Follicle-stimulating hormone increases the expression of tissue inhibitors of metalloproteinases TIMP-1 and TIMP-2 and induces TIMP-1 AP-1 site binding complex(es) in prepubertal rat Sertoli cells.

Endocrinology ◽  
1994 ◽  
Vol 135 (6) ◽  
pp. 2479-2487 ◽  
Author(s):  
S Ulisse ◽  
A R Farina ◽  
D Piersanti ◽  
A Tiberio ◽  
L Cappabianca ◽  
...  
Keyword(s):  
Sertoli Cells ◽  
Follicle Stimulating Hormone ◽  
Tissue Inhibitors Of Metalloproteinases ◽  
Tissue Inhibitors ◽  
Site Binding ◽  
Stimulating Hormone

Follicle-stimulating hormone-dependent estrogen secretion by rat Sertoli cells in vitro: Modulation by calcium

1991 ◽  
Vol 125 (3) ◽  
pp. 280-285 ◽  
Author(s):  
J. Alan Talbot ◽  
Ann Lambert ◽  
Robert Mitchell ◽  
Marek Grabinski ◽  
David C. Anderson ◽  
...  
Keyword(s):  
Sertoli Cells ◽  
Culture Medium ◽  
Follicle Stimulating Hormone ◽  
Dibutyryl Camp ◽  
Immature Rat ◽  
Estradiol Secretion ◽  
Old Rats ◽  
Stimulating Hormone

Abstract We have investigated the role of Ca2+ in the control of FSH-induced estradiol secretion by Sertoli cells isolated from 8-10 days old rats. Exogenous Ca2+ (4-8 mmol/1) inhibited FSH-stimulated E2 secretion such that, with 8 mmol/l Ca2+ and FSH (8 IU/l) E2 secretion decreased from 2091±322 to 1480±84 pmol/l (p<0.002), whilst chelation of Ca2+ in the culture medium with EGTA (3 mmol/l) increased E2 secretion from 360±45 to 1242±133 pmol/l) in the absence of FSH. Further, EGTA (3 mmol/l) markedly potentiated FSH (8 IU/l), forskolin (1 μmol/l) and dibutyryl cAMP (1 mmol/l)-stimulated E2 secretion. Addition of the Ca2+ ionophores, ionomycin (2-5 μmol/l) and A23187 (2 μmol/l), inhibited FSH (8 IU/l)-stimulated E2 secretion by >80%. The effect of ionomycin was totally reversible, whereas that of A23187 was irreversible. Ionomycin (5 μmol/l) had no effect on EGTA-induced E2 secretion in the absence of FSH, but reduced EGTA-provoked E2 secretion by 59% in the presence of FSH (8 IU/l). Similarly, forskolin- and dibutyryl cAMP-provoked E2 production was inhibited 46-50% by ionomycin (5 μmol/l). We conclude that FSH-induced E2 secretion from immature rat Sertoli cells is modulated by intra- and extracellular Ca2+.

Metabolic responses of Sertoli cells in culture to various concentrations of follicle stimulating hormone and cholera toxin

1978 ◽  
Vol 56 (9) ◽  
pp. 875-879 ◽  
Author(s):  
Irving B. Fritz ◽  
Michael D. Griswold ◽  
B. Gregory Louis ◽  
Jennifer H. Dorrington
Keyword(s):  
Cholera Toxin ◽  
Sertoli Cells ◽  
Follicle Stimulating Hormone ◽  
Camp Production ◽  
Apparent Paradox ◽  
Maximal Stimulation ◽  
Enriched Cultures ◽  
Estradiol Synthesis ◽  
Stimulation Of ◽  
Stimulating Hormone

The concentration of cholera toxin required for half-maximal stimulation of cAMP production by Sertoli cell enriched cultures (4.48 × 10−2 μg/ml) is greater than that required for half-maximal stimulation of 17β-estradiol synthesis from testosterone (2.34 × 10−4 μg/ml), [3H]thymidine incorporation into DNA (1.48 × 10−5μg/ml), or androgen binding protein production (2.43 × 10−6 μg/ml). The same relative dose response hierarchy was obtained with respect to stimulation of Sertoli cells with follicle stimulating hormone (FSH) preparations. Again, highest concentrations were required to elicit maximal cAMP production. The data are discussed in relation to an apparent paradox: If cAMP is the mediating 'second messenger' following stimulation by FSH or cholera toxin, why should highest concentrations of these agents be required to elicit 50% of maximal cAMP levels?

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