scholarly journals LbetaT2 gonadotroph cells secrete follicle stimulating hormone (FSH) in response to active A

1999 ◽  
Vol 162 (3) ◽  
pp. R1-R5 ◽  
Author(s):  
KE Graham ◽  
KD Nusser ◽  
MJ Low
Keyword(s):  
Gene Expression ◽  
Luteinizing Hormone ◽  
Cell Line ◽  
Follicle Stimulating Hormone ◽  
Activin A ◽  
Gonadotropin Releasing Hormone ◽  
Ribonuclease Protection Assay ◽  
Protection Assay ◽  
Ribonuclease Protection ◽  
Stimulating Hormone

Secretion of luteinizing hormone in response to gonadotropin releasing hormone (GnRH) has been described in the recently developed LbetaT2 gonadotroph cell line. We evaluated the expression of follicle stimulating hormone (FSH)beta mRNA and secretion of FSH from LbetaT2 cells in response to GnRH and activin A. LbetaT2 cells were treated with activin A in doses from 0 to 50 ng/ml, with or without a daily 10 nM GnRH pulse, or with GnRH alone. FSH secretion was stimulated over 6-fold by concomitant GnRH and activin A in a dose-responsive fashion at 72 h of treatment. FSHbeta mRNA was detectable by ribonuclease protection assay only in cells treated with activin A with or without GnRH. The demonstration of FSHbeta gene expression in LbetaT2 cells further validates these cells as mature, differentiated gonadotrophs and as an important tool for the study of gonadotroph physiology.

scholarly journals Annual Change of Reproductive Hormones in Female Angora Goats

2017 ◽  
Vol 5 (4) ◽  
pp. 343
Author(s):  
Erkan Pehlivan ◽  
Hüseyin Polat ◽  
Gürsel Dellal
Keyword(s):  
Luteinizing Hormone ◽  
Repeated Measures ◽  
Follicle Stimulating Hormone ◽  
Reproductive Hormones ◽  
Gonadotropin Releasing Hormone ◽  
Blood Samples ◽  
Releasing Hormone ◽  
Angora Goats ◽  
Turkish State ◽  
Stimulating Hormone

In this research, annual changes of melatonin, gonadotropin-releasing hormone, follicle stimulating hormone, luteinizing hormone, estrogen, testosterone and progesterone were studied on 6 heads of 1.5 years old female Angora goat. To determine hormones concentrations, blood samples were taken from jugular vein of each goat in every month for a year. The blood samples were centrifuged at 4000xg for 5 min. and serum was stored at -20°C until analyses time. Hormones analyses in the serum were performed by enzyme immunoassay (EIA) method. Monthly climatic values and photoperiod were obtained from the Turkish State Meteorological Service and temperature-humidity index was calculated with climatic values. In the study, in order to determine any possible differences in the observed hormones concentrations with respect to months, repeated measures ANOVA analysis was performed. As a result of statistical analysis, there were no significant differences among the months for gonadotropin-releasing hormone, follicle stimulating hormone and testosterone concentration, while significant differences were found among the months for melatonin, luteinizing hormone and progesterone, and estrogen concentration in female Angora goats. According the results of this study, could be concluded that the releases of reproductive hormones examined in female Angora goats was seasonally dependent.

Luteinizing hormone and follicle-stimulating hormone responses to intransal gonadotropin-releasing hormone *

1986 ◽  
Vol 45 (6) ◽  
pp. 794-799 ◽  
Author(s):  
Jacob Rajfer ◽  
David J. Handelsman ◽  
Ronald S. Swerdloff ◽  
John H. Farrer ◽  
Suresh C. Sikka
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Gonadotropin Releasing Hormone ◽  
Releasing Hormone ◽  
Hormone Responses ◽  
Stimulating Hormone
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