Monitoring Cyclic Nucleotides Using Genetically Encoded Fluorescent Reporters

2015 ◽  
pp. 98-115
Keyword(s):  
Cyclic Nucleotides ◽  
Fluorescent Reporters

Arabidopsis 4-COUMAROYL-COA LIGASE 8 contributes to the biosynthesis of the benzenoid ring of coenzyme Q in peroxisomes

2019 ◽  
Vol 476 (22) ◽  
pp. 3521-3532
Author(s):  
Eric Soubeyrand ◽  
Megan Kelly ◽  
Shea A. Keene ◽  
Ann C. Bernert ◽  
Scott Latimer ◽  
...  
Keyword(s):  
Oxidative Metabolism ◽  
Time Course ◽  
Reverse Genetics ◽  
De Novo ◽  
Coenzyme Q ◽  
Control Level ◽  
Wild Type ◽  
Fluorescent Reporters ◽  
Coa Ligase ◽  
Peroxisomal Targeting

Plants have evolved the ability to derive the benzenoid moiety of the respiratory cofactor and antioxidant, ubiquinone (coenzyme Q), either from the β-oxidative metabolism of p-coumarate or from the peroxidative cleavage of kaempferol. Here, isotopic feeding assays, gene co-expression analysis and reverse genetics identified Arabidopsis 4-COUMARATE-COA LIGASE 8 (4-CL8; At5g38120) as a contributor to the β-oxidation of p-coumarate for ubiquinone biosynthesis. The enzyme is part of the same clade (V) of acyl-activating enzymes than At4g19010, a p-coumarate CoA ligase known to play a central role in the conversion of p-coumarate into 4-hydroxybenzoate. A 4-cl8 T-DNA knockout displayed a 20% decrease in ubiquinone content compared with wild-type plants, while 4-CL8 overexpression boosted ubiquinone content up to 150% of the control level. Similarly, the isotopic enrichment of ubiquinone's ring was decreased by 28% in the 4-cl8 knockout as compared with wild-type controls when Phe-[Ring-13C6] was fed to the plants. This metabolic blockage could be bypassed via the exogenous supply of 4-hydroxybenzoate, the product of p-coumarate β-oxidation. Arabidopsis 4-CL8 displays a canonical peroxisomal targeting sequence type 1, and confocal microscopy experiments using fused fluorescent reporters demonstrated that this enzyme is imported into peroxisomes. Time course feeding assays using Phe-[Ring-13C6] in a series of Arabidopsis single and double knockouts blocked in the β-oxidative metabolism of p-coumarate (4-cl8; at4g19010; at4g19010 × 4-cl8), flavonol biosynthesis (flavanone-3-hydroxylase), or both (at4g19010 × flavanone-3-hydroxylase) indicated that continuous high light treatments (500 µE m−2 s−1; 24 h) markedly stimulated the de novo biosynthesis of ubiquinone independently of kaempferol catabolism.

scholarly journals The Regulation of Lipogenesis by Cyclic Nucleotides in Intact Hepatocytes Prepared by a Simplified Technique

1974 ◽  
Vol 249 (4) ◽  
pp. 1286-1294
Author(s):  
David M. Capuzzi ◽  
Vicki Rothman ◽  
Simeon Margolis
Keyword(s):  
Cyclic Nucleotides

scholarly journals Extracellular Modulation of the Silkmoth Sex Pheromone Receptor Activity by Cyclic Nucleotides

PLoS ONE ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. e63774 ◽  
Author(s):  
Tatsuro Nakagawa ◽  
Kazushige Touhara
Keyword(s):  
Sex Pheromone ◽  
Cyclic Nucleotides ◽  
Receptor Activity ◽  
Pheromone Receptor

scholarly journals Patch Cramming Reveals the Mechanism of Long-Term Suppression of Cyclic Nucleotides in Intact Neurons

2002 ◽  
Vol 22 (20) ◽  
pp. 8819-8826 ◽  
Author(s):  
Bhavya Trivedi ◽  
Richard H. Kramer
Keyword(s):  
Cyclic Nucleotides

scholarly journals Radioimmunoassay for Cyclic Nucleotides

1972 ◽  
Vol 247 (4) ◽  
pp. 1114-1120 ◽  
Author(s):  
Alton L. Steiner ◽  
Anthony S. Pagliara ◽  
Lewis R. Chase ◽  
David M. Kipnis
Keyword(s):  
Cyclic Nucleotides

Effects of hypokinesia on cyclic nucleotides and hormonal regulation of calcium metabolism in rats

2002 ◽  
Vol 79 (4) ◽  
Author(s):  
J. B.L. Chek ◽  
A. I. Laniko
Keyword(s):  
Cyclic Nucleotides ◽  
Hormonal Regulation ◽  
Calcium Metabolism

Role of cyclic nucleotides in the mechanism of action of enkephalins

1982 ◽  
Vol 93 (3) ◽  
pp. 265-267
Author(s):  
M. Ya. Maizelis ◽  
A. L. Zabludovskii ◽  
S. N. Shikhov
Keyword(s):  
Mechanism Of Action ◽  
Cyclic Nucleotides
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