In vivo and in vitro effect of growth hormone on estradiol secretion by human granulosa cells.

Unkei-to is widely used in traditional Japanese herbal medicine for its ovulation-inducing effect. In the present study, we investigated the in vivo effects of Unkei-to and its compounds on the steroidogenesis and cytokine secretion in human granulosa cells. Unkei-to stimulated the secretions of 17β-estradiol and progesterone from highly luteinized granulosa cells obtained from in vitro fertilization patients; the stimulated effect on estradiol secretion occurred with 0.3 μg/ml, while a significant effect on progesterone secretion was obtained at 10 μg/ml. The Unkei-to stimulation of estradiol secretion could be accounted for by the effects of its ingredients, Shakuyaku (paeoniae radix, Paeonia lactiflora Pallas) and Keihi (cinnamomi cortex, Cinnamomum cassia Blume); while dose response curves for Unkei-to and Keihi to induce progesterone production were superimposable. Exposure of the cells to Unkei-to caused dose-dependent increases in the concentrations of interleukin (IL)-1β, IL-6 and IL-8 in the culture medium. Similar results were obtained when cells were incubated with the ingredient Ninjin (ginseng radix, Panax ginseng C.A. Meyer), but not Shakuyaku and Keihi. These results indicate that Unkei-to has direct stimulatory effects on human granulosa cells to stimulate the steroidogenesis and secretion of cytokines (IL-1β, IL-6 and IL-8). The various beneficial actions of Unkei-to on the ovary may result from a combination of different ingredient herbs with different stimulatory effects on both steroidogenesis and the ovulatory process within the ovary, as well as stimulatory effect on the hypothalamus-pituitary axis.

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In vitro effect of dehydroepiandrosterone sulfate on steroid receptors, aromatase, cyclooxygenase-2 expression, and steroid hormone production in preovulatory human granulosa cells

Fertility and Sterility ◽

10.1016/j.fertnstert.2007.01.008 ◽

2007 ◽

Vol 88 (4) ◽

pp. 1135-1142 ◽

Cited By ~ 5

Author(s):

Khalid ELBeltagy ◽

Ken-ichi Honda ◽

Koji Ozaki ◽

Takuya Misugi ◽

Osamu Tokuyama ◽

...

Keyword(s):

Granulosa Cells ◽

Steroid Receptors ◽

Steroid Hormone ◽

Dehydroepiandrosterone Sulfate ◽

Cyclooxygenase 2 ◽

Hormone Production ◽

Human Granulosa Cells ◽

Vitro Effect

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Effect of noradrenaline and dopamine on progesterone and estradiol secretion of human granulosa cells

Acta Endocrinologica ◽

10.1530/acta.0.1290165 ◽

1993 ◽

Vol 129 (2) ◽

pp. 165-168 ◽

Cited By ~ 15

Author(s):

József Bódis ◽

Hans R Tinneberg ◽

Attila Török ◽

Philippe Cledon ◽

Volker Hanf ◽

...

Keyword(s):

Granulosa Cells ◽

Direct Action ◽

Serum Free Medium ◽

Free Medium ◽

Vitro Fertilization ◽

Serum Free ◽

Human Granulosa Cells ◽

Estradiol Secretion ◽

Cells Cultured

The aim of this study was to explore the direct action of noradrenaline and dopamine on progesterone and estradiol secretion of human granulosa cells cultured in serum-free medium. Progesterone and estradiol production was measured in the presence and absence of noradrenaline, dopamine or propranolol using radioimmunoassays; statistical analysis was performed by analysis of variance and Newman-Keul's multiple range test. Twenty-six women aged 31±3 years undergoing in vitro fertilization and embryo transfer for infertility treatment at University Women's Hospital, University of Tübingen, Germany, took part in this study. Noradrenaline significantly inhibited progesterone production by human granulosa cells in a dose-related manner at a concentration of 10−4–10−6 mol/l. Dopamine significantly stimulated estradiol secretion by granulosa cells in an inverse dose-related manner. Both effects were blocked by propranolol. The results suggest that catecholaminergic actions switch over the steroid production of human granulosa cells cultured in serum-free medium from progesterone to estradiol.

