Immunohistochemical/histochemical double staining method in the study of the columnar metaplasia of the oesophagus

Previously, the author repeatedly confirmed the higher 5’-nucleotidase (5’-Nase) and lower alkaline phoaphatase (ALPase) activities in the wall of lymphatic capillaries reacted with the lead-based method relative to those of blood capillaries. The ALPase, on the other hand, is markedly higher in blood capillaries than in lymphatics. On the basis of these enzyme characteristics, the author has developed a 5’-Nase— ALPase double staining method to differentiate small lymphatics from blood capillaries at the level of the light microcsopy. Furthermore, we applied it to histochemical observation of the lead-containing reaction products of 5’-Nase in lymphatics on the same or adjacent cryostat sections using backscattered electron imaging (BEI) in scanning electron microscope (SEM). This paper presents a new applicability of 5’-Nase histochemistry by BEI-SEM to demonstrate the distribution of lymphatic capillaries in tissue blocks.

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Interaction between cholinergic neurons and peptidergic or adrenergic fibers in the rat spinal autonomic neurons: Using a new immuno-electron double-staining method for correlated light and electron microscopic observations

Neuroscience Research Supplements ◽

10.1016/0921-8696(88)90078-3 ◽

1988 ◽

Vol 7 ◽

pp. S32

Author(s):

Johji Kohno ◽

Koh Shinoda ◽

Peng Yuwen ◽

Yoshinori Kawai ◽

Keiro Ono ◽

...

Keyword(s):

Staining Method ◽

Cholinergic Neurons ◽

Double Staining ◽

Electron Microscopic ◽

Autonomic Neurons ◽

Double Staining Method ◽

Adrenergic Fibers

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Identification of anti-golgi antibody by double-staining method with antibody to β-cop

Journal of Dermatological Science ◽

10.1016/0923-1811(93)91160-v ◽

1993 ◽

Vol 6 (1) ◽

pp. 77

Author(s):

Hong-Shang Hong ◽

Yu-Shung Lee ◽

Heng-Leong Chan ◽

Tseng-tong Kuo

Keyword(s):

Staining Method ◽

Double Staining ◽

Double Staining Method

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Immunocytochemical localization of the posterior lobe hormones and of their carrier proteins.

Journal of Histochemistry & Cytochemistry ◽

10.1177/28.5.6991593 ◽

1980 ◽

Vol 28 (5) ◽

pp. 469-471 ◽

Cited By ~ 3

Author(s):

F Vandesande ◽

K Dierickx ◽

N Goossens

Keyword(s):

Staining Method ◽

Posterior Lobe ◽

Serial Sections ◽

Double Staining ◽

Carrier Proteins ◽

Tissue Sections ◽

Tissue Antigens ◽

Staining Methods ◽

Double Staining Method ◽

The One

Serial sections of vertebrate hypothalami were stained with the immunocytochemical peroxidase-antiperoxidase method. In addition to the single staining method, our double staining method was used, which enabled us to visualize two tissue antigens in single tissue sections. In both staining methods, differentially adsorbed antineurohypophysial hormone sera, anti-somatostatin serum and anti-bovine neurophysin sera were used. The results confirm the one hormone, one neuron hypothesis.

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Results of a double staining method for DNA and RNA

The Science of Nature ◽

10.1007/bf00599725 ◽

1968 ◽

Vol 55 (10) ◽

pp. 496-497 ◽

Cited By ~ 2

Author(s):

Sibdas Ghosh ◽

Renate Lettr�

Keyword(s):

Staining Method ◽

Double Staining ◽

Dna And Rna ◽

Double Staining Method

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Combined beta-galactosidase and immunogold/silver staining for immunohistochemistry and DNA in situ hybridization.

Journal of Histochemistry & Cytochemistry ◽

10.1177/38.3.1689335 ◽

1990 ◽

Vol 38 (3) ◽

pp. 325-329 ◽

Cited By ~ 19

Author(s):

W van den Brink ◽

C van der Loos ◽

H Volkers ◽

R Lauwen ◽

F van den Berg ◽

...

Keyword(s):

In Situ Hybridization ◽

Silver Staining ◽

Staining Method ◽

Double Staining ◽

Reaction Products ◽

Silver Enhancement ◽

Silver Staining Method ◽

Double Staining Method ◽

Beta Galactosidase

A combination of beta-galactosidase enzyme and the immunogold/silver staining method was studied for evaluation of double-staining experiments. Applications are shown for immunohistochemical double staining using two monoclonal antibodies and for combined immunohistochemistry and DNA in situ hybridization in one tissue section. The following advantages for the present double-staining method were evaluated: superior sensitivity of the immunogold/silver staining method for at least one epitope, which also allows detection of biotinylated DNA probes. The structure of the indolyl precipitate after revelation of beta-galactosidase activity did not show a concealing effect during a sequential double-staining method, as compared with the visualization of peroxidase with diaminobenzidine. These factors, and the sharply contrasting colored reaction products of beta-galactosidase (blue-green) and the immunogold/silver staining method including silver enhancement (brown-black), allow clear distinction of mixed-stained cell constituents.

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Effect of Transmembrane Pressure on Geosmin Release from Cyanobacterial Cell During Microfiltration

Water Practice & Technology ◽

10.2166/wpt.2010.049 ◽

2010 ◽

Vol 5 (3) ◽

Cited By ~ 1

Author(s):

T. Matsushita ◽

Y. Kobayashi ◽

K. Nakamura ◽

Y. Matsui ◽

K. Ohno

Keyword(s):

Cell Viability ◽

Staining Method ◽

Transmembrane Pressure ◽

Cyanobacterial Cell ◽

Double Staining ◽

Filtration Time ◽

Viability Test ◽

A Cell ◽

Double Staining Method

The mechanisms of increase in geosmin concentration in MF permeate with filtration time were investigated when inflow to the MF contained cyanobacteria incorporating geosmin inside and outside their cells. Whereas the geosmin concentration in the MF permeate was almost the same as the extracellular geosmin concentration in the MF inflow at the beginning of filtration, it increased and exceeded the extracellular geosmin concentration in MF inflow after 2 h of filtration. A cell viability test using a double-staining method revealed that a portion of the intracellular geosmin, which was incorporated in the cyanobacterial cells and therefore retained on the MF membrane, was released from the cells. This occurred probably because increased transmembrane pressure (TMP) with filtration time forced the cells to compress. However, geosmin release was not explained simply by cell breakage: other possible factors were implied.

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