Effects of Bovine Growth Hormone Preparations, Fragments of Growth Hormone and Pituitary Anti-insulin Peptide on Lipolysis and Glucose Metabolism of Isolated Fat Cells and Adipose Tissue*

Endocrinology ◽  
1974 ◽  
Vol 95 (1) ◽  
pp. 206-212 ◽  
Author(s):  
PAUL B. WIESER ◽  
JAMES A. MALGIERI ◽  
WALTER F. WARD ◽  
RICHARD H. POINTER ◽  
JOHN N. FAIN
Keyword(s):  
Growth Hormone ◽  
Adipose Tissue ◽  
Glucose Metabolism ◽  
Bovine Growth Hormone ◽  
Fat Cells ◽  
Isolated Fat Cells

LIPOLYSIS IN CHICKEN ADIPOSE TISSUE IN VITRO

1969 ◽  
Vol 43 (2) ◽  
pp. 285-294 ◽  
Author(s):  
D. R. LANGSLOW ◽  
C. N. HALES
Keyword(s):  
Growth Hormone ◽  
Adipose Tissue ◽  
Sodium Succinate ◽  
Fat Cells ◽  
Propranolol Hydrochloride ◽  
Isolated Fat Cells ◽  
Adipose Tissue In Vitro ◽  
High Concentrations ◽  
Long Acting

SUMMARY The effects on lipolysis of various compounds have been studied in intact chicken adipose tissue and in isolated fat cells prepared from chicken adipose tissue. Glucagon stimulated lipolysis at concentrations down to 1 ng./ml. in intact pieces and 0·1 ng./ml. in isolated fat cells. The effect was enhanced by high concentrations of insulin. No anti-lipolytic effect of insulin was observed. Adrenaline, noradrenaline, porcine corticotrophin (ACTH) and long-acting ACTH were lipolytic but the effects were small and high concentrations were required. The adrenaline effect was blocked by propranolol hydrochloride. Dibutyryl 3′,5′-(cyclic)-AMP and theophylline stimulated lipolysis as did a combination of crude chicken growth hormone and hydrocortisone sodium succinate. It was concluded that the pattern of response of chicken adipose tissue was markedly different from that of the rat.

Reduced glucose incorporation to triglycerides following chronic exposure of human fat cells to growth hormone

1980 ◽  
Vol 95 (1) ◽  
pp. 129-133 ◽  
Author(s):  
Gudrun Nyberg ◽  
Stig Boström ◽  
Rolf Johansson ◽  
Ulf Smith
Keyword(s):  
Growth Hormone ◽  
Adipose Tissue ◽  
Glucose Metabolism ◽  
Human Growth ◽  
Human Adipose Tissue ◽  
Culture Technique ◽  
Fat Cells ◽  
Glucose Incorporation ◽  
Total Glucose

Abstract. Using the tissue culture technique we have recently demonstrated that long-term exposure of human adipose tissue to human growth hormone (GH) in vitro leads to an impairment in glucose incorporation into triglycerides. This effect was further studied in the present investigation. Biopsies of human adipose tissue which had been cultured for one week with or without GH were studied in subsequent short-term incubations where the conversion of glucose to CO2 and to total lipids was determined. The formation of CO2 was not changed by previous exposure of the biopsies to GH whereas the incorporation of glucose into triglycerides was reduced by about one third. Total glucose metabolism, as determined from the sum of the two pathways, was significantly reduced. The activities of three glycolytic enzymes were determined in biopsies of human adipose tissue which had been cultured with or without GH for one week. The activity of phosphofructokinase was reduced, while the hexokinase and the glucose-6-phosphate dehydrogenase activities were unchanged. The diminished activity of phosphofructokinase, the enzyme considered to be rate-limiting for glycolysis in human fat cells, may be responsible for the decreased rate of glucose metabolism found.

Effect of dihydroergotamine and growth hormone on lipolysis in isolated fat cells of the rat

1970 ◽  
Vol 48 (5) ◽  
pp. 324-326 ◽  
Author(s):  
N. Hotta ◽  
O. V. Sirek ◽  
A. Sirek
Keyword(s):  
Growth Hormone ◽  
Bovine Growth Hormone ◽  
Fat Cells ◽  
Isolated Fat Cells ◽  
Cell Preparation ◽  
Fat Cell ◽  
Lipolytic Effect ◽  
Mechanism Of Interaction

Dihydroergotamine (DHE) is lipolytic in the isolated fat cell preparation of Rodbell. Bovine growth hormone (GH) alone or in combination with dexamethasone causes lipolysis that is barely measurable. The combination of GH and DHE is equally ineffective. Incubation of fat cells with all three agents produces massive lipolysis that is by far greater than the sum of effects of individual components. The augmented lipolytic effect is suppressible with puromycin. The exact mechanism of interaction between GH, dexamethasone, and DHE has to be elucidated.

