhusiopathiae in breeding pigs

ABSTRACT This paper provides information on the complete genome sequence of a porcine reproductive and respiratory syndrome virus (PRRSV) strain isolated on a French pig farm which was identified as a recombinant strain from two commercial modified live virus vaccine strains of genotype 1 (VP-046BIS and DV strains).

Download Full-text

Peptide nanofiber hydrogel adjuvanted live virus vaccine enhances cross-protective immunity to porcine reproductive and respiratory syndrome virus

Vaccine ◽

10.1016/j.vaccine.2013.07.080 ◽

2013 ◽

Vol 31 (41) ◽

pp. 4508-4515 ◽

Cited By ~ 28

Author(s):

Xiangdong Li ◽

Amy Galliher-Beckley ◽

Hongzhou Huang ◽

Xiuzhi Sun ◽

Jishu Shi

Keyword(s):

Virus Vaccine ◽

Protective Immunity ◽

Respiratory Syndrome Virus ◽

Live Virus ◽

Live Virus Vaccine

Download Full-text

Commercial porcine reproductive and respiratory syndrome virus (PRRSV)-2 modified live virus vaccine against heterologous single and dual Korean PRRSV-1 and PRRSV-2 challenge

Veterinary Record ◽

10.1136/vr.104397 ◽

2018 ◽

Vol 182 (17) ◽

pp. 485-485 ◽

Cited By ~ 2

Author(s):

Jiwoon Jeong ◽

Seeun Kim ◽

Changhoon Park ◽

Kee Hwan Park ◽

Ikjae Kang ◽

...

Keyword(s):

Nucleic Acid ◽

Virus Vaccine ◽

Interferon Γ ◽

Growing Pigs ◽

Respiratory Syndrome Virus ◽

Live Virus ◽

Lung Lesions ◽

Viral Loads ◽

Live Virus Vaccine ◽

Ifn Γ

This study evaluated porcine reproductive and respiratory syndrome virus (PRRSV)-2 modified live virus (MLV) vaccine against heterologous single and dual challenge of Korean PRRSV-1 and PRRSV-2. Pigs were administered PRRSV-2 MLV vaccine intramuscularly at 21 days of age and inoculated intranasally with both genotypes at 56 days of age. Vaccination of pigs with PRRSV-2 MLV vaccine resulted in reduction of viral loads of both PRRSV-1 and PRRSV-2 after heterologous single and dual challenge with PRRSV-1 and PRRSV-2. In addition, pigs vaccinated with PRRSV-2 MLV vaccine exhibited higher frequencies of PRRSV-1 and PRRSV-2 specific interferon-γ secreting cells (IFN-γ-SC) and showed a significant reduction in lung lesions and PRRSV nucleic acid within the lung lesions after single and dual challenge compared with unvaccinated challenged pigs. Taken together these results demonstrated that vaccination of pigs with PRRSV-2 is efficacious in protecting growing pigs from respiratory disease against heterologous single and dual PRRSV-1 and PRRSV-2 challenge.

Download Full-text

Cross-protective immunity to porcine reproductive and respiratory syndrome virus by intranasal delivery of a live virus vaccine with a potent adjuvant

Vaccine ◽

10.1016/j.vaccine.2011.03.006 ◽

2011 ◽

Vol 29 (23) ◽

pp. 4058-4066 ◽

Cited By ~ 42

Author(s):

Varun Dwivedi ◽

Cordelia Manickam ◽

Ruthi Patterson ◽

Katie Dodson ◽

Michael Murtaugh ◽

...

Keyword(s):

Virus Vaccine ◽

Protective Immunity ◽

Respiratory Syndrome Virus ◽

Intranasal Delivery ◽

Live Virus ◽

Live Virus Vaccine

Download Full-text

Efficacy of a Spanish modified live virus vaccine against homologous porcine reproductive and respiratory syndrome virus infection

Spanish Journal of Agricultural Research ◽

10.5424/sjar/2006043-6299 ◽

2014 ◽

Vol 4 (3) ◽

pp. 213

Author(s):

E. Álvarez ◽

A. Fernández-García ◽

C. Prieto ◽

J. Martínez-Lobo ◽

I. Simarro ◽

...

