scholarly journals Umbilical cord blood-derived mesenchymal stem cells consist of a unique population of progenitors co-expressing mesenchymal stem cell and neuronal markers capable of instantaneous neuronal differentiation

2012 ◽  
Vol 3 (6) ◽  
pp. 57 ◽  
Author(s):  
Mundackal S Divya ◽  
George E Roshin ◽  
Thulasi S Divya ◽  
Vazhanthodi Rasheed ◽  
Thankayyan R Santhoshkumar ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cord Blood ◽  
Neuronal Differentiation ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Neuronal Markers

Role of Homing Regulation in Coculturing Human Cord blood–derived Mesenchymal Stem cells with CD34-Positive Cells from Umbilical Cord Blood

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 4747-4747
Author(s):  
Mark Lee ◽  
Heesun Hong ◽  
Sung Yong Kim ◽  
Yo Han Cho ◽  
So Young Yoon
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Hematopoietic Stem ◽  
Cd34 Cells

Abstract Background and Objectives Mesenchymal stem cells plays an important role in the hematopoietic stem cell engraftment condition with SDF-1 (CXCL12)-CXCR4 signaling and in their homing in various tissues. In this study, we evaluated that the regulation of homing efficiency for mesenchymal stem cells to support ex vivo expansion of hematopoietic stem cells from umbilical cord blood. Methods We investigated the expression of CXCR4 and Stromal-Derived Factor-1 (SDF-1) in cocultured mesenchymal stem cell with umbilical cord blood-derived CD34-positive cell, which stimulated with granulocyte macrophage-colony stimulating factor (GM-CSF) and stem cell factor (SCF) cytokine. Results In this study, we evaluated that coculturing of SDF-1+ mesenchymal stem cells with stimulated CD34+ cells significantly increased the expression of CD34, CD45, and CD19 for myeloid surface marker and intracellular CXCR4 within a few hours as compared with culturing of CD34-positive cells alone or with SDF-1− mesenchymal stem cells or untreated mesenchymal stem cells by Flow cytometre. In the result of stimulation for 48 hours with various cytokines in CD34-positive cells, CXCR4 gene and ERK-1,2 protein up-regulated, and increased in vitro migration capacity of cocultured SDF-1+ mesenchymal stem cell with CD34+ cells as examined by quantitative RT-PCR of human GAPDH. To enhance homing effect by mesenchymal stem cell, we maintained expanded mesenchymal stem cells for up to 5–10 passages with monitoring of the expression of various tissue surface antigens, such as skeletal muscle, neural, liver, and endothelial cells. SDF-1+ mesenchymal stem cells induced the homing of cellular products of stimulated cord blood-derived CD34-positive cells for 10 days. Moreover, the tranfected SDF-1+ cells with a green fluorescent protein gene using lentivirus maintained their capacities of protein release and homing in culture system. SDF-1− mesenchymal stem cells reduced CXCR4 expression in cocultured CD34-positive cells. Conclusions: These results demonstrate that the role of the SDF-1/CXCR4 axis is an important rold in the regulation of homing and engraftment of mesenchymal and hematopoietic stem cells. SDF-1+ mesenchymal stem cells have clinical potential to regulate homing and short-term engraftment for hematopoietic stem cell transplantation.

scholarly journals A Small-Sized Population of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Shows High Stemness Properties and Therapeutic Benefit

2020 ◽  
Vol 2020 ◽  
pp. 1-17 ◽  
Author(s):  
Miyeon Kim ◽  
Yun Kyung Bae ◽  
Soyoun Um ◽  
Ji Hye Kwon ◽  
Gee-Hye Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Surface Proteins ◽  
Lung Damage ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord

Mesenchymal stem cells (MSCs) represent a promising means to promote tissue regeneration. However, the heterogeneity of MSCs impedes their use for regenerative medicine. Further investigation of this phenotype is required to develop cell therapies with improved clinical efficacy. Here, a small-sized population of human umbilical cord blood-derived MSCs (UCB-MSCs) was isolated using a filter and centrifuge system to analyze its stem cell characteristics. Consequently, this population showed higher cell growth and lower senescence. Additionally, it exhibited diverse stem cell properties including differentiation, stemness, and adhesion, as compared to those of the population before isolation. Using cell surface protein array or sorting analysis, both EGFR and CD49f were identified as markers associated with the small-sized population. Accordingly, suppression of these surface proteins abolished the superior characteristics of this population. Moreover, compared to that with large or nonisolated populations, the small-sized population showed greater therapeutic efficacy by promoting the engraftment potential of infused cells and reducing lung damage in an emphysema mouse model. Therefore, the isolation of this small-sized population of UCB-MSCs could be a simple and effective way to enhance the efficacy of cell therapy.

scholarly journals Intra-Articular Injection of 2 Different Dosages of Autologous and Allogeneic Bone Marrow- and Umbilical Cord-Derived Mesenchymal Stem Cells Triggers a Variable Inflammatory Response of the Fetlock Joint on 12 Sound Experimental Horses