Native and Recombinant Bovine Growth Hormone Antagonize Insulin Action in Cultured Bovine Adipose Tissue*

Endocrinology ◽  
1987 ◽  
Vol 121 (2) ◽  
pp. 699-703 ◽  
Author(s):  
TERRY D. ETHERTON ◽  
CHRISTINA M. EVOCK ◽  
RONALD S. KENSINGER
Keyword(s):  
Growth Hormone ◽  
Adipose Tissue ◽  
Insulin Action ◽  
Bovine Growth Hormone

scholarly journals Effects of cytochalasin B, colchicine and vincristine on the metabolism of isolated fat-cells

1974 ◽  
Vol 140 (2) ◽  
pp. 185-192 ◽  
Author(s):  
Ernest G. Loten ◽  
Bernard Jeanrenaud
Keyword(s):  
Glucose Metabolism ◽  
Transport System ◽  
Cytochalasin B ◽  
Fat Cells ◽  
Isolated Fat Cells ◽  
Prolonged Incubation ◽  
Glucose Transport System ◽  
End Products ◽  
Release Of Glycerol ◽  
Cytochalasin A

1. Colchicine and vincristine only slightly inhibit the metabolism of glucose to CO2 and lipids by isolated fat-cells. 2. Prolonged incubation with these agents causes no further inhibition. 3. Cytochalasin B, however, inhibits glucose metabolism to both CO2 and lipids in fat-cells. 4. However, at a concentration that causes a strong inhibition of glucose metabolism cytochalasin B is without effect on the metabolism of pyruvate, lactate or arginine to these end products. The uptake of labelled α-aminoisobutyrate is likewise not modified. Similarly it does not affect release of glycerol or free fatty acid, or the actions of adrenaline, insulin or caffeine on these parameters. At 10μg/ml it slightly lowers ATP concentrations, an effect that does not occur at 2μg/ml. 5. The transport of fructose into adipocytes by a specific fructose-transport system is also not affected by the agent, but the uptake of 2-deoxyglucose is strongly inhibited. It is concluded that cytochalasin B may specifically inhibit the glucose-transport system of isolated fat-cells. 6. Cytochalasin A has a much weaker action than cytochalasin B on glucose metabolism.

scholarly journals Effect of nutrition on subcellular localization of rat fat-cell lipoprotein lipase

1978 ◽  
Vol 172 (2) ◽  
pp. 239-245 ◽  
Author(s):  
A Vanhove ◽  
C Wolf ◽  
M Breton ◽  
M C Glangeaud
Keyword(s):  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Plasma Membranes ◽  
Total Activity ◽  
Normal Diet ◽  
Epididymal Adipose Tissue ◽  
Fat Cells ◽  
Isolated Fat Cells ◽  
Fat Cell ◽  
Intact Tissue

This study supports the possibility for multiple subcellular forms of lipoprotein lipase. 1. The total activity of lipoprotein lipase per g of intact epididymal adipose tissue from fed rats is much higher than that from starved rats. 2. The isolated fat-cells of fed and of starved rats have lipoprotein lipase of almost the same activity per g of fat-pads. The isolated fat-cells of starved rats have a much higher proportion of total activity per g of the intact tissue than do those of fed rats. 3. Under the conditions of homogenization used, only a small proportion of the total activity per g of intact tissue from fed rats was associated with the fat layer which floated to the top of the homogenate during low-speed centrifugation. The different proportions of the specific enzyme activity found in each subcellular fraction are described. 4. Lipoprotein lipase from plasma membranes and microsomal fractions from starved and fed rats was purified by affinity chromatography. 5. The total activity of microsomal lipoprotein lipase per g of intact adipose tissue is enhanced by a normal diet. 6. In intact epididymal adipose tissue from fed rats, the activity per g of tissue of lipoprotein lipase of plasma membranes is much higher than that in the same fraction from starved rats. By contrast, the activities per g of tissue in plasma membranes obtained from starved or from fed rats by collagenase treatment were similar.

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