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THE PRODUCTION OF PROGESTERONE, ANDROGENS AND OESTROGENS BY HUMAN GRANULOSA CELLS IN VITRO AND IN VIVO

Hormonal Steroids ◽

10.1016/b978-0-08-023796-1.50111-0 ◽

1979 ◽

pp. 775-779

Author(s):

K.P. MCNATTY ◽

A. MAKRIS ◽

C. DE GRAZIA ◽

R. OSATHANONDH ◽

K.J. RYAN

Keyword(s):

Granulosa Cells ◽

Human Granulosa Cells

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INFLUENCE OF 16-ARYLOXYPROSTAGLANDINS ON THE PRODUCTION OF PROGESTERONE BY HUMAN GRANULOSA CELLS IN VITRO

Journal of Endocrinology ◽

10.1677/joe.0.0710259 ◽

1976 ◽

Vol 71 (2) ◽

pp. 259-263 ◽

Cited By ~ 4

Author(s):

K. M. HENDERSON

Keyword(s):

Tissue Culture ◽

Adenylate Cyclase ◽

Granulosa Cells ◽

Farm Animals ◽

Human Granulosa Cells ◽

Progesterone Production ◽

Porcine Granulosa Cells ◽

Prostaglandin F

SUMMARY 16-Aryloxy analogues of prostaglandin F2α(PGF2α) are potent luteolysins in laboratory and farm animals. When their effect on progesterone production by luteinized human granulosa cells in tissue culture was investigated inhibition of both basal and gonadotrophin-stimulated progesterone production was observed, so revealing characteristics expected of potential human luteolysins. The analogues were, however, unable to inhibit progesterone production stimulated by PGE2, suggesting that like PGF2α these compounds may act by specifically blocking LH-activated adenylate cyclase. The 16-aryloxyprostaglandins similarly inhibited progesterone production by porcine granulosa cells, so that the effects observed with the 16-aryloxyprostaglandins in vitro may be indicative of their potential in vivo.

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Prior exposure to gonadotrophins prevents the subsequent antigonadotrophic actions of cloprostenol by a cyclic AMP-dependent mechanism in cultured human granulosa cells

Journal of Endocrinology ◽

10.1677/joe.0.1310319 ◽

1991 ◽

Vol 131 (2) ◽

pp. 319-325 ◽

Cited By ~ 3

Author(s):

A. E. Michael ◽

G. E. Webley

Keyword(s):

Cyclic Amp ◽

Corpus Luteum ◽

Granulosa Cells ◽

Early Pregnancy ◽

Prior Exposure ◽

Vitro Fertilization ◽

Human Granulosa Cells ◽

Dependent Mechanism

ABSTRACT The antigonadotrophic action of a prostaglandin F2α analogue, cloprostenol, has been investigated in human granulosa cells obtained from cycles stimulated for in-vitro fertilization and induced to secrete luteal quantities of progesterone by culture in serum-supplemented medium. Cells were exposed to conditions which may mimic those occurring in early pregnancy to establish the roles of human chorionic gonadotrophin (hCG) versus LH and that of cyclic AMP (cAMP) in the anti-gonadotrophic action of cloprostenol. When human granulosa cells were cultured in the absence of treatment for 3 days, exposure to cloprostenol had no effect on basal progesterone production but inhibited hCG-stimulated progesterone (60% decrease; P<0·01), hCG-stimulated cAMP (40% decrease; P < 0·05) and the progesterone response to dibutyryl cAMP (dbcAMP; 70% decrease; P < 0·01), suggesting pre- and post-cAMP sites of cloprostenol action. The inhibitory actions of cloprostenol were prevented when the granulosa cells were either continuously exposed to treatment from the start of culture or pre-exposed for 3 days to maximum concentrations of LH, hCG, dbcAMP or 8-bromo-cAMP. We conclude that prior exposure either in vivo or in vitro to LH or hCG prevents the subsequent antigonadotrophic action of cloprostenol via a cAMP-dependent mechanism. Prevention of the antigonadotrophic action of cloprostenol after exposure to hCG may be a mechanism through which CG prevents regression of the corpus luteum in early pregnancy, while the suppressive effect of LH pretreatment may account for the refractory response of the early corpus luteum to cloprostenol following the midcycle LH surge. Journal of Endocrinology (1991) 131, 319–325

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