LC-MSMS Method Development for Steroids Panel Analysis in Human Serum.

2010 ◽  
pp. P3-41-P3-41
Author(s):  
Hua-fen Liu ◽  
John McFarlane ◽  
Renee Huang ◽  
Adam Latawiec ◽  
Lisa Sapp
Keyword(s):  
Human Serum ◽  
Method Development ◽  
Panel Analysis

scholarly journals Method development of immunoglobulin G purification from micro-volumes of human serum for untargeted and targeted proteomics-based antibody repertoire studies

2019 ◽  
Vol 27 (2) ◽  
pp. 475-482
Author(s):  
Yu-Ting Chang ◽  
Ming-Chu Chang ◽  
Yun-Jung Tsai ◽  
Christine Ferng ◽  
Hsi-Chang Shih ◽  
...  
Keyword(s):  
Human Serum ◽  
Immunoglobulin G ◽  
Method Development ◽  
Antibody Repertoire ◽  
Targeted Proteomics

Method Development and Validation for Ultra-High-Pressure LC/MS/MS Determination of Hop Prenylflavonoids in Human Serum

2012 ◽  
Vol 95 (6) ◽  
pp. 1744-1749 ◽  
Author(s):  
Yang Yuan ◽  
Xi Qiu ◽  
Dejan Nikolic ◽  
Jeffrey H Dahl ◽  
Richard B van Breemen
Keyword(s):  
High Pressure ◽  
Human Serum ◽  
Method Development ◽  
Hot Flashes ◽  
Cancer Chemoprevention ◽  
Gradient Elution ◽  
Negative Ion ◽  
Selected Reaction Monitoring ◽  
Ultra High Pressure

Abstract Hops (Humulus lupulus L.) are used in the brewing of beer, and hop extracts containing prenylated compounds, such as xanthohumol (XN) and 8-prenylnaringenin (8-PN), are under investigation as dietary supplements for cancer chemoprevention and the management of hot flashes in menopausal women. To facilitate clinical studies of hop safety and efficacy, a selective, sensitive, and fast ultra-high-pressure LC (UHPLC) tandem MS method was developed and validated for the simultaneous determination of the hop prenylflavonoids XN, isoxanthohumol (IX), 6-prenylnaringenin (6-PN), and 8-PN in human serum. The analytical method requires 300 μL of human serum, which is processed using liquid–liquid extraction. UHPLC separation was carried out in 2.5 min with gradient elution using an RP C18 column containing 1.6 μm particle size packing material. Prenylflavonoids were measured using negative ion electrospray MS with collision-induced dissociation and selected reaction monitoring. The method was validated and showed good accuracy and precision with an LOQ of 0.50 ng/mL for XN (1.4 nM) and 1.0 ng/mL for 6-PN, 8-PN (2.94 nM), and IX (2.82 nM) in serum.

Novel Method Development for Extraction and Analysis of Pesticide Residues in Human Serum Samples

2021 ◽  
Vol 18 (4) ◽  
pp. 79-86
Author(s):  
Divya Kottadiyil ◽  
Shital Deore ◽  
P. Sivaperumal
Keyword(s):  
Human Serum ◽  
Pesticide Residue ◽  
Pesticide Residues ◽  
Method Development ◽  
Residue Analysis ◽  
Extraction Procedure ◽  
Pesticide Residue Analysis ◽  
Serum Samples ◽  
Human Serum Samples ◽  
Vital Functions

In recent years, exposure to pesticides has gained widespread attention due to their adverse health effects. Long-term exposure to pesticides has shown hazardous effects on vital functions of the human nervous and reproductive systems. Therefore, it is crucial to determine the extent of pesticide exposure in humans. Primarily, it is quite challenging to determine trace levels of pesticide residues in biological matrices. Hence, a quick, multi-residue extraction procedure was experimented for pesticide residue analysis in human serum. Herein, the original QuEChERS extraction method was modified for achieving the best possible recoveries. A total of 15 representative pesticides from each class were selected and fortified into the human serum samples. The extraction was performed by employing acidified solution containing acetonitrile and ethyl acetate followed by vortex and centrifugation. The obtained aqueous layer was collected and vapourised to dryness and d-SPE clean-up was conducted utilising PSA. The extracted sample was injected into the GC-MS/MS system under MRM mode. The method development parameters such as linearity, % RSD, accuracy, LOD, LOQ and % ME were assessed. The results obtained for the serum matrix were found to be within the criteria mentioned in European Union SANTE/12682/2019 guidelines for method validation. The developed solitary method is quick, simple and highly efficient for routine pesticide residue analysis. Hence, a wide spectrum of pesticides can be analysed utilising the proposed method for human serum.

Sign in / Sign up

Export Citation Format

Share Document