Proteolytic Activity Demonstrated by Film In Situ Zymography (FIZ): A Clinically Applicable Double-Staining Method Also Involving ImmunoGold-Silver Staining

2004 ◽  
pp. 267-274
Keyword(s):  
Proteolytic Activity ◽  
Silver Staining ◽  
Staining Method ◽  
Double Staining ◽  
Film In Situ Zymography ◽  
Double Staining Method ◽  
In Situ Zymography

scholarly journals Combined beta-galactosidase and immunogold/silver staining for immunohistochemistry and DNA in situ hybridization.

1990 ◽  
Vol 38 (3) ◽  
pp. 325-329 ◽  
Author(s):  
W van den Brink ◽  
C van der Loos ◽  
H Volkers ◽  
R Lauwen ◽  
F van den Berg ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Silver Staining ◽  
Staining Method ◽  
Double Staining ◽  
Reaction Products ◽  
Silver Enhancement ◽  
Silver Staining Method ◽  
Double Staining Method ◽  
Beta Galactosidase

A combination of beta-galactosidase enzyme and the immunogold/silver staining method was studied for evaluation of double-staining experiments. Applications are shown for immunohistochemical double staining using two monoclonal antibodies and for combined immunohistochemistry and DNA in situ hybridization in one tissue section. The following advantages for the present double-staining method were evaluated: superior sensitivity of the immunogold/silver staining method for at least one epitope, which also allows detection of biotinylated DNA probes. The structure of the indolyl precipitate after revelation of beta-galactosidase activity did not show a concealing effect during a sequential double-staining method, as compared with the visualization of peroxidase with diaminobenzidine. These factors, and the sharply contrasting colored reaction products of beta-galactosidase (blue-green) and the immunogold/silver staining method including silver enhancement (brown-black), allow clear distinction of mixed-stained cell constituents.

A Double Staining Method for Histone H3 mRNA and p53 Protein in Oral Tumors Using In Situ Hybridization and Immunohistochemistry.

1999 ◽  
Vol 32 (4) ◽  
pp. 327-332 ◽  
Author(s):  
Tetsunari Nishikawa ◽  
Shoichi Arai ◽  
Kenichi Uobe ◽  
Masahiro Wato ◽  
Kazuya Tominaga ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Staining Method ◽  
P53 Protein ◽  
Histone H3 ◽  
Double Staining ◽  
Double Staining Method ◽  
Oral Tumors

Enzyme histochemical application and backscattered electron imaging to visualize the distribution of lymphatic capillaries

1990 ◽  
Vol 48 (3) ◽  
pp. 880-881
Author(s):  
Seiji Kato
Keyword(s):  
Staining Method ◽  
Double Staining ◽  
Reaction Products ◽  
Blood Capillaries ◽  
Backscattered Electron Imaging ◽  
Histochemical Observation ◽  
Backscattered Electron ◽  
Double Staining Method ◽  
Electron Imaging ◽  
Cryostat Sections

Previously, the author repeatedly confirmed the higher 5’-nucleotidase (5’-Nase) and lower alkaline phoaphatase (ALPase) activities in the wall of lymphatic capillaries reacted with the lead-based method relative to those of blood capillaries. The ALPase, on the other hand, is markedly higher in blood capillaries than in lymphatics. On the basis of these enzyme characteristics, the author has developed a 5’-Nase— ALPase double staining method to differentiate small lymphatics from blood capillaries at the level of the light microcsopy. Furthermore, we applied it to histochemical observation of the lead-containing reaction products of 5’-Nase in lymphatics on the same or adjacent cryostat sections using backscattered electron imaging (BEI) in scanning electron microscope (SEM). This paper presents a new applicability of 5’-Nase histochemistry by BEI-SEM to demonstrate the distribution of lymphatic capillaries in tissue blocks.

scholarly journals Immunohistochemical/histochemical double staining method in the study of the columnar metaplasia of the oesophagus

2014 ◽  
Vol 58 (1) ◽  
Author(s):  
D. Cabibi ◽  
A.G. Giannone ◽  
C. Mascarella ◽  
C. Guarnotta ◽  
M. Castiglia ◽  
...  
Keyword(s):  
Staining Method ◽  
Double Staining ◽  
Columnar Metaplasia ◽  
Double Staining Method

Evaluation of matrix metalloproteinase-2 (mmp-2) activity with film in situ zymography for improved cytological diagnosis of Breast tumors

Breast Cancer ◽  
2006 ◽  
Vol 13 (3) ◽  
pp. 272-278 ◽  
Author(s):  
Akira Fujiwara ◽  
Eri Shibata ◽  
Hitomi Terashima ◽  
Akemi Shishido ◽  
Junko Nishiki ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Breast Tumors ◽  
Matrix Metalloproteinase 2 ◽  
Cytological Diagnosis ◽  
Film In Situ Zymography ◽  
In Situ Zymography

Identification of anti-golgi antibody by double-staining method with antibody to β-cop

1993 ◽  
Vol 6 (1) ◽  
pp. 77
Author(s):  
Hong-Shang Hong ◽  
Yu-Shung Lee ◽  
Heng-Leong Chan ◽  
Tseng-tong Kuo
Keyword(s):  
Staining Method ◽  
Double Staining ◽  
Double Staining Method

scholarly journals Immunocytochemical localization of the posterior lobe hormones and of their carrier proteins.

1980 ◽  
Vol 28 (5) ◽  
pp. 469-471 ◽  
Author(s):  
F Vandesande ◽  
K Dierickx ◽  
N Goossens
Keyword(s):  
Staining Method ◽  
Posterior Lobe ◽  
Serial Sections ◽  
Double Staining ◽  
Carrier Proteins ◽  
Tissue Sections ◽  
Tissue Antigens ◽  
Staining Methods ◽  
Double Staining Method ◽  
The One

Serial sections of vertebrate hypothalami were stained with the immunocytochemical peroxidase-antiperoxidase method. In addition to the single staining method, our double staining method was used, which enabled us to visualize two tissue antigens in single tissue sections. In both staining methods, differentially adsorbed antineurohypophysial hormone sera, anti-somatostatin serum and anti-bovine neurophysin sera were used. The results confirm the one hormone, one neuron hypothesis.

Results of a double staining method for DNA and RNA

1968 ◽  
Vol 55 (10) ◽  
pp. 496-497 ◽  
Author(s):  
Sibdas Ghosh ◽  
Renate Lettr�
Keyword(s):  
Staining Method ◽  
Double Staining ◽  
Dna And Rna ◽  
Double Staining Method
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