Localization of cytochrome P450 cholesterol side-chain cleavage, cytochrome P450 17 alpha-hydroxylase/17, 20-lyase, and 3 beta-hydroxysteroid dehydrogenase isomerase steroidogenic enzymes in human and rhesus monkey fetal adrenal glands: reappraisal of functional zonation.

1993 ◽  
Vol 77 (5) ◽  
pp. 1184-1189
Author(s):  
S Mesiano ◽  
C L Coulter ◽  
R B Jaffe
Keyword(s):  
Cytochrome P450 ◽  
Rhesus Monkey ◽  
Adrenal Glands ◽  
Hydroxysteroid Dehydrogenase ◽  
Side Chain ◽  
Steroidogenic Enzymes ◽  
Side Chain Cleavage ◽  
Chain Cleavage

Concentrations of cytochrome P450 cholesterol side-chain cleavage enzyme and 3 beta-hydroxysteroid dehydrogenase during prostaglandin F2 alpha-induced luteal regression in cattle

1995 ◽  
Vol 7 (5) ◽  
pp. 1213 ◽  
Author(s):  
RJ Rodgers ◽  
CA Vella ◽  
FM Young ◽  
XC Tian ◽  
JE Fortune
Keyword(s):  
Cytochrome P450 ◽  
Hydroxysteroid Dehydrogenase ◽  
Side Chain ◽  
Corpora Lutea ◽  
Steroidogenic Enzymes ◽  
Rapid Reduction ◽  
Plasma Progesterone ◽  
Side Chain Cleavage ◽  
Prostaglandin F2 Alpha ◽  
Chain Cleavage

Prostaglandin F2 alpha (PGF2 alpha)-induced regression of the corpus luteum causes both plasma progesterone concentrations and luteal concentrations of mRNA encoding the steroidogenic enzyme 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) to fall in parallel. To investigate the hypothesis that a decline in the concentrations of mRNA encoding steroidogenic enzymes causes plasma progesterone to fall, the luteal concentrations of the enzymes 3 beta-HSD and cytochrome P450 cholesterol side-chain cleavage were measured during induced luteolysis. Holstein heifers were treated with PGF2 alpha (25 mg Lutalyse) on Day 6 or Day 7 of the oestrous cycle and corpora lutea were collected 0 h, 2 h, 12 h, and 24 h later (n = 6, 4, 4, and 4 respectively). Analyses of the steroidogenic enzymes were carried out by Western immunoblotting. The luteal concentrations of both steroidogenic enzymes did not decrease over the 24-h period. It is concluded that, although the concentrations of mRNA encoding steroidogenic enzymes may decline in response to PGF2 alpha, this does not lead to a sufficiently rapid reduction in the concentrations of the enzymes to precede, and thus cause, the decline in plasma progesterone concentrations. Thus, the mechanism for the initial decline in plasma progesterone concentrations during luteolysis is still not known.

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