Cytokine modulation of progesterone and estradiol secretion in cultures of luteinized human granulosa cells.

1992 ◽  
Vol 75 (1) ◽  
pp. 254-258
Author(s):  
M Fukuoka ◽  
K Yasuda ◽  
N Emi ◽  
H Fujiwara ◽  
M Iwai ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Cytokine Modulation ◽  
Human Granulosa Cells ◽  
Estradiol Secretion

The effect of histamine on progesterone and estradiol secretion of human granulosa cells in serum-free culture

1993 ◽  
Vol 7 (4) ◽  
pp. 235-239 ◽  
Author(s):  
J. Bódis ◽  
H.-R. Tinneberg ◽  
H. Schwarz ◽  
F. Papenfuß ◽  
A. Török ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Serum Free ◽  
Human Granulosa Cells ◽  
Estradiol Secretion

Effect of noradrenaline and dopamine on progesterone and estradiol secretion of human granulosa cells

1993 ◽  
Vol 129 (2) ◽  
pp. 165-168 ◽  
Author(s):  
József Bódis ◽  
Hans R Tinneberg ◽  
Attila Török ◽  
Philippe Cledon ◽  
Volker Hanf ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Direct Action ◽  
Serum Free Medium ◽  
Free Medium ◽  
Vitro Fertilization ◽  
Serum Free ◽  
Human Granulosa Cells ◽  
Estradiol Secretion ◽  
Cells Cultured

The aim of this study was to explore the direct action of noradrenaline and dopamine on progesterone and estradiol secretion of human granulosa cells cultured in serum-free medium. Progesterone and estradiol production was measured in the presence and absence of noradrenaline, dopamine or propranolol using radioimmunoassays; statistical analysis was performed by analysis of variance and Newman-Keul's multiple range test. Twenty-six women aged 31±3 years undergoing in vitro fertilization and embryo transfer for infertility treatment at University Women's Hospital, University of Tübingen, Germany, took part in this study. Noradrenaline significantly inhibited progesterone production by human granulosa cells in a dose-related manner at a concentration of 10−4–10−6 mol/l. Dopamine significantly stimulated estradiol secretion by granulosa cells in an inverse dose-related manner. Both effects were blocked by propranolol. The results suggest that catecholaminergic actions switch over the steroid production of human granulosa cells cultured in serum-free medium from progesterone to estradiol.

In vivo and in vitro effect of growth hormone on estradiol secretion by human granulosa cells.

1993 ◽  
Vol 77 (1) ◽  
pp. 61-67 ◽  
Author(s):  
A Barreca ◽  
P G Artini ◽  
P Del Monte ◽  
P Ponzani ◽  
P Pasquini ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Granulosa Cells ◽  
Human Granulosa Cells ◽  
Estradiol Secretion ◽  
Vitro Effect

scholarly journals Chemerin inhibits IGF-1-induced progesterone and estradiol secretion in human granulosa cells

2012 ◽  
Vol 27 (6) ◽  
pp. 1790-1800 ◽  
Author(s):  
M. Reverchon ◽  
M. Cornuau ◽  
C. Rame ◽  
F. Guerif ◽  
D. Royere ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Human Granulosa Cells ◽  
Estradiol Secretion

Herbimycin, a Tyrosine Kinase Inhibitor with Src Selectivity, Reduces Progesterone and Estradiol Secretion by Human Granulosa Cells

Endocrine ◽  
2001 ◽  
Vol 15 (3) ◽  
pp. 271-276 ◽  
Author(s):  
Valerie Montgomery Rice ◽  
Ahsan R Chaudhery ◽  
Okunola Oluola ◽  
Sharon D Limback ◽  
Katherine F Roby ◽  
...  
Keyword(s):  
Tyrosine Kinase ◽  
Tyrosine Kinase Inhibitor ◽  
Granulosa Cells ◽  
Kinase Inhibitor ◽  
Human Granulosa Cells ◽  
Herbimycin A ◽  
Estradiol Secretion

Influence of serotonin on progesterone and estradiol secretion of cultured human granulosa cells**Supported by the Alexander von Humboldt Foundation (AvH), Bonn, Germany.

1992 ◽  
Vol 57 (5) ◽  
pp. 1008-1011 ◽  
Author(s):  
József Bódis ◽  
Attila Török ◽  
Hans-Rudolf Tinneberg ◽  
Volker Hanf ◽  
Miklos Hamori ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Human Granulosa Cells ◽  
Alexander Von Humboldt ◽  
Estradiol Secretion

In vitro Stimulation of Granulosa Cells by a Combination of Different Active Ingredients of Unkei-to

2004 ◽  
Vol 32 (04) ◽  
pp. 569-578 ◽  
Author(s):  
Wen-Shu Sun ◽  
Atsushi Imai ◽  
Keiko Tagami ◽  
Michiyo Sugiyama ◽  
Tatsuro Furui ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Response Curves ◽  
Vitro Fertilization ◽  
Human Granulosa Cells ◽  
Estradiol Secretion ◽  
Paeoniae Radix ◽  
In Vivo Effects ◽  
Stimulation Of

Unkei-to is widely used in traditional Japanese herbal medicine for its ovulation-inducing effect. In the present study, we investigated the in vivo effects of Unkei-to and its compounds on the steroidogenesis and cytokine secretion in human granulosa cells. Unkei-to stimulated the secretions of 17β-estradiol and progesterone from highly luteinized granulosa cells obtained from in vitro fertilization patients; the stimulated effect on estradiol secretion occurred with 0.3 μg/ml, while a significant effect on progesterone secretion was obtained at 10 μg/ml. The Unkei-to stimulation of estradiol secretion could be accounted for by the effects of its ingredients, Shakuyaku (paeoniae radix, Paeonia lactiflora Pallas) and Keihi (cinnamomi cortex, Cinnamomum cassia Blume); while dose response curves for Unkei-to and Keihi to induce progesterone production were superimposable. Exposure of the cells to Unkei-to caused dose-dependent increases in the concentrations of interleukin (IL)-1β, IL-6 and IL-8 in the culture medium. Similar results were obtained when cells were incubated with the ingredient Ninjin (ginseng radix, Panax ginseng C.A. Meyer), but not Shakuyaku and Keihi. These results indicate that Unkei-to has direct stimulatory effects on human granulosa cells to stimulate the steroidogenesis and secretion of cytokines (IL-1β, IL-6 and IL-8). The various beneficial actions of Unkei-to on the ovary may result from a combination of different ingredient herbs with different stimulatory effects on both steroidogenesis and the ovulatory process within the ovary, as well as stimulatory effect on the hypothalamus-pituitary axis.

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