Binding of a member of the NF1 family of transcription factors to two distinct cis-acting elements in the promoter and 5'-flanking region of the human cellular retinol binding protein 1 gene

1994 ◽  
Vol 8 (6) ◽  
pp. 732-745 ◽  
Author(s):  
W. Eskild
Keyword(s):  
Transcription Factors ◽  
Binding Protein ◽  
Retinol Binding Protein ◽  
Cis Acting ◽  
Flanking Region ◽  
Cellular Retinol Binding Protein

Analysis of human cellular retinol-binding protein II promoter during enterocyte differentiation

2002 ◽  
Vol 282 (6) ◽  
pp. G1079-G1087 ◽  
Author(s):  
Liang Zhang ◽  
Xueping E ◽  
Kathryn E. Luker ◽  
Jian-Su Shao ◽  
Marc S. Levin ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Binding Protein ◽  
Retinol Binding Protein ◽  
Direct Sequence Analysis ◽  
Selective Agonists ◽  
Flanking Region ◽  
Small Intestinal ◽  
Small Intestinal Villus ◽  
Enterocyte Differentiation ◽  
Cellular Retinol Binding Protein

Cellular retinol binding protein II (CRBP II) is a vitamin A-binding protein that is expressed specifically in small intestinal villus absorptive cells. Previous studies have shown that retinoic acid upregulates endogenous human CRBP II gene expression in differentiated Caco-2 cells. To better characterize the regulation of human CRBP II expression, we analyzed the ability of receptor-selective agonists to enhance transcription from the 5′-upstream flanking region of the human CRBP II gene. Stable transfection experiments showed that the proximal 2.8-kb region of the human CRBP II gene is sufficient for retinoic acid inducibility in differentiated Caco-2 cells. However, direct sequence analysis and transient transfection experiments indicate that, unlike the rat CRBP II promoter, the human CRBP II promoter is not a direct retinoid X receptor target. The results indicate that the retinoic acid responsiveness of the human CRBP II promoter is mediated by an indirect mechanism and that this mechanism is associated with enterocyte differentiation.

scholarly journals Cellular Retinol-Binding Protein Type I (CRBP-I) Regulates Adipogenesis

2010 ◽  
Vol 30 (14) ◽  
pp. 3412-3420 ◽  
Author(s):  
C. F. Zizola ◽  
S. K. Frey ◽  
S. Jitngarmkusol ◽  
B. Kadereit ◽  
N. Yan ◽  
...  
Keyword(s):  
Transcription Factors ◽  
High Fat Diet ◽  
Adipocyte Differentiation ◽  
Binding Protein ◽  
Retinol Binding Protein ◽  
Type I ◽  
High Fat ◽  
Embryonic Fibroblasts ◽  
Glucose Tolerant ◽  
Cellular Retinol Binding Protein

ABSTRACT Adipogenesis is governed by a well-documented cascade of transcription factors. However, less is known about non-transcription factors that govern early stages of adipogenesis. Here we show that cellular retinol-binding protein type I (CRBP-I), a small cytosolic binding protein for retinol and retinaldehyde, is specifically restricted to preadipocytes in white adipose tissue. The absence of CRBP-I in mice (CRBP-I-KO mice) leads to increased adiposity. Despite increased adiposity, CRBP-I-KO mice remain more glucose tolerant and insulin sensitive during high-fat-diet feeding. 3T3-L1 cells deficient in CRBP-I or mouse embryonic fibroblasts derived from CRBP-I-KO mice had increased adipocyte differentiation and triglyceride (TG) accumulation. This was due to increased expression and activity of PPARγ, while other transcription factor pathways in early and late differentiation remained unchanged. Conversely, the overexpression of CRBP-I in 3T3-L1 cells results in decreased TG accumulation. In conclusion, CRBP-I is a cytosolic protein specifically expressed in preadipocytes that regulates adipocyte differentiation in part by affecting PPARγ activity.

scholarly journals Molecular basis for the interaction of cellular retinol binding protein 2 (CRBP2) with nonretinoid ligands

2021 ◽  
Vol 62 ◽  
pp. 100054
Author(s):  
Josie A. Silvaroli ◽  
Jacqueline Plau ◽  
Charlie H. Adams ◽  
Surajit Banerjee ◽  
Made Airanthi K. Widjaja-Adhi ◽  
...  
Keyword(s):  
Molecular Basis ◽  
Binding Protein ◽  
Retinol Binding Protein ◽  
Cellular Retinol Binding Protein

Fluorescence studies of rat cellular retinol binding protein II produced in Escherichia coli: an analysis of four tryptophan substitution mutants

Biochemistry ◽  
1992 ◽  
Vol 31 (8) ◽  
pp. 2376-2383 ◽  
Author(s):  
Bruce C. Locke ◽  
Jean M. MacInnis ◽  
Shi Jun Qian ◽  
Jeffrey I. Gordon ◽  
Ellen Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Binding Protein ◽  
Retinol Binding Protein ◽  
Fluorescence Studies ◽  
Cellular Retinol Binding Protein

scholarly journals Teq I RFLP in the human cellular retinol-binding protein (CRBP) gene

1988 ◽  
Vol 16 (15) ◽  
pp. 7758-7758
Author(s):  
A. Pellegrino ◽  
S. Garofalo ◽  
S. Cocozza ◽  
A. Monticelli ◽  
R. Faraonio ◽  
...  
Keyword(s):  
Binding Protein ◽  
Retinol Binding Protein ◽  
Cellular Retinol Binding Protein
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