A New Tissue-Slicing Method for the Study of Function and Position of Somatotrophs Contained within the Male Rat Pituitary Gland*

Endocrinology ◽  
1990 ◽  
Vol 127 (4) ◽  
pp. 1877-1886 ◽  
Author(s):  
FRANK M. PEREZ ◽  
W. C. HYMER
Keyword(s):  
Pituitary Gland ◽  
Male Rat ◽  
Rat Pituitary ◽  
Slicing Method

Effects of Morphine and Naloxone on Prolactin and Growth Hormone Gene Expression in the Male Rat Pituitary Gland

1993 ◽  
Vol 5 (5) ◽  
pp. 553-556 ◽  
Author(s):  
Pablo-Manuel Dobado-Berrios ◽  
Songuyn Li ◽  
Ester Garcia Yebenes ◽  
Georges Pelletier
Keyword(s):  
Gene Expression ◽  
Growth Hormone ◽  
Pituitary Gland ◽  
Male Rat ◽  
Growth Hormone Gene ◽  
Rat Pituitary

Septate-like junctions in the normal male rat pituitary gland

1994 ◽  
Vol 26 (6) ◽  
pp. 913-916 ◽  
Author(s):  
Chieko Kurono ◽  
Masumi Nozaki ◽  
Hiroshi Ohguchi ◽  
Tsuyoshi Soji ◽  
Damon C. Herbert
Keyword(s):  
Pituitary Gland ◽  
Male Rat ◽  
Normal Male ◽  
Rat Pituitary

2-HYDROXYOESTRADIOL INHIBITS PROLACTIN RELEASE FROM THE SUPERFUSED RAT PITUITARY GLAND

1981 ◽  
Vol 90 (3) ◽  
pp. 315-322 ◽  
Author(s):  
ELIZABETH A. LINTON ◽  
NICKI WHITE ◽  
OFELIA LIRA DE TINEO ◽  
S. L. JEFFCOATE
Keyword(s):  
Pituitary Gland ◽  
Prolactin Secretion ◽  
Male Rat ◽  
Prolactin Release ◽  
Rat Pituitary ◽  
Lh Release

The effects of 2-hydroxyoestradiol (2OH-OE2), dopamine, oestradiol-17β and 2OH-OE2 plus dopamine on prolactin and LH release from the male rat pituitary gland were examined in vitro. 2-Hydroxyoestradiol reduced prolactin secretion by 51% at 10−10 mol/l and by 34% at 10−7 mol/l, while oestradiol-17β had no effect at these doses. Dopamine alone (5 × 10−7 mol/l) decreased prolactin released by 58%, 2OH-OE2 plus dopamine produced a similar inhibition of 60%. No significant effect on LH release was observed throughout.

ONSET OF OESTROGEN-INDUCED PROLACTIN SECRETION AND DNA SYNTHESIS BY THE RAT PITUITARY GLAND

1977 ◽  
Vol 72 (1) ◽  
pp. 35-39 ◽  
Author(s):  
JOAN JACOBI ◽  
H. M. LLOYD ◽  
J. D. MEARES
Keyword(s):  
Dna Synthesis ◽  
Pituitary Gland ◽  
Prolactin Secretion ◽  
Male Rat ◽  
Serum Prolactin ◽  
Rat Pituitary ◽  
The Times ◽  
Pituitary Prolactin

SUMMARY The times of onset of oestrogen-induced prolactin secretion and DNA synthesis were studied in the pituitary gland of the male rat. At intervals from 3 to 96 h after injection of 10 mg diethylstilboestrol dipropionate, serum and pituitary prolactin concentrations were measured by radioimmunoassay and pituitary DNA synthesis by incorporation of [3H]thymidine in vitro. Serum prolactin was raised significantly from 6 h onwards and DNA synthesis was increased from 30 h onwards. Pituitary prolactin concentration began to increase at 30 h. Significant correlations were obtained between serum prolactin and DNA synthesis from 24 to 72 h but not during the period of prolactin secretion from 6 to 24 h.

ANTI-OESTROGENIC EFFECTS OF NAFOXIDINE ON THE SYNTHESIS OF DNA BY THE RAT PITUITARY GLAND

1978 ◽  
Vol 76 (3) ◽  
pp. 555-556 ◽  
Author(s):  
J. M. JACOBI ◽  
H. M. LLOYD
Keyword(s):  
Pituitary Gland ◽  
Male Rat ◽  
Serum Prolactin ◽  
Free Access ◽  
Sprague Dawley ◽  
Target Organs ◽  
Sprague Dawley Rats ◽  
Rat Pituitary ◽  
Pelleted Diet ◽  
Principal Actions

Department of Medicine, University of Queensland, Clinical Sciences Building, Royal Brisbane Hospital, Queensland 4029, Australia Received 25 October 1977 One of the principal actions of oestrogens on their target organs is stimulation of the synthesis of DNA and cell division. In the rat pituitary gland, anti-oestrogens inhibit the oestrogen-induced increase in weight (Schreiber, Přibyl & Roháčová, 1972) and prevent the rise in the level of serum prolactin induced by oestradiol-17β (Jordan, Koerner & Robinson, 1975). The effects of anti-oestrogens on the synthesis of DNA by the pituitary gland have not been described. The experiments reported here show that the anti-oestrogen nafoxidine completely inhibits the synthesis of DNA and affects the secretion of growth hormone (GH) and prolactin in the male rat. Male Sprague–Dawley rats, 100 days old, were allowed free access to a pelleted diet and water and were maintained in a controlled environment (12 h light, 12 h darkness).

Evidence for direct action of calcitonin in the rat pituitary gland

1989 ◽  
Vol 120 (5) ◽  
pp. 682-688 ◽  
Author(s):  
G. Morel ◽  
J.-G. Chabot ◽  
A. Enjalbert ◽  
M. Priam ◽  
P. M. Dubois
Keyword(s):  
Pituitary Gland ◽  
Anterior Pituitary ◽  
Direct Action ◽  
Secretory Granules ◽  
In Vitro Study ◽  
Male Rat ◽  
Pituitary Cells ◽  
Cytoplasmic Matrix ◽  
Rat Pituitary

Abstract. Classic concepts of calcitonin (CT) function have focused on the effects of CT on calcium homeostasis. More recently CT actions on brain and pituitary have been investigated. In order to evaluate the effects of CT on the anterior pituitary gland we studied the action(s) of CT in vitro and visualized endogenous CT in adult male rat pituitary gland by immunocytochemistry on ultrathin sections obtained by cryoultramicomy. In vitro study using dispersed anterior pituitary cells indicated that CT stimulated the secretion of PRL, whereas the secretion of GH, TSH and LH was not affected. CT-like immunoreactivity was observed in lactotropes only. The other pituitary cell types were not immunoreactive. In lactotropes, immunostaining was observed in the cytoplasm and in the nucleus. In the cytoplasm, CT-like immunoreactivity was visuzalized in the cytoplasmic matrix and in the secretory granules. In the nucleus, immunostaining was distributed primarly in the euchromatin, in the vincinity of heterochromatin region. CT-like immunoreactivity was also observed at the plasma membrane but was only scarce. No reaction product was found when anti-CT serum pre-incubated with CT was used. In conclusion, these results bring evidence for a direct action of CT on lactotrope regulation in vitro as well as in intact animals.

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