Determination of Cations and Anions by Chromatographic and Electrophoretic Techniques

2004 ◽  
pp. 1891-1918 ◽  
Author(s):  
Christian Klampfl
Keyword(s):  
Electrophoretic Techniques

Applications of Electrophoretic Data in Avian Systematics

The Auk ◽  
1984 ◽  
Vol 101 (4) ◽  
pp. 717-729 ◽  
Author(s):  
Ronald H. Matson
Keyword(s):  
Genetic Distances ◽  
International Union ◽  
Regulation Of Expression ◽  
Egg White Proteins ◽  
Particulate Form ◽  
Electrophoretic Data ◽  
Electrophoretic Techniques ◽  
Methodological Problems ◽  
Systematic Relationships

AbstractThis paper is a survey of applications of electrophoretic techniques in ornithology, with an emphasis on post-1970 publications. The majority of electrophoretic studies of birds have been limited in a variety of ways. Many have dealt with "domesticated" species or have been limited to the examination of blood and/or egg-white proteins. Problems in comparing results from different studies have arisen because of: (1) dissimilar electrophoretic techniques; (2) varying numbers of taxa; (3) nonstandardized enzyme and locus nomenclature; and, especially, (4) different methods of data analysis. These methodological problems must be addressed in order to broaden the utility of electrophoretic data in avian systematics. I suggest that the enzyme names recognized by the International Union of Biochemistry be used exclusively and that a standardized locus nomenclature, comparable with that used in other vertebrate classes, be developed. The predominating use of allozyme characters can be supplemented by "isozyme characters" (e.g. different numbers of genes, heteropolymer assembly, and regulation of expression sensu Buth in press), which possibly could be applied to a determination of systematic relationships of higher-level taxonomic ranks. Allozyme and/or isozyme data should be retained in particulate form (i.e. not summarized as genetic distances). The use of outgroups to assign evolutionary direction is encouraged.

scholarly journals Dimorphic Mating-Type Chromosomes in the Fungus Microbotryum violaceum

Genetics ◽  
2002 ◽  
Vol 160 (2) ◽  
pp. 457-461 ◽  
Author(s):  
Michael E Hood
Keyword(s):  
Mating Type ◽  
Sex Chromosomes ◽  
Dna Sequences ◽  
Karyotype Analysis ◽  
Sequence Similarity ◽  
Large Degree ◽  
Microbotryum Violaceum ◽  
Mating Compatibility ◽  
Electrophoretic Techniques

Abstract Fungi often mate as haploids, and sex chromosomes (i.e., mating-type chromosomes) that are dimorphic for their size or overall DNA content have never been reported in this kingdom. Using electrophoretic techniques for karyotype analysis, a highly dimorphic chromosome pair that determines mating compatibility is shown to occur in populations of the fungus Microbotryum violaceum. This substantiates the evolution of such dimorphism as a general feature associated with haploid determination of mating compatibility, which previously had been known only in haplodioecious plants (mosses and liverworts). Size-dimorphic sex chromosomes are present in a lineage of M. violaceum native to Europe, as well as a lineage native to North America. However, they are very different in size between these lineages, indicating either independent evolution of the dimorphism or a large degree of divergence since their isolation. Several DNA sequences that show sequence similarity to transposons were isolated from these sex chromosomes.

Determination of basic components in white wines by HPLC, FT-IR spectroscopy, and electrophoretic techniques

1992 ◽  
Vol 5 (3) ◽  
pp. 236-245 ◽  
Author(s):  
S. Gorinstein ◽  
R. Moshe ◽  
J. Deutsch ◽  
F.H. Wolfed ◽  
K. Tilis ◽  
...  
Keyword(s):  
Ir Spectroscopy ◽  
White Wines ◽  
Ft Ir ◽  
Electrophoretic Techniques ◽  
Ft Ir Spectroscopy

Measurement of low-density-lipoprotein cholesterol in serum: a status report

1992 ◽  
Vol 38 (1) ◽  
pp. 150-160 ◽  
Author(s):  
Nader Rifai ◽  
G Russell Warnick ◽  
Judith R McNamara ◽  
John D Belcher ◽  
Gregory F Grinstead ◽  
...  
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Status Report ◽  
Low Density ◽  
Advantages And Disadvantages ◽  
Clinical Laboratories ◽  
Friedewald Equation ◽  
Electrophoretic Techniques ◽  
Classification And Treatment

Abstract Current recommendations of the Adult Treatment Panel and the Children and Adolescents Treatment Panel of the National Cholesterol Education Program make the concentration of low-density lipoproteins cholesterol (LDL-C) in serum the basis for the classification and treatment of hypercholesterolemia. Numerous methodologies for the determination of serum LDL-C concentrations, in research and clinical laboratories, have been described. Here, we review the principles, performance, and limitations of major current methodologies for determining LDL-C concentrations. These methods include sequential and density-gradient ultracentrifugation, chromatographic and electrophoretic techniques, and precipitation methods. In addition, the advantages and disadvantages of estimating LDL-C concentration by the Friedewald equation, the most commonly used approach in clinical laboratories, are addressed.

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