scholarly journals LIM Homeodomain Transcription Factor Isl-1 Enhances Follicle Stimulating Hormone-β and Luteinizing Hormone-β Gene Expression and Mediates the Activation of Leptin on Gonadotropin Synthesis

Endocrinology ◽  
2010 ◽  
Vol 151 (10) ◽  
pp. 4787-4800 ◽  
Author(s):  
Yingjie Wu ◽  
Haoshu Luo ◽  
Jiali Liu ◽  
Duo Kang ◽  
Alan S. McNeilly ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Homeodomain Transcription Factor ◽  
Lim Homeodomain ◽  
Stimulating Hormone

scholarly journals LbetaT2 gonadotroph cells secrete follicle stimulating hormone (FSH) in response to active A

1999 ◽  
Vol 162 (3) ◽  
pp. R1-R5 ◽  
Author(s):  
KE Graham ◽  
KD Nusser ◽  
MJ Low
Keyword(s):  
Gene Expression ◽  
Luteinizing Hormone ◽  
Cell Line ◽  
Follicle Stimulating Hormone ◽  
Activin A ◽  
Gonadotropin Releasing Hormone ◽  
Ribonuclease Protection Assay ◽  
Protection Assay ◽  
Ribonuclease Protection ◽  
Stimulating Hormone

Secretion of luteinizing hormone in response to gonadotropin releasing hormone (GnRH) has been described in the recently developed LbetaT2 gonadotroph cell line. We evaluated the expression of follicle stimulating hormone (FSH)beta mRNA and secretion of FSH from LbetaT2 cells in response to GnRH and activin A. LbetaT2 cells were treated with activin A in doses from 0 to 50 ng/ml, with or without a daily 10 nM GnRH pulse, or with GnRH alone. FSH secretion was stimulated over 6-fold by concomitant GnRH and activin A in a dose-responsive fashion at 72 h of treatment. FSHbeta mRNA was detectable by ribonuclease protection assay only in cells treated with activin A with or without GnRH. The demonstration of FSHbeta gene expression in LbetaT2 cells further validates these cells as mature, differentiated gonadotrophs and as an important tool for the study of gonadotroph physiology.

Pituitary transcription factor Prop-1 stimulates porcine follicle-stimulating hormone β subunit gene expression

2004 ◽  
Vol 324 (2) ◽  
pp. 946-952 ◽  
Author(s):  
Satoko Aikawa ◽  
Takako Kato ◽  
Takao Susa ◽  
Kyoko Tomizawa ◽  
Satoshi Ogawa ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Follicle Stimulating Hormone ◽  
Β Subunit ◽  
Subunit Gene ◽  
Stimulating Hormone
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