The Use of a Photoaffinity Ligand to Compare Androgen-Binding Protein (ABP) Present in Rat Sertoli Cell Culture Media with ABP Present in Epididymal Cytosol*

Endocrinology ◽  
1981 ◽  
Vol 108 (3) ◽  
pp. 786-794 ◽  
Author(s):  
WARREN N. SCHMIDT ◽  
CLINTON A. TAYLOR ◽  
BENJAMIN J. DANZO
Keyword(s):  
Cell Culture ◽  
Sertoli Cell ◽  
Culture Media ◽  
Binding Protein ◽  
Cell Culture Media ◽  
Androgen Binding Protein ◽  
Androgen Binding ◽  
Sertoli Cell Culture ◽  
Photoaffinity Ligand

ANALYSIS OF ANDROGEN-BINDING PROTEIN IN MEDIA FROM SERTOLI CELL INCUBATIONS AND CYTOSOLS FROM RAT TESTES

1977 ◽  
Vol 73 (3) ◽  
pp. 511-517 ◽  
Author(s):  
F. F. G. ROMMERTS ◽  
B. Ch. KRÜGER-SEWNARAIN ◽  
J. A. GROOTEGOED ◽  
H. J. VAN DER MOLEN
Keyword(s):  
Sertoli Cell ◽  
Binding Proteins ◽  
Culture Media ◽  
Binding Protein ◽  
Equilibrium Dialysis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Testicular Tissue ◽  
Androgen Binding Protein ◽  
Steroid Binding Proteins ◽  
Androgen Binding

SUMMARY Binding ability of androgen-binding protein (ABP) in concentrated media from Sertoli cell cultures and in various preparations of cytosols from rat testicular tissue have been estimated using dialysis and polyacrylamide gel electrophoresis (PAGE). Equilibrium dialysis in the presence of 1 nm-17β-hydroxy-5α-androstan-3-one appears to be the most convenient and reliable method for estimation of ABP if no other binding proteins are present. PAGE can be applied for estimation of ABP in samples which also contain other steroid-binding proteins. Estimated binding activities in culture media measured by PAGE and dialysis were essentially similar. Binding of androgens to ABP estimated with PAGE in cytosols prepared after sonication of testicular tissue was much lower than in cytosols obtained after homogenization using a Potter–Elvehjem homogenizer. When ABP was added to cytosols prepared after sonication or homogenization, approximately 50 and 90% respectively of the original binding of androgens to ABP was recovered.

Testicular Androgen Binding Protein (ABP) — A Parameter of Sertoli Cell Secretory Function

1975 ◽  
pp. 323-336 ◽  
Author(s):  
V. Hansson ◽  
S. C. Weddington ◽  
O. Naess ◽  
A. Attramadal ◽  
F. S. French ◽  
...  
Keyword(s):  
Sertoli Cell ◽  
Binding Protein ◽  
Secretory Function ◽  
Androgen Binding Protein ◽  
Androgen Binding

Sertoli cell-specific expression of rat androgen-binding protein in transgenic mice: effects on somatic cell lineages

1997 ◽  
Vol 132 (1-2) ◽  
pp. 127-136 ◽  
Author(s):  
Cristina Esteban ◽  
Anne Gérard ◽  
Sara Larrib ◽  
Núria Torán ◽  
Hubert Gérard ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Somatic Cell ◽  
Sertoli Cell ◽  
Binding Protein ◽  
Specific Expression ◽  
Cell Lineages ◽  
Androgen Binding Protein ◽  
Androgen Binding

Sertoli cell origin of testicular androgen-binding protein (ABP)

1975 ◽  
Vol 2 (5) ◽  
pp. 339-350 ◽  
Author(s):  
L. Hagenäs ◽  
E.M. Ritzén ◽  
L. Plöen ◽  
V. Hansson ◽  
F.S. French ◽  
...  
Keyword(s):  
Sertoli Cell ◽  
Binding Protein ◽  
Androgen Binding Protein ◽  
Androgen Binding

Establishment of a Mouse Sertoli Cell Line Producing Rat Androgen-binding Protein (ABP)

Steroids ◽  
1998 ◽  
Vol 63 (5-6) ◽  
pp. 285-287 ◽  
Author(s):  
Angélique Ducray ◽  
Michèle Bloquel ◽  
Ketsia Hess ◽  
Geoffrey L. Hammond ◽  
Hubert Gérard ◽  
...  
Keyword(s):  
Cell Line ◽  
Sertoli Cell ◽  
Binding Protein ◽  
Androgen Binding Protein ◽  
Androgen Binding ◽  
Sertoli Cell Line
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