Keyword(s):

Virus Infection ◽

Virus Vaccine ◽

Respiratory Syndrome Virus ◽

Live Virus ◽

Live Virus Vaccine

Download Full-text

Field evaluation of the safety and compatibility of a combined vaccine against porcine parvovirus 1 and porcine reproductive and respiratory syndrome virus in breeding animals

Porcine Health Management ◽

10.1186/s40813-019-0138-5 ◽

2019 ◽

Vol 5 (1) ◽

Cited By ~ 1

Author(s):

Beatriz Garcia-Morante ◽

Marta Noguera ◽

Christian Kraft ◽

Philip Bridger

Keyword(s):

Field Evaluation ◽

Respiratory Syndrome Virus ◽

Porcine Parvovirus ◽

Productive Performance ◽

Live Virus ◽

Control Programs ◽

Systemic Reactions ◽

Common Causes ◽

Combined Administration ◽

Combined Vaccine

Abstract Background Porcine reproductive and respiratory syndrome virus (PRRSV) and porcine parvovirus 1 (PPV1) are two common causes of reproductive failure. ReproCyc® ParvoFLEX is a novel subunit vaccine based on the protective viral protein (VP) 2 of PPV1 that has been recently licensed in the European (EU) market, whereas ReproCyc® PRRS EU is a porcine reproductive and respiratory syndrome (PRRS) modified live virus (MLV) vaccine authorized in 2015. The present work sought to evaluate the safety and compatibility of the combined administration of the abovementioned vaccines in target animals under the context of a field PRRSV (experiment A) and PPV1 (experiment B) infection. To achieve this objective, safety and lack of vaccines’ antigen interference were established according to the absence of significant differences between the combined vaccinated animals (PPRSV+PPV1) and the single vaccinated animals against PRRSV or PPV1. In both experiments, gilts and sows were evaluated for local and systemic reactions after vaccination as well as for reproductive and productive performance. In addition, tissues from abortions, mummified fetuses and stillborn piglets were analyzed for the presence of PRRSV and PPV1. Lastly, serology and viremia were determined in experiment B. Results No relevant differences in terms of safety, reproductive and productive performance between the single vaccinated and the combined vaccinated animals in either experiment were observed. Conclusions ReproCyc® PRRS EU mixed with ReproCyc® ParvoFLEX can be used as a safe method of protection against the detrimental effects of PRRSV and PPV1 infections in breeding female pigs in one single injection. The present results also open up opportunities to tackle reproductive problems as a whole by combining control programs against swine reproductive pathogens.

Download Full-text

93 Genomic basis of antibody response to porcine reproductive and respiratory syndrome virus vaccination

Journal of Animal Science ◽

10.1093/jas/skaa054.050 ◽

2020 ◽

Vol 98 (Supplement_3) ◽

pp. 28-28

Author(s):

Letícia P Sanglard ◽

Rohan L Fernando ◽

Kent Gray ◽

Daniel C L Linhares ◽

Jack Dekkers ◽

...

Keyword(s):

