scholarly journals Inhibitory Effect of Estrogens on GCDFP-15 mRNA Levels and Secretion in ZR-75-1 Human Breast Cancer Cells

1989 ◽  
Vol 3 (4) ◽  
pp. 694-702 ◽  
Author(s):  
Jacques Simard ◽  
Anne Catherine Hatton ◽  
Claude Labrief ◽  
Sophie Dauvois ◽  
Hui Fen Zhao ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Inhibitory Effect ◽  
Mrna Levels ◽  
Human Breast Cancer Cells ◽  
Human Breast

Estrogen Activities and the Cellular Effects of Natural Progesterone from Wild Yam Extract in MCF-7 Human Breast Cancer Cells

2009 ◽  
Vol 37 (01) ◽  
pp. 159-167 ◽  
Author(s):  
Mi-Kyung Park ◽  
Hyeok-Yi Kwon ◽  
Woong-Shick Ahn ◽  
Sumi Bae ◽  
Mee-Ra Rhyu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Estrogenic Activity ◽  
Mrna Levels ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Wild Yam ◽  
Mcf 7

We studied the estrogenic activity and cellular effect of wild yam extract in MCF-7 human breast cancer cells. The extract increased the activity of the progesterone receptor and pS2 genes at the mRNA levels in human breast cancer MCF-7 cells, although the effects were not as prominent as those of 17β-estradiol (E2). Western blot analysis showed that the level of estrogen receptor α protein was down-regulated after treatment with E2 or wild yam extract. Wild yam extract also inhibited proliferation of MCF-7 cells. These data indicate that wild yam extract acts as a weak phytoestrogen and protects against proliferation in human breast carcinoma MCF-7 cells.

Low-level lasers on microRNA and uncoupling protein 2 mRNA levels in human breast cancer cells

Laser Physics ◽  
2017 ◽  
Vol 27 (6) ◽  
pp. 065601 ◽  
Author(s):  
K S Canuto ◽  
A F Teixeira ◽  
J A Rodrigues ◽  
F Paoli ◽  
E M Nogueira ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Uncoupling Protein ◽  
Uncoupling Protein 2 ◽  
Mrna Levels ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Low Level

scholarly journals Studies on Multifunctional Effect of All-Trans Retinoic Acid (ATRA) on Matrix Metalloproteinase-2 (MMP-2) and Its Regulatory Molecules in Human Breast Cancer Cells (MCF-7)

2009 ◽  
Vol 2009 ◽  
pp. 1-13 ◽  
Author(s):  
Anindita Dutta ◽  
Triparna Sen ◽  
Aniruddha Banerji ◽  
Shamik Das ◽  
Amitava Chatterjee
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Gelatin Zymography ◽  
Inhibitory Effect ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Atra Treatment ◽  
Mcf 7

Background. Vitamin A derivative all-trans retinoic acid (ATRA) is considered as a potent chemotherapeutic drug for its capability of regulating cell growth and differentiation. We studied the effect of ATRA on MMP-2 in MCF-7, human breast cancer cells, and the probable signaling pathways which are affected by ATRA on regulating pro-MMP-2 activity and expression.Methods. Gelatin zymography, RT-PCR, ELISA, Western blot, Immunoprecipitation, and Cell adhesion assay are used.Results. Gelatin zymography showed that ATRA caused a dose-dependent inhibition of pro-MMP-2 activity. ATRA treatment downregulates the expression of MT1-MMP, EMMPRIN, FAK, NF-kB, and p-ERK. However, expression of E-cadherin, RAR, and CRABP increased upon ATRA treatment. Binding of cells to extra cellular matrix (ECM) protein fibronectin reduced significantly after ATRA treatment.Conclusions. The experimental findings clearly showed the inhibition of MMP-2 activity upon ATRA treatment. This inhibitory effect of ATRA on MMP-2 activity in human breast cancer cells (MCF-7) may result due to its inhibitory effect on MT1-MMP, EMMPRIN, and upregulation of TIMP-2. This study is focused on the effect of ATRA on MMP, MMP-integrin-E-cadherin interrelationship, and also the effect of the drug on different signaling molecules which may involve in the progression of malignant tumor development.

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