Secreted Antigens of Bacilli:

2021 ◽  
pp. 273-298
Author(s):  
E.P. Kiseleva ◽  
K.I. Mikhailopulo ◽  
D. Kołożyn-Krajewska ◽  
Galina I. Novik
Keyword(s):  
Secreted Antigens

scholarly journals Effect of Growth State on Transcription Levels of Genes Encoding Major Secreted Antigens of Mycobacterium tuberculosis in the Mouse Lung

2004 ◽  
Vol 72 (4) ◽  
pp. 2420-2424 ◽  
Author(s):  
Lanbo Shi ◽  
Robert North ◽  
Maria Laura Gennaro
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Adaptive Immunity ◽  
Mouse Lung ◽  
Mrna Levels ◽  
Growth State ◽  
Genes Encoding ◽  
Secreted Antigens

ABSTRACT Arrest of the multiplication of Mycobacterium tuberculosis caused by expression of adaptive immunity in mouse lung was accompanied by a 10- to 20-fold decrease in levels of mRNAs encoding the secreted Ag85 complex and 38-kDa lipoprotein. esat-6 mRNA levels were high throughout infection. The data imply that multiplying and nonreplicating tubercle bacilli have different antigen compositions.

scholarly journals Evaluation of Leishmania (Leishmania) infantum excreted-secreted antigens for detection of canine leishmaniasis

Acta Tropica ◽  
2016 ◽  
Vol 161 ◽  
pp. 41-43 ◽  
Author(s):  
Viviana Pinedo-Cancino ◽  
Márcia Dalastra Laurenti ◽  
Norival Kesper ◽  
Eufrosina Setsu Umezawa
Keyword(s):  
Leishmania Infantum ◽  
Canine Leishmaniasis ◽  
Secreted Antigens

scholarly journals Immunoblotting analyses using two-dimensional gel electrophoresis of Trypanosoma cruzi excreted-secreted antigens

2004 ◽  
Vol 37 (6) ◽  
pp. 454-459
Author(s):  
Adriano Gomes Silva ◽  
Elisangela Paula Silveira-Lacerda ◽  
Jair Pereira Cunha-Júnior ◽  
Maria Aparecida de Souza ◽  
Silvio Favoreto Junior
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Gel Electrophoresis ◽  
Complex Mixture ◽  
Chronic Phase ◽  
Disease Diagnosis ◽  
Basic Knowledge ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Secreted Antigens

Trypanosoma cruzi trypomastigotes excrete-secrete a complex mixture of antigenic molecules. This antigenic mixture denominated trypomastigote excreted-secreted antigens contains a 150-160 kDa band that shows excellent performance in Chagas' disease diagnosis by immunoblotting. The present study partially characterized by two-dimensional gel electrophoresis the immunoreactivity against the 150-160kDa protein using sera samples from chagasic patients in different phases of the disease. Trypomastigote excreted-secreted antigen preparations were subjected to high-resolution two-dimensional (2D) gel electrophoresis followed by immunoblotting with sera from chagasic and non-chagasic patients. The 150-160kDa protein presented four isoforms with isoelectric focusing ranging from 6.2 to 6.7. The four isoforms were recognized by IgM from acute phase and IgG from chronic phase sera of chagasic patients. The 150-160kDa isoform with IF of approximately 6.4 became the immunodominant spot with the progression of the disease. No cross-reactivity was observed with non-chagasic or patients infected with Leishmania sp. In this study we provide basic knowledge that supports the validation of trypomastigote excreted-secreted antigens for serological diagnosis of Chagas' disease.

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