Virus Vaccine ◽

Genomic Prediction ◽

Fixed Effects ◽

Natural Infection ◽

Chromosome 7 ◽

Validation Dataset ◽

Respiratory Syndrome Virus ◽

Live Virus ◽

Live Virus Vaccine ◽

Genomic Basis

Abstract Antibody (Ab) response to natural infection with porcine reproductive and respiratory syndrome (PRRS) virus has been shown to be highly heritable (~0.40), to be genetically correlated with farrowing performance in PRRSV-infected sows, be controlled by two major QTL on chromosome 7, among others, and have moderate genomic prediction accuracy. However, waiting for PRRS outbreaks to occur to collect data limits the use of Ab response to select for increased PRRS resilience. Thus, we investigated the genomic basis of Ab response to PRRS vaccination with a modified live PRRSV vaccine as a strategy to generate data for this purpose. Nine hundred and six commercial F1 replacement gilts (189±16 days old) were vaccinated with a commercial PRRS modified live virus vaccine. Blood samples were collected 52 days after vaccination to measure Ab response, as sample-to-positive (S/P) ratio using a commercial ELISA, and for SNP genotyping (~50K). BayesC0 was used to estimate heritability for S/P ratio using in a model with contemporary group (CG) as fixed effect and SNP effects as random. Genome-wide association study and genomic prediction for S/P ratio were performed with BayesB (Pi=0.99). For genomic prediction, a three-fold cross-validation was used, in which each CG (n=3) was used as validation dataset. Accuracy of genomic prediction was defined as the correlation between genomic estimated breeding values and phenotypes adjusted for estimates of fixed effects, weighed by the number of individuals in the validation dataset. Heritability of S/P was moderate (0.35±0.04). A QTL was identified on chromosome 7 (25 Mb) explaining ~28% of the genetic variance. Accuracy of genomic prediction was fairly high (0.60±0.15). This is the first study describing the genomic basis of Ab response to PRRS vaccination with modified-live virus vaccine. Additional work is needed to evaluate the genetic correlation of Ab response to vaccination with resilience traits in pigs.

Download Full-text

Effects of Low (Modified-live Virus Vaccine) and High (VR-2385)-Virulence Strains of Porcine Reproductive and Respiratory Syndrome Virus on Pulmonary Clearance of Copper Particles in Pigs

Veterinary Pathology ◽

10.1177/030098589803500509 ◽

1998 ◽

Vol 35 (5) ◽

pp. 398-406 ◽

Cited By ~ 30

Author(s):

R. Thanawongnuwech ◽

P. G. Halbur ◽

M. R. Ackermann ◽

E. L. Thacker ◽

R. L. Royer

Keyword(s):

Virus Vaccine ◽

Copper Phthalocyanine ◽

Lavage Fluid ◽

Serum Copper ◽

Respiratory Syndrome Virus ◽

Control Group ◽

Live Virus ◽

Copper Particles ◽

Live Virus Vaccine ◽

Pulmonary Clearance

Seventy-five 3-week-old, crossbred pigs from a herd free of porcine reproductive and respiratory syndrome virus (PRSSV) were randomly assigned to three groups: uninfected controls, pigs inoculated intranasally with RespPRRS/Repro™ modified-live virus vaccine (RespPRRS), and pigs inoculated intranasally with a high-virulence strain of PRRSV (VR-2385). Pigs were intravenously infused with 3% copper phthalocyanine tetrasulfonic acid (0.2 ml/kg) in normal saline 30 minutes before necropsy, which was performed 3, 7, 10, 14, or 28 days postinoculation (DPI) with PRRSV. There were no differences in serum copper concentration in samples collected at 0, 15, or 30 minutes after infusion. Copper concentrations in the lungs of VR-2385-inoculated pigs were significantly lower than levels in the lungs of control and RespPRRS-inoculated pigs at 7, 10, and 14 DPI ( P < 0.05). The greatest difference between the groups was observed at 10 DPI. Liver and spleen copper concentrations were slightly, but not significantly, higher in both PRRSV-infected groups. The percentage of lung affected by grossly visible pneumonia ranged from 0 to 5.6% in the RespPRRS-inoculated group and from 15.2 to 46.4% in the VR-2385-inoculated group, with lesions peaking at 7 and 10 DPI, respectively. PRRSV antigen was demonstrated in both pulmonary alveolar macrophages (PAMs) and pulmonary intravascular macrophages (PIMs) by immunohistochemical methods. Copper particles were demonstrated in the PIMs by light microscopy. PRRSV was isolated from bronchoalveolar lavage fluid of VR-2385-infected pigs from 3 to 28 DPI and from RespPRRS-inoculated pigs from 7 to 28 DPI. No PRRSV, PRRSV antibodies, or PRRSV-induced pneumonia was detected in the control group. These results suggest that 1) PRRSV has a detrimental effect on the uptake of copper particles by PIMs, 2) the severity of PRRSV-induced damage to PIMs differs among strains, and 3) demonstration of PRRSV-induced decreased pulmonary clearance supports the hypothesis that PRRSV infection may make pigs more susceptible to bacterial septicemia.

Download Full-text