2019 ◽  
Vol 2019 ◽  
pp. 1-17 ◽  
Author(s):  
Lélia Bertoni ◽  
Thomas Branly ◽  
Sandrine Jacquet ◽  
Mélanie Desancé ◽  
Loïc Desquilbet ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Allogeneic Bone Marrow ◽  
Allogeneic Bone ◽  
Significant Difference

Osteoarthritis is a significant and costly cause of pain for both humans and horses. The horse has been identified as a suitable model for human osteoarthritis. Regenerative therapy with allogeneic mesenchymal stem cells (MSCs) is a promising treatment, but the safety of this procedure continues to be debated. The aim of this study is to evaluate the safety of intra-articular injections of allogeneic MSCs on healthy joints by comparing two different dosages and two different tissue sources, namely, bone marrow and umbilical cord blood, with a placebo treatment on the same individuals. We also assessed the influence of autologous versus allogeneic cells for bone marrow-derived MSC treatment. Twelve clinically sound horses were subjected to injections in their 4 fetlock joints. Each of the three fetlocks was administered a different MSC type, and the remaining fetlock was injected with phosphate-buffered saline as a control. Six horses received 10 million cells per joint, and the 6 other horses received 20 million cells per joint. Clinical and ultrasound monitoring revealed that allogeneic bone marrow-derived MSCs induced significantly more synovial effusion compared to umbilical cord blood-derived MSCs but no significant difference was noted within the synovial fluid parameters. The administration of 10 million cells in horses triggered significantly more inflammatory signs than the administration of 20 million cells. Mesenchymal stem cell injections induced mild to moderate local inflammatory signs compared to the placebo, with individual variability in the sensitivity to the same line of MSCs. Understanding the behavior of stem cells when injected alone is a step towards the safer use of new strategies in stem cell therapy, where the use of either MSC secretome or MSCs combined with biomaterials could enhance their viability and metabolic activity.

CELLULAR AND MOLECULAR EVALUATION OF IN VITRO QUALITY PARAMETERS OF HUMAN UMBILICAL CORD BLOOD-DERIVED MESENCHYMAL STEM CELLS

2021 ◽  
pp. 52-54
Author(s):  
Sara Jabeen ◽  
Usha Gupta ◽  
Aleem Ahmed Khan
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Stem Cell Markers ◽  
Number Of Cells

Introduction: Establishing a reproducible adult stem cell culture system, such as mesenchymal stem cells (MSCs), is critical for elucidating the function of molecular markers associated with these cells' undifferentiated state. In this study, we describe some important parameters to be considered for a successful isolation, culture, and characterization of MSCs from human umbilical cord blood (hUCB). Methods: Five hUCB samples were collected from healthy female subjects who were free from infectious diseases and genetic disorders. Mononuclear cells (MNCs) were counted, and viability was determined using MTT assay. MNCs were cultured in DMEM supplemented with 10% fetal bovine serum and enriched through culture and characterized using morphometric, and molecular analysis. Results: The minimum number of cells was 12.5 million and highest number of cells was 20.6 million from all ve samples. In initial culture of MSCs from hUCB, various morphologic phenotypes were seen, although the cells eventually developed a homogeneous broblast-like shape at day 14 showing >80% conuency. Spindle-shaped clonogenic MSCs expressed a high level of CD90, CD105, and CD73, while negative expression of CD34. Our study provided evidence of expansion of enriched MSCs in culture from day 1 to day 21 as supported by data of CD90, CD105 and CD73 expression levels in a time-dependent manner. Conclusion: Our results suggest that the expanded hUCB harbor an enriched source of MSCs that express pluripotent stem cell markers and lack hematopoietic markers after culture and forms the basis for using hUCB as eminent source of MSCs, which can be used for different therapeutic applications.

Estrogen stimulates the neuronal differentiation of human umbilical cord blood mesenchymal stem cells (CD34???)

Neuroreport ◽  
2007 ◽  
Vol 18 (1) ◽  
pp. 35-38 ◽  
Author(s):  
Ji Hye Kang ◽  
Chae Kwan Lee ◽  
Ju Ran Kim ◽  
Seong Jin Yu ◽  
Jong Hee Jo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Neuronal Differentiation ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord

Factors influencing yield and neuronal differentiation of mesenchymal stem cells from umbilical cord blood and matrix

2016 ◽  
Vol 11 (5) ◽  
pp. 465-474 ◽  
Author(s):  
Günther A Rezniczek ◽  
Sandra Kumbruch ◽  
Jan Scheich ◽  
Arne Jensen ◽  
Clemens B Tempfer
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Neuronal Differentiation ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Factors Influencing

Stem cell proteomes: A profile of human mesenchymal stem cells derived from umbilical cord blood

2005 ◽  
Vol 26 (14) ◽  
pp. 2749-2758 ◽  
Author(s):  
Robert E. Feldmann ◽  
Karen Bieback ◽  
Martin H. Maurer ◽  
Armin Kalenka ◽  
Heinrich F. Bürgers ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Human Mesenchymal Stem Cells
Sign in / Sign up

Export Citation Format

Share Document