Localization of messenger ribonucleic acids for insulin-like growth factor I (IGF-I), IGF-II, and the type 1 IGF receptor in the ovine ovary throughout the estrous cycle.

Endocrinology ◽  
1995 ◽  
Vol 136 (12) ◽  
pp. 5266-5273 ◽  
Author(s):  
C M Perks ◽  
P A Denning-Kendall ◽  
R S Gilmour ◽  
D C Wathes
Keyword(s):  
Growth Factor ◽  
Estrous Cycle ◽  
Insulin Like Growth Factor ◽  
Ribonucleic Acids ◽  
Factor I ◽  
Igf I ◽  
Igf Receptor ◽  
Growth Factor I

scholarly journals The Effect of Insulin-Like Growth Factor-I of Pregnant Crossbred Mare Serum and Insulin-Like Growth Factor-I Recombinant Mouse on Estrous Cycles and Litter Sizes of Mice (Mus musculus)

2017 ◽  
Vol 3 (6) ◽  
pp. 175
Author(s):  
Tjuk Imam Restiadi ◽  
Imam Mustofa ◽  
Suzanita Utama ◽  
Sri Mulyati
Keyword(s):  
Growth Factor ◽  
Estrous Cycle ◽  
Litter Size ◽  
Mus Musculus ◽  
Insulin Like Growth Factor ◽  
Estrous Cycles ◽  
Factor I ◽  
Recombinant Mouse ◽  
Igf I ◽  
Growth Factor I

The research was conducted to determine the effect of insulin-like growth factor-I (IGF-I) derived from pregnant crossbred mare serum (PMS) on estrous cycle and litter size in mice (Mus musculus). The research was arranged by Completely Randomized Design (CRD) with seven treatments and five replications. The treatments were control (CO)= physiological NaCl, treatment (T) 1 = 10 ng/ml of IGF-I pregnant mares crossbred serum (PMS), T2 = 20 ng/ml of IGF-I PMS, T3 = 40 ng/ml of IGF-I PMS, T4 = 10 ng/ml of IGF-I recombinant mouse, T5 = 20 ng/ml of IGF-I recombinant mouse, and T6 = 40 ng/ml of IGF-I recombinant mouse. Serie I the subject of this research were 35 female mice. Observed variables included estrous cycle phases that proestrus, estrus, metestrus, and diestrus phase. Serie II for the objects of this research were 35 female mice and 21 male mice in the same treatment on litter size in mice. The data were analyzed by Analysis of Variance (ANOVA), followed by HSD (Honestly Significant Difference) test. The result showed that the addition of IGF-I PMS did not significantly affect (p>0.05) on proestrus, metestrus, and diestrus phase. The addition of IGF-I PMS 40 ng/ml was extended (p<0.05) the estrus phase. It showed that there was not significantly difference (p>0.05) between the effect of IGF-I PMS and IGF-I recombinant mouse with the mice estrous cycles. The result of IGF-I derived from PMS and IGF-I recombinant mouse on litter size in mice did not significantly difference (p>0.05). Test results shows significant differences between control and 40 ng/ml of IGF-I PMS. Keywords: IGF-I, IGF-I pregnant crossbred mare serum, estrous cycle, litter sizes, Mus musculus

Mice carrying null mutations of the genes encoding insulin-like growth factor I (Igf-1) and type 1 IGF receptor (Igf1r)

Cell ◽  
1993 ◽  
Vol 75 (1) ◽  
pp. 59-72 ◽  
Author(s):  
Jeh-Ping Liu ◽  
Julie Baker ◽  
Archlbald S. Perkins ◽  
Elizabeth J. Robertson ◽  
Argiris Efstratiadis
Keyword(s):  
Growth Factor ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Genes Encoding ◽  
Igf Receptor ◽  
Growth Factor I

scholarly journals Osteo-promoting effects of insulin-like growth factor I (IGF-I) in a mouse model of type 1 diabetes

Bone ◽  
2013 ◽  
Vol 57 (1) ◽  
pp. 36-40 ◽  
Author(s):  
John L. Fowlkes ◽  
Jeffry S. Nyman ◽  
R. Clay Bunn ◽  
Chanhee Jo ◽  
Elizabeth C. Wahl ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Growth Factor ◽  
Mouse Model ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Igf I ◽  
Growth Factor I

Receptor binding of insulin-like growth factor-I to mammary microsomes from non-pregnant, pregnant and lactating sheep

1993 ◽  
Vol 136 (2) ◽  
pp. 297-304 ◽  
Author(s):  
S. J. Winder ◽  
S. D. Wheatley ◽  
I. A. Forsyth
Keyword(s):  
Growth Factor ◽  
Membrane Protein ◽  
Binding Affinity ◽  
Binding Sites ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Pregnant Sheep ◽  
Igf I ◽  
Igf Receptor

ABSTRACT Sucrose density centrifugation was used to prepare a partially purified membrane fraction from the mammary glands of non-pregnant, pregnant and lactating sheep. The binding of125 I-labelled insulin-like growth factor-I (IGF-I) was dependent on membrane protein concentration, pH, time and temperature. The binding showed the characteristics of a type-1 IGF receptor, being displaced by IGF-I (median effective dose (ED50) 0·55 nmol/l), less effectively by IGF-II (ED50 8·8 nmol/l) and least effectively by insulin. Glucagon, ovine prolactin and ovine placental lactogen could not displace binding. A molecular weight of 135 000 was determined by affinity cross-linking using disuccinimidyl suberate; this was consistent with the reported size of the type-1 receptor α-subunit. Scatchard analysis was used to determine binding affinity and numbers of IGF-I-binding sites. A single class of high-affinity binding sites was found in all physiological states. In non-pregnant sheep and sheep at days 40, 75 and 110–120 of pregnancy and at term, the binding affinity was similar (apparent dissociation constant (Kd) 2·73 ±0·31 nmol/l, n = 22). In lactating sheep (weeks 1, 4 and 10), the binding affinity was significantly (P = 0·02) higher (Kd 0·77± 0·06 nmol/l n = 9). Binding capacity was similar in non-pregnant and pregnant sheep (1005 ± 113 fmol/mg, n = 19), but fell by parturition and remained low in lactation (570±52 fmol/mg membrane protein, n = 12). The results suggest that the mammary growth of pregnancy is not regulated at the level of the type-1 IGF receptor. Journal of Endocrinology (1993) 136, 297–304

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