Use of Embryonal Carcinoma Cells to Study Growth Factors During Early Mammalian Development

2021 ◽  
pp. 113-134
Author(s):  
Angie Rizzino
Keyword(s):  
Growth Factors ◽  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Mammalian Development ◽  
Early Mammalian Development

scholarly journals Lamins A and C appear during retinoic acid-induced differentiation of mouse embryonal carcinoma cells.

1987 ◽  
Vol 105 (3) ◽  
pp. 1099-1104 ◽  
Author(s):  
S Lebel ◽  
C Lampron ◽  
A Royal ◽  
Y Raymond
Keyword(s):  
Retinoic Acid ◽  
Embryonal Carcinoma ◽  
De Novo ◽  
Peptide Mapping ◽  
Nuclear Lamina ◽  
Polyclonal Antiserum ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Mammalian Development ◽  
Induced Differentiation

The lamin complement of nuclear matrix isolated from F9 embryonal carcinoma cells was studied during retinoic acid-induced differentiation in culture. Differentiation of the original cells into parietal endoderm-like cells was accompanied by the gradual appearance of lamins A and C while lamin B was present throughout all stages. Lamins were identified by their molecular masses, isoelectric points, recognition by a monoclonal antibody and a polyclonal antiserum, and by peptide mapping. The increase in the amounts of lamins A and C found in the matrix was due to de novo synthesis as no extranuclear pools of these lamins were detected in the undifferentiated cells. These results provide biochemical evidence that, as in amphibian embryogenesis, there are variations in nuclear lamina composition during mammalian development.

Identification and characterization of polypeptide growth factors secreted by murine embryonal carcinoma cells

1989 ◽  
Vol 133 (1) ◽  
pp. 272-283 ◽  
Author(s):  
Everardus J.J. van Zoelen ◽  
Theodora M.J. Ward-van Oostwaard ◽  
Rienk Nieuwland ◽  
Bart van der Burg ◽  
Adriana J.M. van den Eijnden-van Raaij ◽  
...  
Keyword(s):  
Growth Factors ◽  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Polypeptide Growth Factors ◽  
Identification And Characterization
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